Functional expression of murine multidrug resistance in Xenopus laevis oocytes

Journal Article · Fri Jun 01 04:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.12.4737· OSTI ID:6151671

Castillo, G; Vera, J C; Rosen, O M ^[1]; Yang, Chiaping Huang; Horwitz, S B ^[2]

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1. Memorial Sloan-Kettering Cancer Research Center, New York, NY (USA)
2. Albert Einstein College of Medicine, Bronx, NY (USA)

The development of multidrug resistance (MDR) is associated with the overproduction of a plasma membrane glycoprotein, P glycoprotein. Here the authors report the functional expression of a member of the murine MDR family of proteins and show that Xenopus oocytes injected with RNA encoding the mouse mdr1b P glycoprotein develop a MDR-like phenotype. Immunological analysis indicated that oocytes injected with the mdr1b RNA synthesized a protein with the size and immunological characteristics of the mouse mdr1b P glycoprotein. These oocytes exhibited a decreased accumulation of ({sup 3}H)vinblastine and showed an increased capacity to extrude the drug compared to control oocytes not expressing the P glycoprotein. In addition, competition experiments indicated that verapamil, vincristine, daunomycin, and quinidine, but not colchicine, can overcome the rapid drug efflux conferred by the expression of the mouse P glycoprotein.

• OSTI ID: 6151671
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:12; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALOIDS
AMPHIBIANS
ANIMALS
ANTIMITOTIC DRUGS
ANTIPYRETICS
AQUATIC ORGANISMS
AROMATICS
AZAARENES
AZOLES
CENTRAL NERVOUS SYSTEM DEPRESSANTS
COLCHICINE
DRUGS
GENE REGULATION
GENES
GERM CELLS
GLYCOPROTEINS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
IMMUNOLOGY
INDOLES
MEMBRANE PROTEINS
MESSENGER-RNA
NUCLEIC ACIDS
OOCYTES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRROLES
RNA
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES
VINBLASTINE