Compositions and methods for making selenocysteine containing polypeptides

Soll, Dieter; Mukai, Takahito; Hoffman, Kyle

Compositions and methods for making selenocysteine containing polypeptides

Patent · 17 October 2023

OSTI ID:2293725

Soll, Dieter; Mukai, Takahito; Hoffman, Kyle

Non-naturally occurring tRNA^Sec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNA^Sec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNASec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNA^Sec.

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Research Organization:: Yale Univ., New Haven, CT (United States)

Sponsoring Organization:: USDOE; National Institutes of Health (NIH); National Science Foundation (NSF)

DOE Contract Number:: FG02-98ER20311

Assignee:: Yale University (New Haven, CT)

Patent Number(s):: 11,788,111

Application Number:: 16/754,130

OSTI ID:: 2293725

Country of Publication:: United States

Language:: English

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