The effects of vinblastine on on isolated Xenopus oocytes
Journal Article
·
· Journal of Cell Biology
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biology Division; DOE/OSTI
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biology Division
A recent survey of the effects of various physical and chemical parameters on yolk protein incorporation by isolated Xenopus oocytes indicates that the Vinca alkaloid vinblastine sulfate (VLB) is among the more effective inhibitors of this process. The Vinca alkaloids have received considerable attention because of their ability to disrupt the integrity of microtubules; therefore, they appear to be useful tools for examining the functions of microtubules in a variety of cells. Furthermore, they facilitate the isolation and purification of microtubular proteins for biochemical studies. These properties of VLB, combined with its marked depressant effect on protein uptake by isolated oocytes, led us to examine VLB-treated oocytes for altered cytological characteristics. This communication describes alterations induced by VLB and discusses their possible significance in terms of the relationship of microtubules to the micropinocytotic uptake of protein.
- Research Organization:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
- Grant/Contract Number:
- AC05-00OR22725
- OSTI ID:
- 1629490
- Journal Information:
- Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 2 Vol. 53; ISSN 0021-9525
- Publisher:
- Rockefeller University PressCopyright Statement
- Country of Publication:
- United States
- Language:
- English
Protein incorporation by isolated amphibian oocytes III. Optimum incubation conditions
|
journal | June 1973 |
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