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Enhanced Silver Nanoparticle Synthesis by Escherichia Coli Transformed with Candida Albicans Metallothionein Gene

Journal Article · · Materials
DOI:https://doi.org/10.3390/ma12244180· OSTI ID:1628414
 [1];  [2];  [3]
  1. Alabama A&M Univ., Normal, AL (United States). Dept. of Biological and Environmental Science; DOE/OSTI
  2. Alabama A&M Univ., Normal, AL (United States). Dept. of Biological and Environmental Science
  3. Alabama A&M Univ., Normal, AL (United States). Dept. of Electrical Engineering and Computer Science
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In this study, the metallothionein gene of Candida albicans (C. albicans) was assembled by polymerase chain reaction (PCR), inserted into pUC19 vector, and further transformed into Escherichia coli (E. coli) DH5α cells. The capacity of these recombinant E. coli DH5α cells to synthesize silver nanoparticles was examined. Our results demonstrated that the expression of C. albicans metallothionein in E. coli promoted the bacterial tolerance to metal ions and increased yield of silver nanoparticle synthesis. The compositional and morphological analysis of the silver nanoparticles revealed that silver nanoparticles synthesized by the engineered E. coli cells are around 20 nm in size, and spherical in shape. Importantly, the silver nanoparticles produced by the engineered cells were more homogeneous in shape and size than those produced by bacteria lack of the C. albicans metallothionein. Our study provided preliminary information for further development of the engineered E. coli as a platform for large-scale production of uniform nanoparticles for various applications in nanotechnology.
Research Organization:
Brookhaven National Laboratory (BNL), Upton, NY (United States). Center for Functional Nanomaterials
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities Division
Grant/Contract Number:
SC0012704
OSTI ID:
1628414
Journal Information:
Materials, Journal Name: Materials Journal Issue: 24 Vol. 12; ISSN 1996-1944
Publisher:
MDPICopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (1)

Bacteria Exposed to Silver Nanoparticles Synthesized by Laser Ablation in Water: Modelling E. coli Growth and Inactivation journal February 2020

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36 MATERIALS SCIENCE
Materials Science