An Adaptable, Portable Microarray Reader for Biodetection

Thompson, Deanna; Pearson, Francesca; Thomas, Cynthia; Rao, Rupa; Matthews, Dennis; Albala, Joanna; Wachsmann-Hogiu, Sebastian; Coleman, Matthew

doi:10.3390/s90402524

An Adaptable, Portable Microarray Reader for Biodetection

Journal Article · Tue Apr 14 00:00:00 EDT 2009 · Sensors

DOI:https://doi.org/10.3390/s90402524· OSTI ID:1628570

Thompson, Deanna ^[1]; Pearson, Francesca ^[2]; Thomas, Cynthia ^[2]; Rao, Rupa ^[3]; Matthews, Dennis ^[4]; Albala, Joanna ^[3]; Wachsmann-Hogiu, Sebastian ^[5]; Coleman, Matthew ^[6]

Univ. of California, Davis, CA (United States). NSF--Center for Biophotonics Science and Technology; DOE/OSTI
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Univ. of California, Davis, CA (United States). NSF--Center for Biophotonics Science and Technology
Univ. of California, Davis, CA (United States). NSF--Center for Biophotonics Science and Technology; Univ. of California, Davis, CA (United States). Dept. of Neurological Surgery
Univ. of California, Davis, CA (United States). NSF--Center for Biophotonics Science and Technology; Univ. of California, Davis, CA (United States). Medical Center. Dept. of Pathology and Lab. Medicine
Univ. of California, Davis, CA (United States). NSF--Center for Biophotonics Science and Technology; Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Univ. of California, Davis, CA (United States). Medical Center. Dept. of Radiation Oncology

We have developed an inexpensive portable microarray reader that can be applied to standard microscope slide-based arrays and other array formats printed on chemically modified surfaces. Measuring only 19 cm in length, the imaging device is portable and may be applicable to both triage and clinical settings. For multiplexing and adaptability purposes, it can be modified to work with multiple excitation/emission wavelengths. Our device is shown to be comparable to a commercial laser scanner when detecting both streptavidin-biotin and antibody interactions. This paper presents the development and characterization of a handheld microarray imager and directly compares it with a commercial scanner.

Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division; National Institutes of Health (NIH); National Science Foundation (NSF)

Grant/Contract Number:: AC52-07NA27344; W-7405-ENG-48

OSTI ID:: 1628570

Journal Information:: Sensors, Journal Name: Sensors Journal Issue: 4 Vol. 9; ISSN SENSC9; ISSN 1424-8220

Publisher:: MDPI AGCopyright Statement

Country of Publication:: United States

Language:: English

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Biosensors for the Detection of Food Pathogens Poltronieri, Palmiro; Mezzolla, Valeria; Primiceri, Elisabetta Foods, Vol. 3, Issue 3 https://doi.org/10.3390/foods3030511	journal	September 2014
Protein Chips for Detection of Salmonella spp. from Enrichment Culture Poltronieri, Palmiro; Cimaglia, Fabio; De Lorenzis, Enrico Sensors, Vol. 16, Issue 4 https://doi.org/10.3390/s16040574	journal	April 2016

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
42 ENGINEERING
47 OTHER INSTRUMENTATION
Chemistry
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immunoassays
point-of-care diagnostics
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protein arrays

An Adaptable, Portable Microarray Reader for Biodetection

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References (13)

Cited By (2)

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