Assembly of Drosophila Centromeric Chromatin Proteins during Mitosis

Mellone, Barbara G.; Grive, Kathryn J.; Shteyn, Vladimir; Bowers, Sarion R.; Oderberg, Isaac; Karpen, Gary H.

doi:10.1371/journal.pgen.1002068

Assembly of Drosophila Centromeric Chromatin Proteins during Mitosis

Journal Article · Thu May 12 00:00:00 EDT 2011 · PLoS Genetics

DOI:https://doi.org/10.1371/journal.pgen.1002068· OSTI ID:1627290

Mellone, Barbara G. ^[1]; Grive, Kathryn J. ^[2]; Shteyn, Vladimir ^[3]; Bowers, Sarion R. ^[2]; Oderberg, Isaac ^[3]; Karpen, Gary H. ^[3]

Univ. of Connecticut, Storrs, CT (United States). Dept. of Molecular and Cell Biology; DOE/OSTI
Univ. of Connecticut, Storrs, CT (United States). Dept. of Molecular and Cell Biology
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Dept. of Genome Dynamics; Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology

Semi-conservative segregation of nucleosomes to sister chromatids during DNA replication creates gaps that must be filled by new nucleosome assembly. We analyzed the cell-cycle timing of centromeric chromatin assembly in Drosophila, which contains the H3 variant CID (CENP-A in humans), as well as CENP-C and CAL1, which are required for CID localization. Pulsechase experiments show that CID and CENP-C levels decrease by 50% at each cell division, as predicted for semiconservative segregation and inheritance, whereas CAL1 displays higher turnover. Quench-chase-pulse experiments demonstrate that there is a significant lag between replication and replenishment of centromeric chromatin. Surprisingly, new CID is recruited to centromeres in metaphase, by a mechanism that does not require an intact mitotic spindle, but does require proteasome activity. Interestingly, new CAL1 is recruited to centromeres before CID in prophase. Furthermore, CAL1, but not CENP-C, is found in complex with pre-nucleosomal CID. Finally, CENP-C displays yet a different pattern of incorporation, during both interphase and mitosis. The unusual timing of CID recruitment and unique dynamics of CAL1 identify a distinct centromere assembly pathway in Drosophila and suggest that CAL1 is a key regulator of centromere propagation.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1627290

Journal Information:: PLoS Genetics, Journal Name: PLoS Genetics Journal Issue: 5 Vol. 7; ISSN 1553-7404

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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