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Title: Microfluidic device for acoustic cell lysis

Abstract

A microfluidic acoustic-based cell lysing device that can be integrated with on-chip nucleic acid extraction. Using a bulk acoustic wave (BAW) transducer array, acoustic waves can be coupled into microfluidic cartridges resulting in the lysis of cells contained therein by localized acoustic pressure. Cellular materials can then be extracted from the lysed cells. For example, nucleic acids can be extracted from the lysate using silica-based sol-gel filled microchannels, nucleic acid binding magnetic beads, or Nafion-coated electrodes. Integration of cell lysis and nucleic acid extraction on-chip enables a small, portable system that allows for rapid analysis in the field.

Inventors:
; ; ; ;
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1207283
Patent Number(s):
9,096,823
Application Number:
12/872,919
Assignee:
Sandia Corporation (Albuquerque, NM) SSO
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Patent File Date: 2010 Aug 31
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Branch, Darren W., Cooley, Erika Jane, Smith, Gennifer Tanabe, James, Conrad D., and McClain, Jaime L.. Microfluidic device for acoustic cell lysis. United States: N. p., 2015. Web.
Branch, Darren W., Cooley, Erika Jane, Smith, Gennifer Tanabe, James, Conrad D., & McClain, Jaime L.. Microfluidic device for acoustic cell lysis. United States.
Branch, Darren W., Cooley, Erika Jane, Smith, Gennifer Tanabe, James, Conrad D., and McClain, Jaime L.. Tue . "Microfluidic device for acoustic cell lysis". United States. doi:. https://www.osti.gov/servlets/purl/1207283.
@article{osti_1207283,
title = {Microfluidic device for acoustic cell lysis},
author = {Branch, Darren W. and Cooley, Erika Jane and Smith, Gennifer Tanabe and James, Conrad D. and McClain, Jaime L.},
abstractNote = {A microfluidic acoustic-based cell lysing device that can be integrated with on-chip nucleic acid extraction. Using a bulk acoustic wave (BAW) transducer array, acoustic waves can be coupled into microfluidic cartridges resulting in the lysis of cells contained therein by localized acoustic pressure. Cellular materials can then be extracted from the lysed cells. For example, nucleic acids can be extracted from the lysate using silica-based sol-gel filled microchannels, nucleic acid binding magnetic beads, or Nafion-coated electrodes. Integration of cell lysis and nucleic acid extraction on-chip enables a small, portable system that allows for rapid analysis in the field.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Aug 04 00:00:00 EDT 2015},
month = {Tue Aug 04 00:00:00 EDT 2015}
}

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Works referenced in this record:

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journal, September 2005

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Nucleic acid purification using microfabricated silicon structures
journal, October 2003


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