Effects of α-tubulin acetylation on microtubule structure and stability

Eshun-Wilson, Lisa; Zhang, Rui; Portran, Didier; Nachury, Maxence V.; Toso, Daniel B.; Löhr, Thomas; Vendruscolo, Michele; Bonomi, Massimiliano; Fraser, James S.; Nogales, Eva

doi:10.1073/pnas.1900441116

Title: Effects of α-tubulin acetylation on microtubule structure and stability

Journal Article · Thu May 09 00:00:00 EDT 2019 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1900441116· OSTI ID:1559217

Eshun-Wilson, Lisa ^[1]; Zhang, Rui ^[2]; Portran, Didier ^[3];

^[4]; Toso, Daniel B. ^[1]; Löhr, Thomas ^[5]; Vendruscolo, Michele ^[5]; Bonomi, Massimiliano ^[6]; Fraser, James S. ^[7];

^[8]

Univ. of California, Berkeley, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Washington Univ., St. Louis, MO (United States)
Univ. of California, San Francisco, CA (United States); Univ. Montpellier, Montpellier (France)
Univ. of California, San Francisco, CA (United States)
Univ. of Cambridge, Cambridge (United Kingdom)
Univ. of Cambridge, Cambridge (United Kingdom); Inst. Pasteur, Paris (France)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, San Francisco, CA (United States)
Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Acetylation of K40 in α-tubulin is the sole posttranslational modification to mark the luminal surface of microtubules. It is still controversial whether its relationship with microtubule stabilization is correlative or causative. We have obtained high-resolution cryo-electron microscopy (cryo-EM) reconstructions of pure samples of αTAT1-acetylated and SIRT2-deacetylated microtubules to visualize the structural consequences of this modification and reveal its potential for influencing the larger assembly properties of microtubules. We modeled the conformational ensembles of the unmodified and acetylated states by using the experimental cryo-EM density as a structural restraint in molecular dynamics simulations. We found that acetylation alters the conformational landscape of the flexible loop that contains αK40. Modification of αK40 reduces the disorder of the loop and restricts the states that it samples. We propose that the change in conformational sampling that we describe, at a location very close to the lateral contacts site, is likely to affect microtubule stability and function.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC); National Science Foundation (NSF); National Institute of General Medical Sciences (NIGMS); National Academy of Sciences National Research Council Ford Foundation

Grant/Contract Number:: AC02-05CH11231; 2016222703; R01-GM123159; R35-GM127018; P01-GM063210

OSTI ID:: 1559217

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Vol. 116, Issue 21; ISSN 0027-8424

Publisher:: National Academy of Sciences, Washington, DC (United States)Copyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 136 works

Citation information provided by
Web of Science

References (67)

