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Title: Induction of D-xylose uptake and expression of NAD(P)H-linked xylose reductase and NADP + -linked xylitol dehydrogenase in the oleaginous microalga Chlorella sorokiniana

Abstract

The heterotrophic and mixotrophic culture of oleaginous microalgae is a promising process to produce biofuel feedstock due to the advantage of fast growth. Various organic carbons have been explored for this application. However, despite being one of the most abundant and economical sugar resources in nature, D-xylose has never been demonstrated as a carbon source for wild-type microalgae. The purpose of the present work was to identify the feasibility of D-xylose utilization by the oleaginous microalga Chlorella sorokiniana. The sugar uptake kinetic analysis was performed with 14C-labeled sugars and the data showed that the D-glucose induced algal cells (the alga was heterotrophically grown on D-glucose and then harvested as D-glucose induced cells) exhibited a remarkably increased D-xylose uptake rate. The maximum D-xylose transport rate was 3.8 nmol min -1 mg -1 dry cell weight (DCW) with K m value of 6.8 mM. D-xylose uptake was suppressed in the presence of D-glucose, D-galactose and D-fructose but not L-arabinose and D-ribose. The uptake of D-xylose activated the related metabolic pathway, and the activities of a NAD(P)H-linked xylose reductase (XR) and a unique NADP +-linked xylitol dehydrogenase (XDH) were detected in C. sorokiniana. Compared with the culture in the dark, the consumption ofmore » D-xylose increased 2 fold under light but decreased to the same level with addition of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), indicating that extra chemical energy from the light-dependent reaction contributed the catabolism of D-xylose for C. sorokiniana. An inducible D-xylose transportation system and a related metabolic pathway were discovered for microalga for the first time. The transportation of D-xylose across the cell membrane of C. sorokiniana could be realized by an inducible hexose symporter. The uptake of D-xylose subsequently activated the expression of key catalytic enzymes that enabled D-xylose entering central metabolism. Results of this research are useful to better understand the D-xylose metabolic pathway in the microalga C. sorokiniana and provide a target for genetic engineering to improve D-xylose utilization for microalgal lipid production.« less

Authors:
 [1];  [1];  [1];  [1];  [1]
  1. Washington State Univ., Pullman, WA (United States). Biological Systems Engineering
Publication Date:
Research Org.:
Washington State Univ., Pullman, WA (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1626957
Grant/Contract Number:  
EE0003112
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; xylitol dehydrogenase; xylose reductase; D-xylose; microalgae; Chlorella sorokiniana

Citation Formats

Zheng, Yubin, Yu, Xiaochen, Li, Tingting, Xiong, Xiaochao, and Chen, Shulin. Induction of D-xylose uptake and expression of NAD(P)H-linked xylose reductase and NADP + -linked xylitol dehydrogenase in the oleaginous microalga Chlorella sorokiniana. United States: N. p., 2014. Web. doi:10.1186/s13068-014-0125-7.
Zheng, Yubin, Yu, Xiaochen, Li, Tingting, Xiong, Xiaochao, & Chen, Shulin. Induction of D-xylose uptake and expression of NAD(P)H-linked xylose reductase and NADP + -linked xylitol dehydrogenase in the oleaginous microalga Chlorella sorokiniana. United States. doi:10.1186/s13068-014-0125-7.
Zheng, Yubin, Yu, Xiaochen, Li, Tingting, Xiong, Xiaochao, and Chen, Shulin. Fri . "Induction of D-xylose uptake and expression of NAD(P)H-linked xylose reductase and NADP + -linked xylitol dehydrogenase in the oleaginous microalga Chlorella sorokiniana". United States. doi:10.1186/s13068-014-0125-7. https://www.osti.gov/servlets/purl/1626957.
@article{osti_1626957,
title = {Induction of D-xylose uptake and expression of NAD(P)H-linked xylose reductase and NADP + -linked xylitol dehydrogenase in the oleaginous microalga Chlorella sorokiniana},
author = {Zheng, Yubin and Yu, Xiaochen and Li, Tingting and Xiong, Xiaochao and Chen, Shulin},
abstractNote = {The heterotrophic and mixotrophic culture of oleaginous microalgae is a promising process to produce biofuel feedstock due to the advantage of fast growth. Various organic carbons have been explored for this application. However, despite being one of the most abundant and economical sugar resources in nature, D-xylose has never been demonstrated as a carbon source for wild-type microalgae. The purpose of the present work was to identify the feasibility of D-xylose utilization by the oleaginous microalga Chlorella sorokiniana. The sugar uptake kinetic analysis was performed with 14C-labeled sugars and the data showed that the D-glucose induced algal cells (the alga was heterotrophically grown on D-glucose and then harvested as D-glucose induced cells) exhibited a remarkably increased D-xylose uptake rate. The maximum D-xylose transport rate was 3.8 nmol min-1 mg-1 dry cell weight (DCW) with K m value of 6.8 mM. D-xylose uptake was suppressed in the presence of D-glucose, D-galactose and D-fructose but not L-arabinose and D-ribose. The uptake of D-xylose activated the related metabolic pathway, and the activities of a NAD(P)H-linked xylose reductase (XR) and a unique NADP+-linked xylitol dehydrogenase (XDH) were detected in C. sorokiniana. Compared with the culture in the dark, the consumption of D-xylose increased 2 fold under light but decreased to the same level with addition of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), indicating that extra chemical energy from the light-dependent reaction contributed the catabolism of D-xylose for C. sorokiniana. An inducible D-xylose transportation system and a related metabolic pathway were discovered for microalga for the first time. The transportation of D-xylose across the cell membrane of C. sorokiniana could be realized by an inducible hexose symporter. The uptake of D-xylose subsequently activated the expression of key catalytic enzymes that enabled D-xylose entering central metabolism. Results of this research are useful to better understand the D-xylose metabolic pathway in the microalga C. sorokiniana and provide a target for genetic engineering to improve D-xylose utilization for microalgal lipid production.},
doi = {10.1186/s13068-014-0125-7},
journal = {Biotechnology for Biofuels},
issn = {1754-6834},
number = 1,
volume = 7,
place = {United States},
year = {2014},
month = {10}
}

