CRL2LRR-1 E3-Ligase Regulates Proliferation and Progression through Meiosis in the Caenorhabditis elegans Germline

Burger, Julien; Merlet, Jorge; Tavernier, Nicolas; Richaudeau, Bénédicte; Arnold, Andreas; Ciosk, Rafal; Bowerman, Bruce; Pintard, Lionel

doi:10.1371/journal.pgen.1003375

CRL2LRR-1 E3-Ligase Regulates Proliferation and Progression through Meiosis in the Caenorhabditis elegans Germline

Journal Article · Thu Mar 28 00:00:00 EDT 2013 · PLoS Genetics

DOI:https://doi.org/10.1371/journal.pgen.1003375· OSTI ID:1627299

Burger, Julien ^[1]; Merlet, Jorge ^[2]; Tavernier, Nicolas ^[2]; Richaudeau, Bénédicte ^[2]; Arnold, Andreas ^[3]; Ciosk, Rafal ^[4]; Bowerman, Bruce ^[5]; Pintard, Lionel ^[2]

Universite Paris Diderot, Sorbonne Paris Cite, Paris (France). Centre National de la Recherche Scientifique (CNRS). Institut Jacques Monod; DOE/OSTI
Universite Paris Diderot, Sorbonne Paris Cite, Paris (France). Centre National de la Recherche Scientifique (CNRS). Institut Jacques Monod
Friedrich Miescher Inst. for Biomedical Research, Basel (Switzerland)
Friedrich Miescher Inst. for Biomedical Research, Basel (Switzerland)
Univ. of Oregon, Eugene, OR (United States). Inst. of Molecular Biology

The ubiquitin-proteolytic system controls the stability of proteins in space and time. In this study, using a temperature sensitive mutant allele of the cul-2 gene, we show that CRL2^LRR-1 (CUL-2 RING E3 ubiquitin-ligase and the Leucine Rich Repeat 1 substrate recognition subunit) acts at multiple levels to control germline development. CRL2^LRR-1 promotes germ cell proliferation by counteracting the DNA replication ATL-1 checkpoint pathway. CRL2^LRR-1 also participates in the mitotic proliferation/meiotic entry decision, presumably controlling the stability of meiotic promoting factors in the mitotic zone of the germline. Finally, CRL2^LRR-1 inhibits the first steps of meiotic prophase by targeting in mitotic germ cells degradation of the HORMA domain-containing protein HTP-3, required for loading synaptonemal complex components onto meiotic chromosomes. Given its widespread evolutionary conservation, CUL-2 may similarly regulate germline development in other organisms as well.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Oregon, Eugene, OR (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH)

OSTI ID:: 1627299

Journal Information:: PLoS Genetics, Journal Name: PLoS Genetics Journal Issue: 3 Vol. 9; ISSN 1553-7404

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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