Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover

Gunawan, Christa; Xue, Saisi; Pattathil, Sivakumar; da Costa Sousa, Leonardo; Dale, Bruce E.; Balan, Venkatesh

doi:10.1186/s13068-017-0757-5

Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover

Journal Article · Wed Mar 29 00:00:00 EDT 2017 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-017-0757-5· OSTI ID:1626982

Gunawan, Christa ^[1]; Xue, Saisi ^[2]; Pattathil, Sivakumar ^[3]; da Costa Sousa, Leonardo ^[2]; Dale, Bruce E. ^[2]; Balan, Venkatesh ^[2]

Michigan State Univ., East Lansing, MI (United States), Biomass Conversion Research Lab (BCRL), Chemical Engineering and Materials Science; Michigan State Univ., East Lansing, MI (United States), DOE Great Lakes Bioenergy Research Center (GLBRC); DOE/OSTI
Michigan State Univ., East Lansing, MI (United States), Biomass Conversion Research Lab (BCRL), Chemical Engineering and Materials Science; Michigan State Univ., East Lansing, MI (United States), DOE Great Lakes Bioenergy Research Center (GLBRC)
Univ. of Georgia, Athens, GA (United States), Complex Carbohydrate Research Center; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (US).Biosciences Division, BioEnergy Science Center (BESC)

Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments. Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass). Nearly one third (7 out of 22%) of these total unconverted carbohydrates are present in unhydrolyzed solid (UHS) residues. The presence of these unconverted carbohydrates leads to a considerable sugar yield loss, which negatively impacts the overall economics of the biorefinery. Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins. Thus, obtaining information about the most recalcitrant non-cellulosic glycan cross-linkages becomes a key study to rationally improve commercial enzyme cocktails, by supplementing the required enzyme activities for hydrolyzing those unconverted glycans.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725; FC02-07ER64494

OSTI ID:: 1626982

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Journal Issue: 1 Vol. 10; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

References (52)

