Use of a Capture-Based Pathogen Transcript Enrichment Strategy for RNA-Seq Analysis of the Francisella Tularensis LVS Transcriptome during Infection of Murine Macrophages

Bent, Zachary W.; Brazel, David M.; Tran-Gyamfi, Mary B.; Hamblin, Rachelle Y.; VanderNoot, Victoria A.; Branda, Steven S.

doi:10.1371/journal.pone.0077834

Use of a Capture-Based Pathogen Transcript Enrichment Strategy for RNA-Seq Analysis of the Francisella Tularensis LVS Transcriptome during Infection of Murine Macrophages

Journal Article · Mon Oct 14 00:00:00 EDT 2013 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0077834· OSTI ID:1627647

Bent, Zachary W. ^[1]; Brazel, David M. ^[2]; Tran-Gyamfi, Mary B. ^[2]; Hamblin, Rachelle Y. ^[2]; VanderNoot, Victoria A. ^[2]; Branda, Steven S. ^[2]

Sandia National Lab. (SNL-CA), Livermore, CA (United States); DOE/OSTI
Sandia National Lab. (SNL-CA), Livermore, CA (United States)

Francisella tularensis is a zoonotic intracellular pathogen that is capable of causing potentially fatal human infections. Like all successful bacterial pathogens, F. tularensis rapidly responds to changes in its environment during infection of host cells, and upon encountering different microenvironments within those cells. This ability to appropriately respond to the challenges of infection requires rapid and global shifts in gene expression patterns. In this study, we use a novel pathogen transcript enrichment strategy and whole transcriptome sequencing (RNA-Seq) to perform a detailed characterization of the rapid and global shifts in F. tularensis LVS gene expression during infection of murine macrophages. We performed differential gene expression analysis on all bacterial genes at two key stages of infection: phagosomal escape, and cytosolic replication. By comparing the F. tularensis transcriptome at these two stages of infection to that of the bacteria grown in culture, we were able to identify sets of genes that are differentially expressed over the course of infection. This analysis revealed the temporally dynamic expression of a number of known and putative transcriptional regulators and virulence factors, providing insight into their role during infection. In addition, we identified several F. tularensis genes that are significantly up-regulated during infection but had not been previously identified as virulence factors. These unknown genes may make attractive therapeutic or vaccine targets.

View Accepted Manuscript (DOE)

Research Organization:: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC04-94AL85000

OSTI ID:: 1627647

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 10 Vol. 8; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Differential Growth of Francisella tularensis, Which Alters Expression of Virulence Factors, Dominant Antigens, and Surface-Carbohydrate Synthases, Governs the Apparent Virulence of Ft SchuS4 to Immunized Animals Holland, Kristen M.; Rosa, Sarah J.; Kristjansdottir, Kolbrun Frontiers in Microbiology, Vol. 8 https://doi.org/10.3389/fmicb.2017.01158	journal	June 2017
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Study of the structure and function of a novel bacterial virulence factor isolated from Francisella tularensis

Technical Report · Wed Aug 08 00:00:00 EDT 2012 · OSTI ID:1079665

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Use of a Capture-Based Pathogen Transcript Enrichment Strategy for RNA-Seq Analysis of the Francisella Tularensis LVS Transcriptome during Infection of Murine Macrophages

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