Widespread signatures of selection for secreted peptidases in a fungal plant pathogen

Krishnan, Parvathy; Ma, Xin; McDonald, Bruce A.; Brunner, Patrick C.

doi:10.1186/s12862-018-1123-3

Widespread signatures of selection for secreted peptidases in a fungal plant pathogen

Journal Article · Wed Jan 24 00:00:00 EST 2018 · BMC Evolutionary Biology (Online)

DOI:https://doi.org/10.1186/s12862-018-1123-3· OSTI ID:1626781

Krishnan, Parvathy ^[1]; Ma, Xin ^[2]; McDonald, Bruce A. ^[2]; Brunner, Patrick C. ^[2]

Eidgenoessische Technische Hochschule, Zurich (Switzerland). Inst. of Integrative Biology. Plant Pathology; DOE/OSTI
Eidgenoessische Technische Hochschule, Zurich (Switzerland). Inst. of Integrative Biology. Plant Pathology

Background: Fungal plant pathogens secrete a large arsenal of hydrolytic enzymes during the course of infection, including peptidases. Secreted peptidases have been extensively studied for their role as effectors. In this study, we combined transcriptomics, comparative genomics and evolutionary analyses to investigate all 39 secreted peptidases in the fungal wheat pathogen Zymoseptoria tritici and its close relatives Z. pseudotritici and Z. ardabiliae. Results: RNA-seq data revealed that a majority of the secreted peptidases displayed differential transcription during the course of Z. tritici infection, indicative of specialization for different stages in the life cycle. Evolutionary analyses detected widespread evidence of adaptive evolution acting on at least 28 of the peptidases. A few peptidases displayed lineage-specific rates of molecular evolution, suggesting altered selection pressure in Z. tritici following host specialization on domesticated wheat. The peptidases belonging to MEROPS families A1 and G1 emerged as a particularly interesting group that may play key roles in host-pathogen co-evolution, host adaptation and pathogenicity. Sister genes in the A1 and G1 families showed accelerated substitution rates after gene duplications. Conclusions: These results suggest widespread evolution of secreted peptidases leading to novel gene functions, consistent with predicted models of “escape from adaptive conflict” and “neo-functionalization”. Our analyses identified candidate genes worthy of functional analyses that may encode effector functions, for example by suppressing plant defenses during the biotrophic phase of infection.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1626781

Journal Information:: BMC Evolutionary Biology (Online), Journal Name: BMC Evolutionary Biology (Online) Journal Issue: 1 Vol. 18; ISSN 1471-2148

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Adaptive evolution
Escape from adaptive conflict (EAC)
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Neo-functionalization (NEO-F)
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Transcriptomics

Widespread signatures of selection for secreted peptidases in a fungal plant pathogen Krishnan, Parvathy; Ma, Xin; McDonald, Bruce https://doi.org/10.6084/M9.FIGSHARE.C.3986367 The current record is supplemented by this collection	collection	January 2018
Additional file 1: of Widespread signatures of selection for secreted peptidases in a fungal plant pathogen Krishnan, Parvathy; Ma, Xin; McDonald, Bruce https://doi.org/10.6084/m9.figshare.5821689 The current record is supplemented by this text	text	January 2018
Additional file 1: of Widespread signatures of selection for secreted peptidases in a fungal plant pathogen Krishnan, Parvathy; Ma, Xin; McDonald, Bruce https://doi.org/10.6084/m9.figshare.5821689.v1 The current record is supplemented by this text	text	January 2018
Widespread signatures of selection for secreted peptidases in a fungal plant pathogen Krishnan, Parvathy; Ma, Xin; McDonald, Bruce https://doi.org/10.6084/m9.figshare.c.3986367.v1 The current record is supplemented by this collection	collection	January 2018

Widespread signatures of selection for secreted peptidases in a fungal plant pathogen

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