Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Rowe, Aaron A.; White, Ryan J.; Bonham, Andrew J.; Plaxco, Kevin W.

doi:10.3791/2922

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Journal Article · Wed Jun 01 00:00:00 EDT 2011 · Journal of Visualized Experiments

DOI:https://doi.org/10.3791/2922· OSTI ID:1628628

Rowe, Aaron A. ^[1]; White, Ryan J. ^[2]; Bonham, Andrew J. ^[2]; Plaxco, Kevin W. ^[3]

Univ. of California, Santa Barbara, CA (United States). Dept. of Chemistry and Biochemistry; DOE/OSTI
Univ. of California, Santa Barbara, CA (United States). Dept. of Chemistry and Biochemistry
Univ. of California, Santa Barbara, CA (United States). Dept. of Chemistry and Biochemistry. Program in BioMolecular Science and Engineering

As medicine is currently practiced, doctors send specimens to a central laboratory for testing and thus must wait hours or days to receive the results. Many patients would be better served by rapid, bedside tests. To this end our laboratory and others have developed a versatile, reagentless biosensor platform that supports the quantitative, reagentless, electrochemical detection of nucleic acids (DNA, RNA), proteins (including antibodies) and small molecules analytes directly in unprocessed clinical and environmental samples. In this video, we demonstrate the preparation and use of several biosensors in this "E-DNA" class. In particular, we fabricate and demonstrate sensors for the detection of a target DNA sequence in a polymerase chain reaction mixture, an HIV-specific antibody and the drug cocaine. The preparation procedure requires only three hours of hands-on effort followed by an overnight incubation, and their use requires only minutes.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC); Bill and Melinda Gates Foundation; National Institutes of Health (NIH)

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 1628628

Journal Information:: Journal of Visualized Experiments, Journal Name: Journal of Visualized Experiments Journal Issue: 52; ISSN 1940-087X; ISSN JVEOA4

Publisher:: MyJoVE Corp.Copyright Statement

Country of Publication:: United States

Language:: English

References (18)

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Cited By (2)

Opportunities, Challenges, and Prospects in Electrochemical Biosensing of Circulating Tumor DNA and its Specific Features No authors listed Sensors, Vol. 19, Issue 17 https://doi.org/10.3390/s19173762	journal	August 2019
Electrochemical Aptamer-Based Sensors for Rapid Point-of-Use Monitoring of the Mycotoxin Ochratoxin A Directly in a Food Stream Somerson, Jacob; Plaxco, Kevin Molecules, Vol. 23, Issue 4 https://doi.org/10.3390/molecules23040912	journal	April 2018

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Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

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