Lack of Genomic Instability in Bone Marrow Cells of SCID Mice Exposed Whole-Body to Low-Dose Radiation

Rithidech, Kanokporn; Udomtanakunchai, Chatchanok; Honikel, Louise; Whorton, Elbert

doi:10.3390/ijerph10041356

Lack of Genomic Instability in Bone Marrow Cells of SCID Mice Exposed Whole-Body to Low-Dose Radiation

Journal Article · Mon Apr 01 00:00:00 EDT 2013 · International Journal of Environmental Research and Public Health

DOI:https://doi.org/10.3390/ijerph10041356· OSTI ID:1628327

Rithidech, Kanokporn ^[1]; Udomtanakunchai, Chatchanok ^[2]; Honikel, Louise ^[3]; Whorton, Elbert ^[4]

Stony Brook Univ., NY (United States). Pathology Dept.; DOE/OSTI
Chiang Mai Univ. (Thailand). Center for Excellence for Molecular Imaging. Faculty of Associated Medical Sciences. Dept. of Radiologic Technology
Stony Brook Univ., NY (United States). Pathology Dept.
Texas A & M Univ. at Galveston, TX (United States). Galveston National Lab. Inst. of Human Infections and Immunology

It is clear that high-dose radiation is harmful. However, despite extensive research, assessment of potential health-risks associated with exposure to low-dose radiation (at doses below or equal to 0.1 Gy) is still challenging. Recently, we reported that 0.05 Gy of 137Cs gamma rays (the existing limit for radiation-exposure in the workplace) was incapable of inducing significant in vivo genomic instability (measured by the presence of late-occurring chromosomal damage at 6 months post-irradiation) in bone marrow (BM) cells of two mouse strains, one with constitutively high and one with intermediate levels of the repair enzyme DNA-dependent protein-kinase catalytic-subunit (DNA-PKcs). In this study, we present evidence for a lack of genomic instability in BM cells of the severely combined-immunodeficiency (SCID/J) mouse (which has an extremely low-level of DNA-PKcs activity) exposed whole-body to low-dose radiation (0.05 Gy). Together with our previous report, the data indicate that low-dose radiation (0.05 Gy) is incapable of inducing genomic instability in vivo (regardless of the levels of DNA-PKcs activity of the exposed mice), yet higher doses of radiation (0.1 and 1 Gy) do induce genomic instability in mice with intermediate and extremely low-levels of DNA-PKcs activity (indicating an important role of DNA-PKcs in DNA repair).

View Accepted Manuscript (DOE)

Research Organization:: State Univ. of New York (SUNY), Albany, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: FG02-02ER63311

OSTI ID:: 1628327

Journal Information:: International Journal of Environmental Research and Public Health, Journal Name: International Journal of Environmental Research and Public Health Journal Issue: 4 Vol. 10; ISSN IJERGQ; ISSN 1660-4601

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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No Evidence for the in vivo Induction of Genomic Instability by Low Doses of ¹³⁷ Cs Gamma Rays in Bone Marrow Cells of BALB/cJ and C57BL/6J Mice Rithidech, Kanokporn Noy; Udomtanakunchai, Chatchanok; Honikel, Louise M. Dose-Response, Vol. 10, Issue 1 https://doi.org/10.2203/dose-response.11-002.Rithidech	journal	August 2011

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Late Effects of Low-Dose Radiation on the Bone Marrow, Lung, and Testis Collected From the Same Exposed BALB/cJ Mice Jangiam, Witawat; Udomtanakunchai, Chatchanok; Reungpatthanaphong, Paiboon Dose-Response, Vol. 16, Issue 4 https://doi.org/10.1177/1559325818815031	journal	October 2018
Response to Baverstock, K. Comments on Rithidech, K.N.; et al. Lack of Genomic Instability in Bone Marrow Cells of SCID Mice Exposed Whole-Body to Low-Dose Radiation. Int. J. Environ. Res. Public Health 2013, 10, 1356–1377. Rithidech, Kanokporn; Udomtanakunchai, Chatchanok; Honikel, Louise International Journal of Environmental Research and Public Health, Vol. 10, Issue 7 https://doi.org/10.3390/ijerph10072735	journal	July 2013
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Lack of Genomic Instability in Bone Marrow Cells of SCID Mice Exposed Whole-Body to Low-Dose Radiation

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