Distinct short-range ovule signals attract or repel Arabidopsis thaliana pollen tubes in vitro

Palanivelu, Ravishankar; Preuss, Daphne

doi:10.1186/1471-2229-6-7

Distinct short-range ovule signals attract or repel Arabidopsis thaliana pollen tubes in vitro

Journal Article · Wed Apr 05 00:00:00 EDT 2006 · BMC Plant Biology

DOI:https://doi.org/10.1186/1471-2229-6-7· OSTI ID:1626507

Palanivelu, Ravishankar ^[1]; Preuss, Daphne ^[2]

Univ. of Chicago, IL (United States); DOE/OSTI
Univ. of Chicago, IL (United States)

Pollen tubes deliver sperm after navigating through flower tissues in response to attractive and repulsive cues. Genetic analyses in maize and Arabidopsis thaliana and cell ablation studies in Torenia fournieri have shown that the female gametophyte (the 7-celled haploid embryo sac within an ovule) and surrounding diploid tissues are essential for guiding pollen tubes to ovules. The variety and inaccessibility of these cells and tissues has made it challenging to characterize the sources of guidance signals and the dynamic responses they elicit in the pollen tubes. Here we developed an in vitro assay to study pollen tube guidance to excised A. thaliana ovules. Using this assay we discerned the temporal and spatial regulation and species-specificity of late stage guidance signals and characterized the dynamics of pollen tube responses. We established that unfertilized A. thaliana ovules emit diffusible, developmentally regulated, species-specific attractants, and demonstrated that ovules penetrated by pollen tubes rapidly release diffusible pollen tube repellents. These results demonstrate that in vitro pollen tube guidance to excised A. thaliana ovules efficiently recapitulates much of in vivo pollen tube behaviour during the final stages of pollen tube growth. This assay will aid in confirming the roles of candidate guidance molecules, exploring the phenotypes of A. thaliana pollen tube guidance mutants and characterizing interspecies pollination interactions.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Chicago, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: FG02-96ER20240

OSTI ID:: 1626507

Journal Information:: BMC Plant Biology, Journal Name: BMC Plant Biology Journal Issue: 1 Vol. 6; ISSN 1471-2229

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Female Gametophyte
Guidance Signal
Plant Sciences
Pollen Tube
Pollen Tube Growth
Synergid Cell

Distinct short-range ovule signals attract or repel Arabidopsis thaliana pollen tubes in vitro

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