Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Unfolded Protein Response in Arabidopsis

Journal Article · · Methods in Molecular Biology
 [1];  [2]
  1. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory; MSU DOE Plant Research Laboratory
  2. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory
The unfolded protein response (UPR) is a highly regulated signaling pathway that is largely conserved across eukaryotes. It is essential for cell homeostasis under environmental and physiological conditions that perturb the protein folding in the endoplasmic reticulum (ER). Arabidopsis is one of the outstanding multicellular model systems in which to investigate the UPR. Here, we described a protocol to induce the UPR in plants, specifically arabidopsis, and to estimate their ability to cope with ER stress through the quantification of physiological parameters.
Research Organization:
Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory
Sponsoring Organization:
AgBioResearch; National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division
Grant/Contract Number:
FG02-91ER20021
OSTI ID:
1608628
Journal Information:
Methods in Molecular Biology, Journal Name: Methods in Molecular Biology Vol. 1691; ISSN 1064-3745
Publisher:
SpringerCopyright Statement
Country of Publication:
United States
Language:
English

References (14)

Consistent Sets of Spectrophotometric Chlorophyll Equations for Acetone, Methanol and Ethanol Solvents journal June 2006
Unfolded protein response in plants: one master, many questions journal October 2015
IRE1: ER stress sensor and cell fate executor journal November 2013
Chlorophyll and starch assays journal January 2009
The unfolded protein response: controlling cell fate decisions under ER stress and beyond journal January 2012
Defects in IRE1 enhance cell death and fail to degrade mRNAs encoding secretory pathway proteins in the Arabidopsis unfolded protein response journal March 2013
Protein kinase and ribonuclease domains of IRE1 confer stress tolerance, vegetative growth, and reproductive development in Arabidopsis journal October 2013
The Lumen-Facing Domain Is Important for the Biological Function and Organelle-to-Organelle Movement of bZIP28 during ER Stress in Arabidopsis journal September 2013
An Endoplasmic Reticulum Stress Response in Arabidopsis Is Mediated by Proteolytic Processing and Nuclear Relocation of a Membrane-Associated Transcription Factor, bZIP28 journal December 2007
Arabidopsis bZIP60 Is a Proteolysis-Activated Transcription Factor Involved in the Endoplasmic Reticulum Stress Response journal November 2008
AtIRE1A/AtIRE1B and AGB1 independently control two essential unfolded protein response pathways in Arabidopsis journal October 2011
Managing the protein folding demands in the endoplasmic reticulum of plants journal March 2016
The Membrane-Associated Transcription Factor NAC089 Controls ER-Stress-Induced Programmed Cell Death in Plants journal March 2014
Conserved and plant-unique strategies for overcoming endoplasmic reticulum stress journal January 2014

Cited By (1)


Similar Records

Functional Diversification of ER Stress Responses in Arabidopsis
Journal Article · Sun Nov 17 19:00:00 EST 2019 · Trends in Biochemical Sciences · OSTI ID:1800727

Systemic signaling contributes to the unfolded protein response of the plant endoplasmic reticulum
Journal Article · Mon Sep 24 20:00:00 EDT 2018 · Nature Communications · OSTI ID:1619789