Microtubules: 50 years on from the discovery of tubulin Borisy, Gary; Heald, Rebecca; Howard, Jonathon Nature Reviews Molecular Cell Biology, Vol. 17, Issue 5 https://doi.org/10.1038/nrm.2016.45	journal	April 2016
PHENIX: a comprehensive Python-based system for macromolecular structure solution. Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.45787	text	January 2010
CHARMM36 all-atom additive protein force field: Validation based on comparison to NMR data Huang, Jing; MacKerell, Alexander D. Journal of Computational Chemistry, Vol. 34, Issue 25 https://doi.org/10.1002/jcc.23354	journal	July 2013
GROMACS 4: Algorithms for Highly Efficient, Load-Balanced, and Scalable Molecular Simulation Hess, Berk; Kutzner, Carsten; van der Spoel, David Journal of Chemical Theory and Computation, Vol. 4, Issue 3 https://doi.org/10.1021/ct700301q	journal	February 2008
Integrative structural and dynamical biology with PLUMED-ISDB Bonomi, Massimiliano; Camilloni, Carlo Bioinformatics, Vol. 33, Issue 24 https://doi.org/10.1093/bioinformatics/btx529	journal	August 2017
The multifaceted role of lysine acetylation in cancer: prognostic biomarker and therapeutic target Di Martile, Marta; Del Bufalo, Donatella; Trisciuoglio, Daniela Oncotarget, Vol. 7, Issue 34 https://doi.org/10.18632/oncotarget.10048	journal	June 2016
Appion: An integrated, database-driven pipeline to facilitate EM image processing Lander, Gabriel C.; Stagg, Scott M.; Voss, Neil R. Journal of Structural Biology, Vol. 166, Issue 1 https://doi.org/10.1016/j.jsb.2009.01.002	journal	April 2009
Determination of protein structural ensembles using cryo-electron microscopy. Bonomi, Massimilano; Vendruscolo, Michele Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.34018	text	January 2019
Mechanistic Origin of Microtubule Dynamic Instability and Its Modulation by EB Proteins Zhang, Rui; Alushin, Gregory M.; Brown, Alan Cell, Vol. 162, Issue 4 https://doi.org/10.1016/j.cell.2015.07.012	journal	August 2015
Posttranslational Acetylation of α-Tubulin Constrains Protofilament Number in Native Microtubules Cueva, Juan G.; Hsin, Jen; Huang, Kerwyn Casey Current Biology, Vol. 22, Issue 12 https://doi.org/10.1016/j.cub.2012.05.012	journal	June 2012
The iterative helical real space reconstruction method: Surmounting the problems posed by real polymers Egelman, Edward H. Journal of Structural Biology, Vol. 157, Issue 1 https://doi.org/10.1016/j.jsb.2006.05.015	journal	January 2007
CTFFIND4: Fast and accurate defocus estimation from electron micrographs Rohou, Alexis; Grigorieff, Nikolaus Journal of Structural Biology, Vol. 192, Issue 2 https://doi.org/10.1016/j.jsb.2015.08.008	journal	November 2015
Simultaneous Determination of Protein Structure and Dynamics Using Cryo-Electron Microscopy Bonomi, Massimiliano; Pellarin, Riccardo; Vendruscolo, Michele Biophysical Journal, Vol. 114, Issue 7 https://doi.org/10.1016/j.bpj.2018.02.028	journal	April 2018
Automated map sharpening by maximization of detail and connectivity Terwilliger, Thomas C.; Sobolev, Oleg V.; Afonine, Pavel V. Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 6 https://doi.org/10.1107/S2059798318004655	journal	May 2018
Conformational changes in tubulin in GMPCPP and GDP-taxol microtubules observed by cryoelectron microscopy Yajima, Hiroaki; Ogura, Toshihiko; Nitta, Ryo The Journal of Cell Biology, Vol. 198, Issue 3 https://doi.org/10.1083/jcb.201201161	journal	July 2012
PLUMED 2: New feathers for an old bird Tribello, Gareth A.; Bonomi, Massimiliano; Branduardi, Davide Computer Physics Communications, Vol. 185, Issue 2 https://doi.org/10.1016/j.cpc.2013.09.018	journal	February 2014
The acetylation of alpha-tubulin and its relationship to the assembly and disassembly of microtubules Maruta, H. The Journal of Cell Biology, Vol. 103, Issue 2 https://doi.org/10.1083/jcb.103.2.571	journal	August 1986
MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy Zheng, Shawn Q.; Palovcak, Eugene; Armache, Jean-Paul Nature Methods, Vol. 14, Issue 4 https://doi.org/10.1038/nmeth.4193	journal	February 2017
Tubulin Posttranslational Modifications and Emerging Links to Human Disease Magiera, Maria M.; Singh, Puja; Gadadhar, Sudarshan Cell, Vol. 173, Issue 6 https://doi.org/10.1016/j.cell.2018.05.018	journal	May 2018
Flexural rigidity of microtubules and actin filaments measured from thermal fluctuations in shape Gittes, F. The Journal of Cell Biology, Vol. 120, Issue 4 https://doi.org/10.1083/jcb.120.4.923	journal	February 1993
Likelihood-based classification of cryo-EM images using FREALIGN Lyumkis, Dmitry; Brilot, Axel F.; Theobald, Douglas L. Journal of Structural Biology, Vol. 183, Issue 3 https://doi.org/10.1016/j.jsb.2013.07.005	journal	September 2013
CHARMM36m: an improved force field for folded and intrinsically disordered proteins Huang, Jing; Rauscher, Sarah; Nawrocki, Grzegorz Nature Methods, Vol. 14, Issue 1 https://doi.org/10.1038/nmeth.4067	journal	November 2016
Determination of protein structural ensembles using cryo-electron microscopy Bonomi, Massimiliano; Vendruscolo, Michele Current Opinion in Structural Biology, Vol. 56 https://doi.org/10.1016/j.sbi.2018.10.006	journal	June 2019
Molecular Basis for Age-Dependent Microtubule Acetylation by Tubulin Acetyltransferase Szyk, Agnieszka; Deaconescu, Alexandra M.; Spector, Jeffrey Cell, Vol. 157, Issue 6 https://doi.org/10.1016/j.cell.2014.03.061	journal	June 2014
HDAC6 inhibitors reverse axonal loss in a mouse model of mutant HSPB1–induced Charcot-Marie-Tooth disease d'Ydewalle, Constantin; Krishnan, Jyothsna; Chiheb, Driss M. Nature Medicine, Vol. 17, Issue 8 https://doi.org/10.1038/nm.2396	journal	July 2011
Structure of the calcium-dependent type 2 secretion pseudopilus López-Castilla, Aracelys; Thomassin, Jenny-Lee; Bardiaux, Benjamin Nature Microbiology, Vol. 2, Issue 12 https://doi.org/10.1038/s41564-017-0041-2	journal	October 2017
Reversible inhibition of the ClpP protease via an N-terminal conformational switch Vahidi, Siavash; Ripstein, Zev A.; Bonomi, Massimiliano Proceedings of the National Academy of Sciences, Vol. 115, Issue 28 https://doi.org/10.1073/pnas.1805125115	journal	June 2018
Evolution and functional cross‐talk of protein post‐translational modifications Beltrao, Pedro; Bork, Peer; Krogan, Nevan J. Molecular Systems Biology, Vol. 9, Issue 1 https://doi.org/10.1002/msb.201304521	journal	January 2013
FREALIGN: High-resolution refinement of single particle structures Grigorieff, Nikolaus Journal of Structural Biology, Vol. 157, Issue 1 https://doi.org/10.1016/j.jsb.2006.05.004	journal	January 2007
Post-translational modifications in the context of therapeutic proteins Walsh, Gary; Jefferis, Roy Nature Biotechnology, Vol. 24, Issue 10 https://doi.org/10.1038/nbt1252	journal	October 2006
PARP1 exhibits enhanced association and catalytic efficiency with γH2A.X-nucleosome Sharma, Deepti; De Falco, Louis; Padavattan, Sivaraman Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-13641-0	journal	December 2019
Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation Tillu, Vikas A.; Rae, James; Gao, Ya Nature Communications, Vol. 12, Issue 1 https://doi.org/10.1038/s41467-021-21035-4	journal	February 2021
Dynamic instability of microtubule growth Mitchison, Tim; Kirschner, Marc Nature, Vol. 312, Issue 5991 https://doi.org/10.1038/312237a0	journal	November 1984
RNA/Peptide Binding Driven by Electrostatics—Insight from Bidirectional Pulling Simulations Do, Trang N.; Carloni, Paolo; Varani, Gabriele Journal of Chemical Theory and Computation, Vol. 9, Issue 3 https://doi.org/10.1021/ct3009914	journal	February 2013
