Effects of Low Concentrations of Docosahexaenoic Acid on the Structure and Phase Behavior of Model Lipid Membranes

Lor, Chai; Hirst, Linda

doi:10.3390/membranes5040857

Effects of Low Concentrations of Docosahexaenoic Acid on the Structure and Phase Behavior of Model Lipid Membranes

Journal Article · Fri Dec 04 00:00:00 EST 2015 · Membranes

DOI:https://doi.org/10.3390/membranes5040857· OSTI ID:1630035

Lor, Chai ^[1]; Hirst, Linda ^[2]

Univ. of California, Merced, CA (United States); DOE/OSTI
Univ. of California, Merced, CA (United States)

In this paper we report an X-ray diffraction study on the phase behavior of binary lipid mixtures of 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (DHA-PE) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) at low concentrations below 5.0 mol% DHA-PE. Our results show that DHA-PE induces phase separation into a DHA rich liquid crystalline (L_α) phase and a DHA poor gel (L_β') phase at overall DHA-PE concentrations as low as 0.1 mol%. In addition, we find that the structure of the L_β' phase, from which the DHA-PE molecules are largely excluded, is modified in the phase separated state at low DHA-PE concentrations, with a decrease in bilayer thickness of 1.34 nm for 0.1 mol% at room temperature, compared to pure DPPC bilayers. This result is contrary to that seen in similar studies on mono-unsaturated lipids where an increase in bilayer thickness is observed. The surprising effect of such low DHA-PE concentrations on membrane structure may be important in understanding the role of highly polyunsaturated lipids in biological membrane-based structures and similar artificial surfactant systems.

View Accepted Manuscript (DOE)

Research Organization:: SLAC National Accelerator Laboratory, Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)

Sponsoring Organization:: National Science Foundation (NSF); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1630035

Journal Information:: Membranes, Journal Name: Membranes Journal Issue: 4 Vol. 5; ISSN 2077-0375; ISSN MBSEB6

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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X-ray diffraction
gel phase
lipid bilayer

Effects of Low Concentrations of Docosahexaenoic Acid on the Structure and Phase Behavior of Model Lipid Membranes

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