Effects of tubulin acetylation and tubulin acetyltransferase binding on microtubule structure

Howes, Stuart C.; Alushin, Gregory M.; Shida, Toshinobu; Nachury, Maxence V.; Nogales, Eva

doi:10.1091/mbc.e13-07-0387

Title: Effects of tubulin acetylation and tubulin acetyltransferase binding on microtubule structure

Journal Article · Wed Nov 13 00:00:00 EST 2013 · Molecular Biology of the Cell

DOI:https://doi.org/10.1091/mbc.e13-07-0387· OSTI ID:1625232

Howes, Stuart C. ^[1]; Alushin, Gregory M. ^[2]; Shida, Toshinobu ^[3]; Nachury, Maxence V. ^[3]; Nogales, Eva ^[4]

Univ. of California, Berkeley, CA (United States). Biophysics Graduate Group
Univ. of California, Berkeley, CA (United States).Howard Hughes Medical Inst. Dept. of Molecular and Cell Biology
Stanford Univ., CA (United States). School of Medicine. Dept. of Molecular and Cellular Physiology
Univ. of California, Berkeley, CA (United States).Howard Hughes Medical Inst. Dept. of Molecular and Cell Biology; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Life Sciences Division

Tubulin undergoes posttranslational modifications proposed to specify microtubule subpopulations for particular functions. Most of these modifications occur on the C-termini of tubulin and may directly affect the binding of microtubule-associated proteins (MAPs) or motors. Acetylation of Lys-40 on α-tubulin is unique in that it is located on the luminal surface of microtubules, away from the interaction sites of most MAPs and motors. We investigate whether acetylation alters the architecture of microtubules or the conformation of tubulin, using cryo–electron microscopy (cryo-EM). No significant changes are observed based on protofilament distributions or microtubule helical lattice parameters. Furthermore, no clear differences in tubulin structure are detected between cryo-EM reconstructions of maximally deacetylated or acetylated microtubules. Our results indicate that the effect of acetylation must be highly localized and affect interaction with proteins that bind directly to the lumen of the microtubule. We also investigate the interaction of the tubulin acetyltransferase, αTAT1, with microtubules and find that αTAT1 is able to interact with the outside of the microtubule, at least partly through the tubulin C-termini. Binding to the outside surface of the microtubule could facilitate access of αTAT1 to its luminal site of action if microtubules undergo lateral opening between protofilaments.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1625232

Journal Information:: Molecular Biology of the Cell, Vol. 25, Issue 2; ISSN 1059-1524

Publisher:: American Society for Cell BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Oncogenic microtubule hyperacetylation through BEX4-mediated sirtuin 2 inhibition Lee, Jin-Kwan; Lee, Janet; Go, Heounjeong Cell Death & Disease, Vol. 7, Issue 8 https://doi.org/10.1038/cddis.2016.240	journal	August 2016
αTAT1 downregulation induces mitotic catastrophe in HeLa and A549 cells Chien, J-Y; Tsen, S-D; Chien, C-C Cell Death Discovery, Vol. 2, Issue 1 https://doi.org/10.1038/cddiscovery.2016.6	journal	February 2016
Human histone deacetylase 6 shows strong preference for tubulin dimers over assembled microtubules Skultetyova, Lubica; Ustinova, Kseniya; Kutil, Zsofia Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-11739-3	journal	September 2017
αTAT1 controls longitudinal spreading of acetylation marks from open microtubules extremities Ly, Nathalie; Elkhatib, Nadia; Bresteau, Enzo Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep35624	journal	October 2016
A conceptual view at microtubule plus end dynamics in neuronal axons Voelzmann, Andre; Hahn, Ines; Pearce, Simon P. Brain Research Bulletin https://doi.org/10.1101/062711	journal	August 2016
TgATAT-Mediated α-Tubulin Acetylation Is Required for Division of the Protozoan Parasite Toxoplasma gondii Varberg, Joseph M.; Padgett, Leah R.; Arrizabalaga, Gustavo mSphere, Vol. 1, Issue 1 https://doi.org/10.1128/msphere.00088-15	journal	February 2016
The primary cilium is a self-adaptable, integrating nexus for mechanical stimuli and cellular signaling Nguyen, An M.; Young, Y. -N.; Jacobs, Christopher R. Biology Open, Vol. 4, Issue 12 https://doi.org/10.1242/bio.014787	journal	November 2015
The Altered Hepatic Tubulin Code in Alcoholic Liver Disease Groebner, Jennifer; Tuma, Pamela Biomolecules, Vol. 5, Issue 3 https://doi.org/10.3390/biom5032140	journal	September 2015
High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells Braun, Alexander; Caesar, Nicole M.; Dang, Kyvan Journal of Visualized Experiments, Issue 114 https://doi.org/10.3791/54265	journal	August 2016
Expression of acetylated tubulin in the postnatal developing mouse cochlea Liu, Wenjing; Wang, Chuanxi; Yu, Hao European Journal of Histochemistry https://doi.org/10.4081/ejh.2018.2942	journal	August 2018

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