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Evidence for the involvement of the Arabidopsis SEC24A in male transmission

Journal Article · · Journal of Experimental Botany
DOI:https://doi.org/10.1093/jxb/err174· OSTI ID:1625371
 [1];  [2];  [2];  [2];  [2];  [2];  [2]
  1. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory; DOE/OSTI
  2. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory
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Eukaryotic cells use COPII-coated carriers for endoplasmic reticulum (ER)-to-Golgi protein transport. Selective cargo capture into ER-derived carriers is largely driven by the SEC24 component of the COPII coat. The Arabidopsis genome encodes three AtSEC24 genes with overlapping expression profiles but it is yet to be established whether the AtSEC24 proteins have overlapping roles in plant growth and development. Taking advantage of Arabidopsis thaliana as a model plant system for studying gene function in vivo, through reciprocal crosses, pollen characterization, and complementation tests, evidence is provided for a role for AtSEC24A in the male gametophyte. It is established that an AtSEC24A loss-of-function mutation is tolerated in the female gametophyte but that it causes defects in pollen leading to failure of male transmission of the AtSEC24A mutation. These data provide a characterization of plant SEC24 family in planta showing incompletely overlapping functions of the AtSEC24 isoforms. The results also attribute a novel role to SEC24 proteins in a multicellular model system, specifically in male fertility.

Research Organization:
Michigan State Univ., East Lansing, MI (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
FG02-91ER20021
OSTI ID:
1625371
Journal Information:
Journal of Experimental Botany, Journal Name: Journal of Experimental Botany Journal Issue: 14 Vol. 62; ISSN 0022-0957
Publisher:
Oxford University PressCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (8)

Proteomic analysis of endoplasmic reticulum stress responses in rice seeds journal September 2015
Identification of rice cornichon as a possible cargo receptor for the Golgi-localized sodium transporter OsHKT1;3 journal March 2015
The Arabidopsis COPII components, AtSEC23A and AtSEC23D, are essential for pollen wall development and exine patterning journal January 2018
ER and vacuoles: never been closer journal January 2014
Identification of Novel Components of the Unfolded Protein Response in Arabidopsis journal May 2016
Endoplasmic Reticulum (ER) Stress Response and Its Physiological Roles in Plants journal April 2013
IRE1, a component of the unfolded protein response signaling pathway, protects pollen development in Arabidopsis from heat stress journal August 2016
The ER luminal C‐terminus of AtSec62 is critical for male fertility and plant growth in Arabidopsis thaliana journal September 2019

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Related Subjects

54 ENVIRONMENTAL SCIENCES
59 BASIC BIOLOGICAL SCIENCES
AtSEC24
COPII
Plant Sciences
endoplasmic reticulum
pollen
protein traffic