Features and development of Coot Emsley, P.; Lohkamp, B.; Scott, W. G. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4 https://doi.org/10.1107/S0907444910007493	journal	March 2010
Localization of an exchangeable GTP binding site at the plus end of microtubules Mitchison, T. Science, Vol. 261, Issue 5124 https://doi.org/10.1126/science.8102497	journal	August 1993
Physicochemical Properties of Cells and Their Effects on Intrinsically Disordered Proteins (IDPs) Tamara, Frembgen-Kesner,; H., Elcock, Adrian; Mohona, Sarkar, The University of North Carolina at Chapel Hill University Libraries https://doi.org/10.17615/209x-pp25	text	January 2014
Increasing microtubule acetylation rescues axonal transport and locomotor deficits caused by LRRK2 Roc-COR domain mutations Godena, Vinay K.; Brookes-Hocking, Nicholas; Moller, Annekathrin Nature Communications, Vol. 5, Issue 1 https://doi.org/10.1038/ncomms6245	journal	October 2014
High-Resolution Microtubule Structures Reveal the Structural Transitions in αβ-Tubulin upon GTP Hydrolysis Alushin, Gregory M.; Lander, Gabriel C.; Kellogg, Elizabeth H. Cell, Vol. 157, Issue 5 https://doi.org/10.1016/j.cell.2014.03.053	journal	May 2014
Post-translational regulation of the microtubule cytoskeleton: mechanisms and functions Janke, Carsten; Chloë Bulinski, Jeannette Nature Reviews Molecular Cell Biology, Vol. 12, Issue 12 https://doi.org/10.1038/nrm3227	journal	November 2011
Physicochemical Properties of Cells and Their Effects on Intrinsically Disordered Proteins (IDPs) Theillet, Francois-Xavier; Binolfi, Andres; Frembgen-Kesner, Tamara Chemical Reviews, Vol. 114, Issue 13 https://doi.org/10.1021/cr400695p	journal	December 2013
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
Microtubules acquire resistance from mechanical breakage through intralumenal acetylation Xu, Zhenjie; Schaedel, Laura; Portran, Didier Science, Vol. 356, Issue 6335 https://doi.org/10.1126/science.aai8764	journal	April 2017
Causes and Consequences of Microtubule Acetylation Janke, Carsten; Montagnac, Guillaume Current Biology, Vol. 27, Issue 23 https://doi.org/10.1016/j.cub.2017.10.044	journal	December 2017
Identification of an acetylation site of Chlamydomonas alpha-tubulin. LeDizet, M.; Piperno, G. Proceedings of the National Academy of Sciences, Vol. 84, Issue 16 https://doi.org/10.1073/pnas.84.16.5720	journal	August 1987
MDAnalysis: A toolkit for the analysis of molecular dynamics simulations Michaud-Agrawal, Naveen; Denning, Elizabeth J.; Woolf, Thomas B. Journal of Computational Chemistry, Vol. 32, Issue 10 https://doi.org/10.1002/jcc.21787	journal	April 2011
Computational studies of protein regulation by post-translational phosphorylation Narayanan, Arjun; Jacobson, Matthew P. Current Opinion in Structural Biology, Vol. 19, Issue 2 https://doi.org/10.1016/j.sbi.2009.02.007	journal	April 2009
Escherichia coli FtsA forms lipid-bound minirings that antagonize lateral interactions between FtsZ protofilaments Krupka, Marcin; Rowlett, Veronica W.; Morado, Dustin Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/ncomms15957	journal	July 2017
Automated electron microscope tomography using robust prediction of specimen movements Mastronarde, David N. Journal of Structural Biology, Vol. 152, Issue 1 https://doi.org/10.1016/j.jsb.2005.07.007	journal	October 2005
Tubulin acetylation protects long-lived microtubules against mechanical ageing Portran, Didier; Schaedel, Laura; Xu, Zhenjie Nature Cell Biology, Vol. 19, Issue 4 https://doi.org/10.1038/ncb3481	journal	February 2017
A new protocol to accurately determine microtubule lattice seam location Zhang, Rui; Nogales, Eva Journal of Structural Biology, Vol. 192, Issue 2 https://doi.org/10.1016/j.jsb.2015.09.015	journal	November 2015
