Performance Evaluation of Fast Microfluidic Thermal Lysis of Bacteria for Diagnostic Sample Preparation

Packard, Michelle; Wheeler, Elizabeth; Alocilja, Evangelyn; Shusteff, Maxim

doi:10.3390/diagnostics3010105

Performance Evaluation of Fast Microfluidic Thermal Lysis of Bacteria for Diagnostic Sample Preparation

Journal Article · Wed Jan 16 23:00:00 EST 2013 · Diagnostics

DOI:https://doi.org/10.3390/diagnostics3010105· OSTI ID:1629119

Packard, Michelle ^[1]; Wheeler, Elizabeth ^[2]; Alocilja, Evangelyn ^[3]; Shusteff, Maxim ^[2]

Michigan State Univ., East Lansing, MI (United States). Nano-Biosensors Lab.; Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); DOE/OSTI
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Michigan State Univ., East Lansing, MI (United States). Nano-Biosensors Lab.

Development of new diagnostic platforms that incorporate lab-on-a-chip technologies for portable assays is driving the need for rapid, simple, low cost methods to prepare samples for downstream processing or detection. An important component of the sample preparation process is cell lysis. In this work, a simple microfluidic thermal lysis device is used to quickly release intracellular nucleic acids and proteins without the need for additional reagents or beads used in traditional chemical or mechanical methods (e.g., chaotropic salts or bead beating). On-chip lysis is demonstrated in a multi-turn serpentine microchannel with external temperature control via an attached resistive heater. Lysis was confirmed for Escherichia coli by fluorescent viability assay, release of ATP measured with bioluminescent assay, release of DNA measured by fluorometry and qPCR, as well as bacterial culture. Results comparable to standard lysis techniques were achievable at temperatures greater than 65 °C and heating durations between 1 and 60 s.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division; Science, Mathematics and Research Transformation (SMART)

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 1629119

Journal Information:: Diagnostics, Journal Name: Diagnostics Journal Issue: 1 Vol. 3; ISSN 2075-4418

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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