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Title: Uranium Biomineralization by Natural Microbial Phosphatase Activities in the Subsurface

Abstract

In this project, inter-disciplinary research activities were conducted in collaboration among investigators at The University of Alabama (UA), Georgia Institute of Technology (GT), Lawrence Berkeley National Laboratory (LBNL), Brookhaven National Laboratory (BNL), the DOE Joint Genome Institute (JGI), and the Stanford Synchrotron Radiation Light source (SSRL) to: (i) confirm that phosphatase activities of subsurface bacteria in Area 2 and 3 from the Oak Ridge Field Research Center result in solid U-phosphate precipitation in aerobic and anaerobic conditions; (ii) investigate the eventual competition between uranium biomineralization via U-phosphate precipitation and uranium bioreduction; (iii) determine subsurface microbial community structure changes of Area 2 soils following organophosphate amendments; (iv) obtain the complete genome sequences of the Rahnella sp. Y9-602 and the type-strain Rahnella aquatilis ATCC 33071 isolated from these soils; (v) determine if polyphosphate accumulation and phytate hydrolysis can be used to promote U(VI) biomineralization in subsurface sediments; (vi) characterize the effect of uranium on phytate hydrolysis by a new microorganism isolated from uranium-contaminated sediments; (vii) utilize positron-emission tomography to label and track metabolically-active bacteria in soil columns, and (viii) study the stability of the uranium phosphate mineral product. Microarray analyses and mineral precipitation characterizations were conducted in collaboration with DOE SBR-funded investigatorsmore » at LBNL. Thus, microbial phosphorus metabolism has been shown to have a contributing role to uranium immobilization in the subsurface.« less

Authors:
 [1]
  1. Univ. of Alabama, Tuscaloosa, AL (United States)
Publication Date:
Research Org.:
Univ. of Alabama, Tuscaloosa, AL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Contributing Org.:
Georgia Institute of Technology, Atlanta, GA (United States)
OSTI Identifier:
1177452
Report Number(s):
DOE-Alabama-SC0002177
TRN: US1500812
DOE Contract Number:  
SC0002177
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; URANIUM; URANIUM PHOSPHATES; BACTERIA; HYDROLYSIS; POSITRON COMPUTED TOMOGRAPHY; PRECIPITATION; ORNL; PHOSPHORUS; SOILS; SEDIMENTS; ANAEROBIC CONDITIONS; AEROBIC CONDITIONS; REDUCTION; UNDERGROUND; COMMUNITIES; COMPETITION; METABOLISM; SOLIDS; STABILITY; BIOREMEDIATION; MINERALIZATION; PHOSPHATASES; COORDINATED RESEARCH PROGRAMS; CONTAMINATION; bioremediation, uranium biomineralization, phosphorus cycle

Citation Formats

Sobecky, Patricia A. Uranium Biomineralization by Natural Microbial Phosphatase Activities in the Subsurface. United States: N. p., 2015. Web. doi:10.2172/1177452.
Sobecky, Patricia A. Uranium Biomineralization by Natural Microbial Phosphatase Activities in the Subsurface. United States. doi:10.2172/1177452.
Sobecky, Patricia A. Mon . "Uranium Biomineralization by Natural Microbial Phosphatase Activities in the Subsurface". United States. doi:10.2172/1177452. https://www.osti.gov/servlets/purl/1177452.
@article{osti_1177452,
title = {Uranium Biomineralization by Natural Microbial Phosphatase Activities in the Subsurface},
author = {Sobecky, Patricia A.},
abstractNote = {In this project, inter-disciplinary research activities were conducted in collaboration among investigators at The University of Alabama (UA), Georgia Institute of Technology (GT), Lawrence Berkeley National Laboratory (LBNL), Brookhaven National Laboratory (BNL), the DOE Joint Genome Institute (JGI), and the Stanford Synchrotron Radiation Light source (SSRL) to: (i) confirm that phosphatase activities of subsurface bacteria in Area 2 and 3 from the Oak Ridge Field Research Center result in solid U-phosphate precipitation in aerobic and anaerobic conditions; (ii) investigate the eventual competition between uranium biomineralization via U-phosphate precipitation and uranium bioreduction; (iii) determine subsurface microbial community structure changes of Area 2 soils following organophosphate amendments; (iv) obtain the complete genome sequences of the Rahnella sp. Y9-602 and the type-strain Rahnella aquatilis ATCC 33071 isolated from these soils; (v) determine if polyphosphate accumulation and phytate hydrolysis can be used to promote U(VI) biomineralization in subsurface sediments; (vi) characterize the effect of uranium on phytate hydrolysis by a new microorganism isolated from uranium-contaminated sediments; (vii) utilize positron-emission tomography to label and track metabolically-active bacteria in soil columns, and (viii) study the stability of the uranium phosphate mineral product. Microarray analyses and mineral precipitation characterizations were conducted in collaboration with DOE SBR-funded investigators at LBNL. Thus, microbial phosphorus metabolism has been shown to have a contributing role to uranium immobilization in the subsurface.},
doi = {10.2172/1177452},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {4}
}