Cytoplasmic microtubules containing acetylated alpha-tubulin in Chlamydomonas reinhardtii: spatial arrangement and properties LeDizet, M. The Journal of Cell Biology, Vol. 103, Issue 1 https://doi.org/10.1083/jcb.103.1.13	journal	July 1986
HDAC6 Inhibitors Rescued the Defective Axonal Mitochondrial Movement in Motor Neurons Derived from the Induced Pluripotent Stem Cells of Peripheral Neuropathy Patients with HSPB1 Mutation Kim, Ji-Yon; Woo, So-Youn; Hong, Young Bin Stem Cells International, Vol. 2016 https://doi.org/10.1155/2016/9475981	journal	January 2016
EMAN: Semiautomated Software for High-Resolution Single-Particle Reconstructions Ludtke, Steven J.; Baldwin, Philip R.; Chiu, Wah Journal of Structural Biology, Vol. 128, Issue 1 https://doi.org/10.1006/jsbi.1999.4174	journal	December 1999
Effects of tubulin acetylation and tubulin acetyltransferase binding on microtubule structure Howes, Stuart C.; Alushin, Gregory M.; Shida, Toshinobu Molecular Biology of the Cell, Vol. 25, Issue 2 https://doi.org/10.1091/mbc.e13-07-0387	journal	January 2014
A microtubule bestiary: structural diversity in tubulin polymers Chaaban, Sami; Brouhard, Gary J. Molecular Biology of the Cell, Vol. 28, Issue 22 https://doi.org/10.1091/mbc.e16-05-0271	journal	November 2017
RNA/peptide binding driven by electrostatics -- Insight from bi-directional pulling simulations Do, Trang N.; Carloni, Paolo; Varani, Gabriele arXiv https://doi.org/10.48550/arxiv.1307.5565	text	January 2013
Determination of protein structural ensembles using cryo-electron microscopy Bonomi, Massimiliano; Vendruscolo, Michele arXiv https://doi.org/10.48550/arxiv.1810.11306	text	January 2018
High-Resolution Model of the Microtubule Nogales, Eva; Whittaker, Michael; Milligan, Ronald A. Cell, Vol. 96, Issue 1 https://doi.org/10.1016/s0092-8674(00)80961-7	journal	January 1999
Purification of brain tubulin through two cycles of polymerization–depolymerization in a high-molarity buffer Castoldi, Mirco; Popov, Andrei V. Protein Expression and Purification, Vol. 32, Issue 1 https://doi.org/10.1016/s1046-5928(03)00218-3	journal	November 2003
New pathogenetic insights into Charcot–Marie–Tooth disease Malpass, Katy Nature Reviews Neurology, Vol. 7, Issue 9 https://doi.org/10.1038/nrneurol.2011.131	journal	August 2011
Bacterial Filament Systems: Toward Understanding Their Emergent Behavior and Cellular Functions Eun, Ye-Jin; Kapoor, Mrinal; Hussain, Saman Journal of Biological Chemistry, Vol. 290, Issue 28 https://doi.org/10.1074/jbc.r115.637876	journal	May 2015
α-Tubulin Acetylation Elevated in Metastatic and Basal-like Breast Cancer Cells Promotes Microtentacle Formation, Adhesion, and Invasive Migration Boggs, Amanda E.; Vitolo, Michele I.; Whipple, Rebecca A. Cancer Research, Vol. 75, Issue 1 https://doi.org/10.1158/0008-5472.can-13-3563	journal	December 2014
MEC-17 Deficiency Leads to Reduced -Tubulin Acetylation and Impaired Migration of Cortical Neurons Li, L.; Wei, D.; Wang, Q. Journal of Neuroscience, Vol. 32, Issue 37 https://doi.org/10.1523/jneurosci.0016-12.2012	journal	September 2012
Histone Deacetylase 6 Inhibition Compensates for the Transport Deficit in Huntington's Disease by Increasing Tubulin Acetylation Dompierre, J. P.; Godin, J. D.; Charrin, B. C. Journal of Neuroscience, Vol. 27, Issue 13 https://doi.org/10.1523/jneurosci.0037-07.2007	journal	March 2007
PLUMED 2: New feathers for an old bird Tribello, Gareth A.; Bonomi, Massimiliano; Branduardi, Davide arXiv https://doi.org/10.48550/arxiv.1310.0980	text	January 2013
Molecular dynamics-based refinement and validation for sub-5 Å cryo-electron microscopy maps Singharoy, Abhishek; Teo, Ivan; McGreevy, Ryan eLife, Vol. 5 https://doi.org/10.7554/elife.16105	journal	July 2016