Economic evaluation of preconcentration in production of ethanol from dilute sugar solutions Zacchi, G.; Axelsson, A. Biotechnology and Bioengineering, Vol. 34, Issue 2 https://doi.org/10.1002/bit.260340211	journal	June 1989
Energy, wealth, and human development: Why and how biomass pretreatment research must improve Dale, Bruce E.; Ong, Rebecca G. Biotechnology Progress, Vol. 28, Issue 4 https://doi.org/10.1002/btpr.1575	journal	July 2012
Cover Picture: Stereo- and Regioselective Phyllobilane Oxidation in Leaf Homogenates of the Peace Lily (Spathiphyllum wallisii): Hypothetical Endogenous Path to Yellow Chlorophyll Catabolites (Chem. Eur. J. 1/2015) Vergeiner, Clemens; Ulrich, Markus; Li, Chengjie Chemistry - A European Journal, Vol. 21, Issue 1 https://doi.org/10.1002/chem.201490218	journal	December 2014
Structural Diversity and Application Potential of Hemicelluloses Ebringerová, Anna Macromolecular Symposia, Vol. 232, Issue 1 https://doi.org/10.1002/masy.200551401	journal	December 2005
Degradation of different [(glucurono)arabino]xylans by a combination of purified xylan-degrading enzymes Kormelink, F. J. M.; Voragen, A. G. J. Applied Microbiology and Biotechnology, Vol. 38, Issue 5 https://doi.org/10.1007/bf00182811	journal	February 1993
Pectin esterification is spatially regulated both within cell walls and between developing tissues of root apices Knox, J. Paul; Linstead, PaulJ.; King, Janet Planta, Vol. 181, Issue 4 https://doi.org/10.1007/bf00193004	journal	July 1990
One-step biotinylation procedure for carbohydrates to study carbohydrate–protein interactions Grün, Christian H.; van Vliet, Sandra J.; Schiphorst, Wietske E. C. M. Analytical Biochemistry, Vol. 354, Issue 1 https://doi.org/10.1016/j.ab.2006.03.055	journal	July 2006
Synergistic action of enzyme preparations towards recalcitrant corn silage polysaccharides Neumüller, Klaus G.; Streekstra, Hugo; Schols, Henk A. Biomass and Bioenergy, Vol. 60 https://doi.org/10.1016/j.biombioe.2013.11.004	journal	January 2014
Mixture optimization of six core glycosyl hydrolases for maximizing saccharification of ammonia fiber expansion (AFEX) pretreated corn stover Gao, Dahai; Chundawat, Shishir P. S.; Krishnan, Chandraraj Bioresource Technology, Vol. 101, Issue 8, p. 2770-2781 https://doi.org/10.1016/j.biortech.2009.10.056	journal	April 2010
Multifaceted characterization of cell wall decomposition products formed during ammonia fiber expansion (AFEX) and dilute acid based pretreatments Chundawat, Shishir P. S.; Vismeh, Ramin; Sharma, Lekh N. Bioresource Technology, Vol. 101, Issue 21 https://doi.org/10.1016/j.biortech.2010.06.027	journal	November 2010
Comparative material balances around pretreatment technologies for the conversion of switchgrass to soluble sugars Garlock, Rebecca J.; Balan, Venkatesh; Dale, Bruce E. Bioresource Technology, Vol. 102, Issue 24 https://doi.org/10.1016/j.biortech.2011.04.002	journal	December 2011
Profiling of soluble neutral oligosaccharides from treated biomass using solid phase extraction and LC–TOF MS Vismeh, Ramin; Humpula, James F.; Chundawat, Shishir P. S. Carbohydrate Polymers, Vol. 94, Issue 2 https://doi.org/10.1016/j.carbpol.2013.02.005	journal	May 2013
Inhibition of cellulase, xylanase and β-glucosidase activities by softwood lignin preparations Berlin, Alex; Balakshin, Mikhail; Gilkes, Neil Journal of Biotechnology, Vol. 125, Issue 2, p. 198-209 https://doi.org/10.1016/j.jbiotec.2006.02.021	journal	September 2006
Analysis of pectic epitopes recognised by hybridoma and phage display monoclonal antibodies using defined oligosaccharides, polysaccharides, and enzymatic degradation Willats, William G. T.; Limberg, Gerrit; Buchholt, Hans Christian Carbohydrate Research, Vol. 327, Issue 3 https://doi.org/10.1016/s0008-6215(00)00039-2	journal	July 2000
Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7 Clausen, Mads H.; Willats, William G. T.; Knox, J. Paul Carbohydrate Research, Vol. 338, Issue 17 https://doi.org/10.1016/s0008-6215(03)00272-6	journal	August 2003
How biotech can transform biofuels Lynd, Lee R.; Laser, Mark S.; Bransby, David Nature Biotechnology, Vol. 26, Issue 2, p. 169-172 https://doi.org/10.1038/nbt0208-169	journal	February 2008
Application of monoclonal antibodies to investigate plant cell wall deconstruction for biofuels production DeMartini, Jaclyn D.; Pattathil, Sivakumar; Avci, Utku Energy & Environmental Science, Vol. 4, Issue 10 https://doi.org/10.1039/c1ee02112e	journal	January 2011
