Microbial Community Responses to Organophosphate Substrate Additions in Contaminated Subsurface Sediments

Martinez, Robert J.; Wu, Cindy H.; Beazley, Melanie J.; Andersen, Gary L.; Conrad, Mark E.; Hazen, Terry C.; Taillefert, Martial; Sobecky, Patricia A.

doi:10.1371/journal.pone.0100383

Microbial Community Responses to Organophosphate Substrate Additions in Contaminated Subsurface Sediments

Journal Article · Fri Jun 20 00:00:00 EDT 2014 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0100383· OSTI ID:1627707

Martinez, Robert J. ^[1]; Wu, Cindy H. ^[2]; Beazley, Melanie J. ^[3]; Andersen, Gary L. ^[2]; Conrad, Mark E. ^[2]; Hazen, Terry C. ^[4]; Taillefert, Martial ^[5]; Sobecky, Patricia A. ^[3]

Univ. of Alabama, Tuscaloosa, AL (United States). Dept. of Biological Sciences; DOE/OSTI
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Earth Sciences Division
Univ. of Alabama, Tuscaloosa, AL (United States). Dept. of Biological Sciences
Univ. of Tennessee, Knoxville, TN (United States). Dept. of Civil and Environmentla Engineering
Georgia Inst. of Technology, Atlanta, GA (United States). School of Earth and Atmospheric Sciences

Background: Radionuclide- and heavy metal-contaminated subsurface sediments remain a legacy of Cold War nuclear weapons research and recent nuclear power plant failures. Within such contaminated sediments, remediation activities are necessary to mitigate groundwater contamination. A promising approach makes use of extant microbial communities capable of hydrolyzing organophosphate substrates to promote mineralization of soluble contaminants within deep subsurface environments. Methodology/Principal Findings: Uranium-contaminated sediments from the U.S. Department of Energy Oak Ridge Field Research Center (ORFRC) Area 2 site were used in slurry experiments to identify microbial communities involved in hydrolysis of 10 mM organophosphate amendments [i.e., glycerol-2-phosphate (G2P) or glycerol-3-phosphate (G3P)] in synthetic groundwater at pH 5.5 and pH 6.8. Following 36 day (G2P) and 20 day (G3P) amended treatments, maximum phosphate (PO4 32) concentrations of 4.8 mM and 8.9 mM were measured, respectively. Use of the PhyloChip 16S rRNA microarray identified 2,120 archaeal and bacterial taxa representing 46 phyla, 66 classes, 110 orders, and 186 families among all treatments. Measures of archaeal and bacterial richness were lowest under G2P (pH 5.5) treatments and greatest with G3P (pH 6.8) treatments. Members of the phyla Crenarchaeota, Euryarchaeota, Bacteroidetes, and Proteobacteria demonstrated the greatest enrichment in response to organophosphate amendments and the OTUs that increased in relative abundance by 2-fold or greater accounted for 9%–50% and 3%–17% of total detected Archaea and Bacteria, respectively. Conclusions/Significance: This work provided a characterization of the distinct ORFRC subsurface microbial communities that contributed to increased concentrations of extracellular phosphate via hydrolysis of organophosphate substrate amendments. Within subsurface environments that are not ideal for reductive precipitation of uranium, strategies that harness microbial phosphate metabolism to promote uranium phosphate precipitation could offer an alternative approach for in situ sequestration.

View Accepted Manuscript (DOE)

Research Organization:: Georgia Inst. of Technology, Atlanta, GA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Earth and Environmental Systems Science Division

Grant/Contract Number:: AC02-05CH11231; FG02-04ER63906; SC0002530

OSTI ID:: 1627707

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 6 Vol. 9; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Physiological and Comparative Genomic Analysis of Arthrobacter sp. SRS-W-1-2016 Provides Insights on Niche Adaptation for Survival in Uraniferous Soils Chauhan, Ashvini; Pathak, Ashish; Jaswal, Rajneesh Genes, Vol. 9, Issue 1 https://doi.org/10.3390/genes9010031	journal	January 2018
Interactive impacts of earthworms (Eisenia fetida) and arbuscular mycorrhizal fungi (Funneliformis mosseae) on the bioavailability of calcium phosphates Cao, Jia; Wang, Chong; Huang, Yan Plant and Soil, Vol. 396, Issue 1-2 https://doi.org/10.1007/s11104-015-2588-0	journal	July 2015
Use of in-field bioreactors demonstrate groundwater filtration influences planktonic bacterial community assembly, but not biofilm composition Christensen, Geoff A.; Moon, JiWon; Veach, Allison M. PLOS ONE, Vol. 13, Issue 3 https://doi.org/10.1371/journal.pone.0194663	journal	March 2018
Rapid Hydrolysis of Organophosphates Induced by U(IV) Nanoparticles: A Kinetic and Mechanistic Study using Spectroscopic Analysis Cho, Hyejin; Cha, Wansik Colloids and Interfaces, Vol. 3, Issue 4 https://doi.org/10.3390/colloids3040063	journal	November 2019

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