Cited By (16)

C-Terminal Tail Polyglycylation and Polyglutamylation Alter Microtubule Mechanical Properties Wall, Kathryn P.; Hart, Harold; Lee, Thomas Biophysical Journal, Vol. 119, Issue 11 https://doi.org/10.1016/j.bpj.2020.09.040	journal	December 2020
The speed of GTP hydrolysis determines GTP cap size and controls microtubule stability. Roostalu, Johanna; Thomas, Claire; Cade, Nicholas Ian The Francis Crick Institute https://doi.org/10.25418/crick.11861826	text	January 2020
Microtubules in Influenza Virus Entry and Egress Simpson, Caitlin; Yamauchi, Yohei Viruses, Vol. 12, Issue 1 https://doi.org/10.3390/v12010117	journal	January 2020
Homogeneous multifocal excitation for high-throughput super-resolution imaging Mahecic, Dora; Gambarotto, Davide; Douglass, Kyle M. Nature Methods, Vol. 17, Issue 7 https://doi.org/10.1038/s41592-020-0859-z	journal	June 2020
Cytoskeletal makeup of the synapse: Shaft versus spine Bucher, Michael; Fanutza, Tomas; Mikhaylova, Marina Cytoskeleton https://doi.org/10.1002/cm.21583	journal	December 2019
The inner junction complex of the cilia is an interaction hub that involves tubulin post-translational modifications Khalifa, Ahmad Abdelzaher Zaki; Ichikawa, Muneyoshi; Dai, Daniel eLife, Vol. 9 https://doi.org/10.7554/elife.52760	journal	January 2020
Tubulin Acetylation Mediates Bisphenol A Effects on the Microtubule Arrays of Allium cepa and Triticum turgidum Adamakis, Ioannis-Dimosthenis S.; Panteris, Emmanuel; Eleftheriou, Eleftherios P. Biomolecules, Vol. 9, Issue 5 https://doi.org/10.3390/biom9050185	journal	May 2019
The Inner Junction Complex of the Cilia is an Interaction Hub that Involves Tubulin Post-translational Modifications Khalifa, Ahmad; Ichikawa, Muneyoshi; Dai, Daniel Biophysical Journal, Vol. 118, Issue 3 https://doi.org/10.1016/j.bpj.2019.11.349	journal	February 2020
Trainable Weka Segmentation: a machine learning tool for microscopy pixel classification Arganda-Carreras, Ignacio; Kaynig, Verena; Rueden, Curtis Bioinformatics, Vol. 33, Issue 15 https://doi.org/10.1093/bioinformatics/btx180	journal	March 2017
Suppressing migration and invasion of H1299 lung cancer cells by honokiol through disrupting expression of an HDAC6‐mediated matrix metalloproteinase 9 Pai, Jih‐Tung; Hsu, Chia‐Yun; Hsieh, Yei‐San Food Science & Nutrition, Vol. 8, Issue 3 https://doi.org/10.1002/fsn3.1439	journal	February 2020
Direct observation of dynamic protein interactions involving human microtubules using solid-state NMR spectroscopy Luo, Yanzhang; Xiang, ShengQi; Hooikaas, Peter Jan Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-019-13876-x	journal	January 2020
The speed of GTP hydrolysis determines GTP cap size and controls microtubule stability. Roostalu, Johanna; Thomas, Claire; Cade, Nicholas Ian The Francis Crick Institute https://doi.org/10.25418/crick.11861826.v1	text	January 2020
What Will Computational Modeling Approaches Have to Say in the Era of Atomistic Cryo-EM Data? Fraser, James S.; Lindorff-Larsen, Kresten; Bonomi, Massimiliano Journal of Chemical Information and Modeling, Vol. 60, Issue 5 https://doi.org/10.1021/acs.jcim.0c00123	journal	February 2020
Lysine Acetylation, Cancer Hallmarks and Emerging Onco-Therapeutic Opportunities Hu, Meilan; He, Fule; Thompson, Erik W. Cancers, Vol. 14, Issue 2 https://doi.org/10.3390/cancers14020346	journal	January 2022
PAK1 Regulates MEC-17 Acetyltransferase Activity and Microtubule Acetylation during Proplatelet Extension van Dijk, Juliette; Bompard, Guillaume; Rabeharivelo, Gabriel International Journal of Molecular Sciences, Vol. 21, Issue 20 https://doi.org/10.3390/ijms21207531	journal	October 2020
Molecular Evolution of Tubulins in Diatoms Khabudaev, Kirill V.; Petrova, Darya P.; Bedoshvili, Yekaterina D. International Journal of Molecular Sciences, Vol. 23, Issue 2 https://doi.org/10.3390/ijms23020618	journal	January 2022

Similar Records

Effects of tubulin acetylation and tubulin acetyltransferase binding on microtubule structure

Journal Article · Wed Nov 13 00:00:00 EST 2013 · Molecular Biology of the Cell · OSTI ID:1559217

Howes, Stuart C.; Alushin, Gregory M.; Shida, Toshinobu; +2 more

Genetic disruption of tubulin acetyltransferase, αTAT1, inhibits proliferation and invasion of colon cancer cells through decreases in Wnt1/β-catenin signaling

Journal Article · Sun Jan 01 00:00:00 EST 2017 · Biochemical and Biophysical Research Communications · OSTI ID:1559217

Oh, Somi; You, Eunae; Ko, Panseon; +3 more

The Dam1 ring binds to the E-hook of tubulin and diffuses along the microtubule

Journal Article · Fri Dec 17 00:00:00 EST 2010 · Molecular Biology of the Cell · OSTI ID:1559217

Ramey, Vincent H.; Wang, Hong-Wei; Nakajima, Yuko; +5 more

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
cryo-EM
MD
tubulin modifications
microtubule
acetylation

Title: Effects of α-tubulin acetylation on microtubule structure and stability

Citation Formats

References (67)

Cited By (16)

Similar Records

Related Subjects