Cellulosic ethanol production from AFEX-treated corn stover using Saccharomyces cerevisiae 424A(LNH-ST) Lau, M. W.; Dale, B. E. Proceedings of the National Academy of Sciences, Vol. 106, Issue 5, p. 1368-1373 https://doi.org/10.1073/pnas.0812364106	journal	January 2009
4-O-methylation of glucuronic acid in Arabidopsis glucuronoxylan is catalyzed by a domain of unknown function family 579 protein Urbanowicz, B. R.; Pena, M. J.; Ratnaparkhe, S. Proceedings of the National Academy of Sciences, Vol. 109, Issue 35 https://doi.org/10.1073/pnas.1208097109	journal	August 2012
Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEX ^TM -pre-treated biomass Pattathil, Sivakumar; Hahn, Michael G.; Dale, Bruce E. Journal of Experimental Botany, Vol. 66, Issue 14 https://doi.org/10.1093/jxb/erv107	journal	April 2015
A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies Pattathil, S.; Avci, U.; Baldwin, D. Plant Physiology, Vol. 153, Issue 2, p. 514-525 https://doi.org/10.1104/pp.109.151985	journal	April 2010
Yield-determining factors in high-solids enzymatic hydrolysis of lignocellulose Kristensen, Jan B.; Felby, Claus; Jørgensen, Henning Biotechnology for Biofuels, Vol. 2, Issue 1 https://doi.org/10.1186/1754-6834-2-11	journal	January 2009
Hemicellulases and auxiliary enzymes for improved conversion of lignocellulosic biomass to monosaccharides Gao, Dahai; Uppugundla, Nirmal; Chundawat, Shishir PS Biotechnology for Biofuels, Vol. 4, Issue 1 https://doi.org/10.1186/1754-6834-4-5	journal	January 2011
Enhanced characteristics of genetically modified switchgrass (Panicum virgatum L.) for high biofuel production Shen, Hui; Poovaiah, Charleson R.; Ziebell, Angela Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-71	journal	January 2013
Comparative analysis of the ability of Clostridium clariflavum strains and Clostridium thermocellumto utilize hemicellulose and unpretreated plant material Izquierdo, Javier A.; Pattathil, Sivakumar; Guseva, Anna Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/s13068-014-0136-4	journal	November 2014
Sugar loss and enzyme inhibition due to oligosaccharide accumulation during high solids-loading enzymatic hydrolysis Xue, Saisi; Uppugundla, Nirmal; Bowman, Michael J. Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0378-9	journal	November 2015
Chemical and Physicochemical Pretreatment of Lignocellulosic Biomass: A Review Brodeur, Gary; Yau, Elizabeth; Badal, Kimberly Enzyme Research, Vol. 2011 https://doi.org/10.4061/2011/787532	journal	January 2011
Designer synthetic media for studying microbial-catalyzed biofuel production Chundawat, Shishir P. S.; Chambliss, Charles Kevin; Lau, Ming W. Rutgers University https://doi.org/10.7282/t39w0hrx	text	January 2015
A comparative study of ethanol production using dilute acid, ionic liquid and AFEX™ pretreated corn stover Simmons, Blake A.; Singh, Seema; Gao, Xiadi Rutgers University https://doi.org/10.7282/t3bv7jv9	text	January 2014
'Cradle-to-grave' assessment of existing lignocellulose pretreatment technologies Sousa, Leonardo; Chundawat, Shishir P. S.; Balan, Venkatesh Rutgers University https://doi.org/10.7282/t3cv4m03	text	January 2009
Automated Synthesis of Arabinoxylan-Oligosaccharides Enables Characterization of Antibodies that Recognize Plant Cell Wall Glycans Schmidt, Deborah; Schuhmacher, Frank; Geissner, Andreas Chemistry - A European Journal, Vol. 21, Issue 15 https://doi.org/10.1002/chem.201500065	journal	February 2015
Lignocellulosic Biomass Pretreatment Using AFEX Balan, Venkatesh; Bals, Bryan; Chundawat, Shishir P. S. Biofuels, p. 61-77 https://doi.org/10.1007/978-1-60761-214-8_5	book	January 2009
Biomass Conversion Decker, Stephen R.; Sheehan, John; Dayton, David C. Handbook of Industrial Chemistry and Biotechnology https://doi.org/10.1007/978-3-319-52287-6_6	book	January 2017
Analysis of pectic epitopes recognised by hybridoma and phage display monoclonal antibodies using defined oligosaccharides, polysaccharides, and enzymatic degradation Willats, William G. T.; Limberg, Gerrit; Buchholt, Hans Christian Carbohydrate Research, Vol. 327, Issue 3 https://doi.org/10.1016/S0008-6215(00)00039-2	journal	July 2000
Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7 Clausen, Mads H.; Willats, William G. T.; Knox, J. Paul Carbohydrate Research, Vol. 338, Issue 17 https://doi.org/10.1016/S0008-6215(03)00272-6	journal	August 2003
Microbial hemicellulases Shallom, Dalia; Shoham, Yuval Current Opinion in Microbiology, Vol. 6, Issue 3, p. 219-228 https://doi.org/10.1016/S1369-5274(03)00056-0	journal	June 2003
‘Cradle-to-grave’ assessment of existing lignocellulose pretreatment technologies da Costa Sousa, Leonardo; Chundawat, Shishir PS; Balan, Venkatesh Current Opinion in Biotechnology, Vol. 20, Issue 3 https://doi.org/10.1016/j.copbio.2009.05.003	journal	June 2009
Techno-economic comparison of process technologies for biochemical ethanol production from corn stover Kazi, Feroz Kabir; Fortman, Joshua A.; Anex, Robert P. Fuel, Vol. 89, p. S20-S28 https://doi.org/10.1016/j.fuel.2010.01.001	journal	November 2010
Bioethanol production from agricultural wastes: An overview Sarkar, Nibedita; Ghosh, Sumanta Kumar; Bannerjee, Satarupa Renewable Energy, Vol. 37, Issue 1 https://doi.org/10.1016/j.renene.2011.06.045	journal	January 2012
Biofuels Done Right: Land Efficient Animal Feeds Enable Large Environmental and Energy Benefits Dale, Bruce E.; Bals, Bryan D.; Kim, Seungdo Environmental Science & Technology, Vol. 44, Issue 22 https://doi.org/10.1021/es101864b	journal	November 2010
Application of monoclonal antibodies to investigate plant cell wall deconstruction for biofuels production DeMartini, Jaclyn D.; Pattathil, Sivakumar; Avci, Utku Energy & Environmental Science, Vol. 4, Issue 10 https://doi.org/10.1039/C1EE02112E	journal	January 2011
Identification of features associated with plant cell wall recalcitrance to pretreatment by alkaline hydrogen peroxide in diverse bioenergy feedstocks using glycome profiling Li, Muyang; Pattathil, Sivakumar; Hahn, Michael G. RSC Adv., Vol. 4, Issue 33 https://doi.org/10.1039/C4RA00824C	journal	January 2014
Next-generation ammonia pretreatment enhances cellulosic biofuel production da Costa Sousa, Leonardo; Jin, Mingjie; Chundawat, Shishir P. S. Energy & Environmental Science, Vol. 9, Issue 4 https://doi.org/10.1039/C5EE03051J	journal	January 2016
Cell wall-associated transition metals improve alkaline-oxidative pretreatment in diverse hardwoods Bansal, Namita; Bhalla, Aditya; Pattathil, Sivakumar Green Chemistry, Vol. 18, Issue 5 https://doi.org/10.1039/C5GC01748C	journal	January 2016
Multi-scale visualization and characterization of lignocellulosic plant cell wall deconstruction during thermochemical pretreatment Chundawat, Shishir P. S.; Donohoe, Bryon S.; da Costa Sousa, Leonardo Energy & Environmental Science, Vol. 4, Issue 3, 973 https://doi.org/10.1039/c0ee00574f	journal	January 2011
Deconstruction of Lignocellulosic Biomass to Fuels and Chemicals Chundawat, Shishir P. S.; Beckham, Gregg T.; Himmel, Michael E. Annual Review of Chemical and Biomolecular Engineering, Vol. 2, Issue 1, p. 121-145 https://doi.org/10.1146/annurev-chembioeng-061010-114205	journal	July 2011
Comparison of enzymatic reactivity of corn stover solids prepared by dilute acid, AFEX™, and ionic liquid pretreatments Gao, Xiadi; Kumar, Rajeev; Singh, Seema Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/1754-6834-7-71	journal	January 2014
A comparative study of ethanol production using dilute acid, ionic liquid and AFEX™ pretreated corn stover Uppugundla, Nirmal; da Costa Sousa, Leonardo; Chundawat, Shishir PS Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/1754-6834-7-72	journal	January 2014
Biomass conversion Wang, Keyi Michigan State University https://doi.org/10.25335/m59p2w88q	text	January 1999
A packed bed Ammonia Fiber Expansion reactor system for pretreatment of agricultural residues at regional depots Campbell, Timothy J.; Teymouri, Farzaneh; Bals, Bryan Biofuels, Vol. 4, Issue 1 https://doi.org/10.4155/bfs.12.71	journal	January 2013
Hemicellulases and auxiliary enzymes for improved conversion of lignocellulosic biomass to monosaccharides Gao, Dahai; Uppugundla, Nirmal; Chundawat, Shishir P. S. Rutgers University https://doi.org/10.7282/t31j9d26	text	January 2011
Multi-scale visualization and characterization of lignocellulosic plant cell wall deconstruction during thermochemical pretreatment Chundawat, Shishir P. S.; Donohoe, Bryon S.; Sousa, Leonardo Rutgers University https://doi.org/10.7282/t3j1062s	text	January 2011

