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Genome sequence of Ensifer medicae strain WSM1115; an acid-tolerant Medicago-nodulating microsymbiont from Samothraki, Greece

Journal Article · · Standards in Genomic Sciences
DOI:https://doi.org/10.4056/sigs.4938652· OSTI ID:1628689
 [1];  [2];  [3];  [2];  [3];  [4];  [5];  [5];  [5];  [5];  [6];  [6];  [7];  [6];  [7];  [6];  [6];  [6]
  1. Murdoch Univ., WA (Australia). Centre for Rhizobium Studies; DOE/OSTI
  2. South Australian Research and Development Inst., Urrbrae, SA (Australia)
  3. Murdoch Univ., WA (Australia). Centre for Rhizobium Studies
  4. Deakin Univ., Victoria (Australia). School of Life and Environmental Sciences
  5. Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Bioscience Division
  6. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  7. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biologival Data Mangement and Technology Center
+ Show Author Affiliations
Ensifer medicae strain WSM1115 forms effective nitrogen fixing symbioses with a range of annual Medicago species and is used in commercial inoculants in Australia. WSM1115 is an aerobic, motile, Gram-negative, non-spore-forming rod. It was isolated from a nodule recovered from the root of burr medic (Medicago polymorpha) collected on the Greek Island of Samothraki. WSM1115 has a broad host range for nodulation and N2 fixation capacity within the genus Medicago, although this does not extend to all medic species. WSM1115 is considered saprophytically competent in moderately acid soils (pH(CaCl2 ) 5.0), but it has failed to persist at field sites where soil salinity exceeded 10 ECe (dS/m). Here we describe the features of E. medicae strain WSM1115, together with genome sequence information and its annotation. The 6,861,065 bp high-quality-draft genome is arranged into 7 scaffolds of 28 contigs, contains 6,789 protein-coding genes and 83 RNA-only encoding genes, and is one of 100 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.
Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
AC02-05CH11231; AC52-06NA25396; AC52-07NA27344
OSTI ID:
1628689
Journal Information:
Standards in Genomic Sciences, Journal Name: Standards in Genomic Sciences Journal Issue: 3 Vol. 9; ISSN 1944-3277
Publisher:
BioMed CentralCopyright Statement
Country of Publication:
United States
Language:
English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Alphaproteobacteria
Genetics & Heredity
Microbiology
nitrogen fixation
rhizobia
root-nodule bacteria