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The Influence of pCO2 and Temperature on Gene Expression of Carbon and Nitrogen Pathways in Trichodesmium IMS101

Journal Article · · PLoS ONE
 [1];  [2];  [2];  [3]
  1. Bar Ilan Univ., Ramat Gan (Israel). The Mina and Everard Goodman Faculty of Life Sciences; DOE/OSTI
  2. Univ. of Kiel (Germany). Leibniz Inst. of Marine Sciences (IFM-GEOMAR)
  3. Bar Ilan Univ., Ramat Gan (Israel). The Mina and Everard Goodman Faculty of Life Sciences
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Growth, protein amount, and activity levels of metabolic pathways in Trichodesmium are influenced by environmental changes such as elevated pCO2 and temperature. This study examines changes in the expression of essential metabolic genes in Trichodesmium grown under a matrix of pCO2 (400 and 900 matm) and temperature (25 and 31uC). Using RT-qPCR, we studied 21 genes related to four metabolic functional groups: CO2 concentrating mechanism (bicA1, bicA2, ccmM, ccmK2, ccmK3, ndhF4, ndhD4, ndhL, chpX), energy metabolism (atpB, sod, prx, glcD), nitrogen metabolism (glnA, hetR, nifH), and inorganic carbon fixation and photosynthesis (rbcL, rca, psaB, psaC, psbA). nifH and most photosynthetic genes exhibited relatively high abundance and their expression was influenced by both environmental parameters. A two to three orders of magnitude increase was observed for glnA and hetR only when both pCO2 and temperature were elevated. CO2 concentrating mechanism genes were not affected by pCO2 and temperature and their expression levels were markedly lower than that of the nitrogen metabolism and photosynthetic genes. Many of the CO2 concentrating mechanism genes were co-expressed throughout the day. Our results demonstrate that in Trichodesmium, CO2 concentrating mechanism genes are constitutively expressed. Co-expression of genes from different functional groups were frequently observed during the first half of the photoperiod when oxygenic photosynthesis and N2 fixation take place, pointing at the tight and complex regulation of gene expression in Trichodesmium. Here we provide new data linking environmental changes of pCO2 and temperature to gene expression in Trichodesmium. Although gene expression indicates an active metabolic pathway, there is often an uncoupling between transcription and enzyme activity, such that transcript level cannot usually be directly extrapolated to metabolic activity.
Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1627437
Journal Information:
PLoS ONE, Journal Name: PLoS ONE Journal Issue: 12 Vol. 5; ISSN 1932-6203
Publisher:
Public Library of ScienceCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (9)

Reduced nitrogenase efficiency dominates response of the globally important nitrogen fixer Trichodesmium to ocean acidification journal April 2019
CO2 modulation of the rates of photosynthesis and light-dependent O2 consumption in Trichodesmium journal October 2018
An Integrated Response of Trichodesmium erythraeum IMS101 Growth and Photo-Physiology to Iron, CO2, and Light Intensity journal April 2018
Ocean acidification slows nitrogen fixation and growth in the dominant diazotroph Trichodesmium under low-iron conditions journal October 2012
T richodesmium 's strategies to alleviate phosphorus limitation in the future acidified oceans journal March 2014
Trichodesmium – a widespread marine cyanobacterium with unusual nitrogen fixation properties journal May 2013
The complex effects of ocean acidification on the prominent N 2 -fixing cyanobacterium Trichodesmium journal April 2017
First record of a Trichodesmium erythraeum bloom in the Mediterranean Sea 1 This article is derived from a special session entitled “A New Hydrology: Inflow Effects on Ecosystem Form and Functioning” that took place at the February 2011 ASLO Aquatic Sciences conference in San Juan, Puerto Rico. journal August 2012
The physiological cost of diazotrophy for Trichodesmium erythraeum IMS101 journal April 2018

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