Cited By (2)

Ethanol production potential from AFEX™ and steam-exploded sugarcane residues for sugarcane biorefineries Mokomele, Thapelo; da Costa Sousa, Leonardo; Balan, Venkatesh Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1130-z	journal	May 2018
Cooperative valorization of lignin and residual sugar to polyhydroxyalkanoate (PHA) for enhanced yield and carbon utilization in biorefineries Liu, Zhi-Hua; Shinde, Somnath; Xie, Shangxian Sustainable Energy & Fuels, Vol. 3, Issue 8 https://doi.org/10.1039/c9se00021f	journal	January 2019

Similar Records

Effects of Extractive Ammonia Pretreatment on the Ultrastructure and Glycan Composition of Corn Stover

Journal Article · Sun Sep 15 20:00:00 EDT 2019 · Frontiers in Energy Research · OSTI ID:1609252

Influence of Physico-Chemical Changes on Enzymatic Digestibility of Ionic Liquid and AFEX pretreated Corn Stover

Journal Article · Fri Dec 31 23:00:00 EST 2010 · Journal of the American Chemical Society · OSTI ID:1015020

Understanding the structure and composition of recalcitrant oligosaccharides in hydrolysate using high-throughput biotin-based glycome profiling and mass spectrometry

Journal Article · Mon Feb 14 19:00:00 EST 2022 · Scientific Reports · OSTI ID:1904710

Related Subjects

Biotechnology & Applied Microbiology
Energy & Fuels

of Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover Gunawan, Christa; Xue, Saisi; Pattathil, Sivakumar https://doi.org/10.6084/m9.figshare.10041905 The current record is supplemented by this image	image	January 2019
of Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover Gunawan, Christa; Xue, Saisi; Pattathil, Sivakumar https://doi.org/10.6084/m9.figshare.10041905.v1 The current record is supplemented by this image	image	January 2019
Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover Gunawan, Christa; Xue, Saisi; Pattathil, Sivakumar https://doi.org/10.6084/m9.figshare.c.3729823 The current record is supplemented by this collection	collection	January 2017
Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover Gunawan, Christa; Xue, Saisi; Pattathil, Sivakumar https://doi.org/10.6084/m9.figshare.c.3729823.v1 The current record is supplemented by this collection	collection	January 2017

Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover

Citation Formats

References (52)

Cited By (2)

Linked Research (4)

Similar Records

Related Subjects