Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation

Sherrer, R. Lynn; O’Donoghue, Patrick; Söll, Dieter

doi:10.1093/nar/gkm1134

Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation

Journal Article · Thu Jan 03 00:00:00 EST 2008 · Nucleic Acids Research

DOI:https://doi.org/10.1093/nar/gkm1134· OSTI ID:1625422

Sherrer, R. Lynn ^[1]; O’Donoghue, Patrick ^[2]; Söll, Dieter ^[3]

Yale Univ., New Haven, CT (United States). Dept. of Molecular Biophysics. Dept. of Biochemistry; DOE/OSTI
Yale Univ., New Haven, CT (United States). Dept. of Molecular Biophysics. Dept. of Biochemistry
Yale Univ., New Haven, CT (United States). Dept. of Molecular Biophysics. Dept. of Biochemistry. Dept. of Chemistry

Selenocysteine (Sec)-decoding archaea and eukaryotes employ a unique route of Sec-tRNA^Sec synthesis in which O-phosphoseryl-tRNA^Sec kinase (PSTK) phosphorylates Ser-tRNASec to produce the O-phosphoseryl-tRNA^Sec (Sep-tRNA^Sec) substrate that Sep-tRNA:Sec-tRNA synthase (SepSecS) converts to Sec-tRNA^Sec. This study presents a biochemical characterization of Methanocaldococcus jannaschii PSTK, including kinetics of Sep-tRNA^Sec formation (K_m for Ser-tRNA^Sec of 40 nM and ATP of 2.6 mM). PSTK binds both Ser-tRNA^Sec and tRNA^Sec with high affinity (K_d values of 53 nM and 39 nM, respectively). The ATPase activity of PSTK may be activated via an induced fit mechanism in which binding of tRNA^Sec specifically stimulates hydrolysis. Albeit with lower activity than ATP, PSTK utilizes GTP, CTP, UTP and dATP as phosphatedonors. Homology with related kinases allowed prediction of the ATPase active site, comprised of phosphate-binding loop (P-loop), Walker B and RxxxR motifs. Gly14, Lys17, Ser18, Asp41, Arg116 and Arg120 mutations resulted in enzymes with decreased activity highlighting the importance of these conserved motifs in PSTK catalysis both in vivo and in vitro. Phylogenetic analysis of PSTK in the context of its ‘DxTN’ kinase family shows that PSTK co-evolved precisely with SepSecS and indicates the presence of a previously unidentified PSTK in Plasmodium species.

View Accepted Manuscript (DOE)

Research Organization:: Yale Univ., New Haven, CT (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

OSTI ID:: 1625422

Journal Information:: Nucleic Acids Research, Journal Name: Nucleic Acids Research Journal Issue: 4 Vol. 36; ISSN 0305-1048

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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