Integrated Proteomics and Metabolomics Suggests Symbiotic Metabolism and Multimodal Regulation in a Fungal-Endobacterial System: Symbiotic Metabolism and Multimodal Regulation

Li, Zhou; Yao, Qiuming; Dearth, Stephen P.; Entler, Matthew R.; Castro Gonzalez, Hector F.; Uehling, Jessie K.; Vilgalys, Rytas J.; Hurst, Gregory B.; Campagna, Shawn R.; Labbé, Jessy L.; Pan, Chongle

doi:10.1111/1462-2920.13605

Integrated Proteomics and Metabolomics Suggests Symbiotic Metabolism and Multimodal Regulation in a Fungal-Endobacterial System: Symbiotic Metabolism and Multimodal Regulation

Journal Article · Sun Nov 20 23:00:00 EST 2016 · Environmental Microbiology

DOI:https://doi.org/10.1111/1462-2920.13605· OSTI ID:1339384

Li, Zhou ^[1]; Yao, Qiuming ^[1]; Dearth, Stephen P. ^[2]; Entler, Matthew R. ^[3]; Castro Gonzalez, Hector F. ^[2]; Uehling, Jessie K. ^[4]; Vilgalys, Rytas J. ^[4]; Hurst, Gregory B. ^[1]; Campagna, Shawn R. ^[2]; Labbé, Jessy L. ^[1]; Pan, Chongle ^[1]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Univ. of Tennessee, Knoxville, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Duke Univ., Durham, NC (United States)

Many plant-associated fungi host endosymbiotic endobacteria with reduced genomes. While endobacteria play important roles in these tri-partite plant-fungal-endobacterial systems, the active physiology of fungal endobacteria has not been characterized extensively by systems biology approaches. Here in this paper, we use integrated proteomics and metabolomics to characterize the relationship between the endobacterium Mycoavidus sp. and the root-associated fungus Mortierella elongata. In nitrogen-poor media, M. elongata had decreased growth but hosted a large and growing endobacterial population. The active endobacterium likely extracted malate from the fungal host as the primary carbon substrate for energy production and biosynthesis of phospho-sugars, nucleobases, peptidoglycan, and some amino acids. The endobacterium obtained nitrogen by importing a variety of nitrogen-containing compounds. Further, nitrogen limitation significantly perturbed the carbon and nitrogen flows in the fungal metabolic network. M. elongata regulated many pathways by concordant changes on enzyme abundances, post-translational modifications, reactant concentrations, and allosteric effectors. Lastly, such multimodal regulations may be a general mechanism for metabolic modulation.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Oak Ridge Leadership Computing Facility (OLCF); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1339384

Alternate ID(s):: OSTI ID: 1399551

Journal Information:: Environmental Microbiology, Journal Name: Environmental Microbiology Journal Issue: 1 Vol. 12; ISSN 1462-2912

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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