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Works referenced in this record:

Review on biofuel oil and gas production processes from microalgae
journal, July 2009


Growing Phototrophic Cells without Light
journal, May 2006


Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions
journal, March 2009


Two-stage heterotrophic and phototrophic culture strategy for algal biomass and lipid production
journal, January 2012


A laboratory study of producing docosahexaenoic acid from biodiesel-waste glycerol by microalgal fermentation
journal, November 2007


Biodiesel production from algal oil using cassava (Manihot esculenta Crantz) as feedstock
journal, December 2009


Feasibility of filamentous fungi for biofuel production using hydrolysate from dilute sulfuric acid pretreatment of wheat straw
journal, January 2012

  • Zheng, Yubin; Yu, Xiaochen; Zeng, Jijiao
  • Biotechnology for Biofuels, Vol. 5, Issue 1
  • DOI: 10.1186/1754-6834-5-50

Best practices in heterotrophic high-cell-density microalgal processes: achievements, potential and possible limitations
journal, May 2011

  • Bumbak, Fabian; Cook, Stella; Zachleder, Vilém
  • Applied Microbiology and Biotechnology, Vol. 91, Issue 1
  • DOI: 10.1007/s00253-011-3311-6

Regulation of Hexose Transport in Chlorella vulgaris: Characteristics of Induction and Turnover
journal, January 1974


Heterotrophic cultures of microalgae: Metabolism and potential products
journal, January 2011


Utilization of Xylose for Growth by the Eukaryotic Alga, Chlorella
journal, June 1999


CARBOHYDRATE NUTRITION OF CHLORELLA VULGARIS
journal, January 1951

  • Neish, A. C.
  • Canadian Journal of Botany, Vol. 29, Issue 1
  • DOI: 10.1139/b51-007

On the Heterotrophic Growth of Chlorella pyrenoidosa
journal, February 1958


Xylose as a Specific Inhibitor of Photosynthesis
journal, May 1958


High productivity cultivation of a heat-resistant microalga Chlorella sorokiniana for biofuel production
journal, March 2013


High-density fed-batch culture of a thermotolerant microalga Chlorella sorokiniana for biofuel production
journal, August 2013


Metabolic engineering for improved fermentation of pentoses by yeasts
journal, May 2004


Structure-Guided Engineering of Xylitol Dehydrogenase Cosubstrate Specificity
journal, March 2006


Engineering Redox Cofactor Regeneration for Improved Pentose Fermentation in Saccharomyces cerevisiae
journal, October 2003


Effect of External pH on the Internal pH of Chlorella saccharophila
journal, April 1985

  • Gehl, Katharina A.; Colman, Brian
  • Plant Physiology, Vol. 77, Issue 4
  • DOI: 10.1104/pp.77.4.917

Effects of Environmental pH on the Internal pH of Chlorella pyrenoidosa, Scenedesmus quadricauda, and Euglena mutabilis
journal, August 1981


Xylose fermentation by Saccharomyces cerevisiae
journal, March 1993

  • K�tter, Peter; Ciriacy, Michael
  • Applied Microbiology and Biotechnology, Vol. 38, Issue 6
  • DOI: 10.1007/BF00167144

Discovery and characterization of novel d-xylose-specific transporters from Neurospora crassa and Pichia stipitis
journal, January 2010

  • Du, Jing; Li, Sijin; Zhao, Huimin
  • Molecular BioSystems, Vol. 6, Issue 11
  • DOI: 10.1039/c0mb00007h

A kinetic analysis of d-xylose transport in rhodotorula glutinis
journal, July 1978

  • Alcorn, Mary E.; Griffin, Charles C.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 510, Issue 2
  • DOI: 10.1016/0005-2736(78)90036-6

Oil production by oleaginous yeasts using the hydrolysate from pretreatment of wheat straw with dilute sulfuric acid
journal, May 2011