PLP inhibits the activity of interphase centrosomes to ensure their proper segregation in stem cells

Lerit, Dorothy A.; Rusan, Nasser M.

doi:10.1083/jcb.201303141

PLP inhibits the activity of interphase centrosomes to ensure their proper segregation in stem cells

Journal Article · Mon Sep 30 00:00:00 EDT 2013 · Journal of Cell Biology

DOI:https://doi.org/10.1083/jcb.201303141· OSTI ID:1625167

Lerit, Dorothy A. ^[1]; Rusan, Nasser M. ^[2]

National Institutes of Health (NIH), Bethesda, MD (United States). National Heart, Lung, and Blood Inst. Cell Biology and Physiology Center; DOE/OSTI
National Institutes of Health (NIH), Bethesda, MD (United States). National Heart, Lung, and Blood Inst. Cell Biology and Physiology Center

Centrosomes determine the mitotic axis of asymmetrically dividing stem cells. Several studies have shown that the centrosomes of the Drosophila melanogaster central brain neural stem cells are themselves asymmetric, organizing varying levels of pericentriolar material and microtubules. This asymmetry produces one active and one inactive centrosome during interphase. We identify pericentrin-like protein (PLP) as a negative regulator of centrosome maturation and activity. We show that PLP is enriched on the inactive interphase centrosome, where it blocks recruitment of the master regulator of centrosome maturation, Polo kinase. Furthermore, we find that ectopic Centrobin expression influenced PLP levels on the basal centrosome, suggesting it may normally function to regulate PLP. Finally, we conclude that, although asymmetric centrosome maturation is not required for asymmetric cell division, it is required for proper centrosome segregation to the two daughter cells.

View Accepted Manuscript (DOE)

Research Organization:: National Institutes of Health (NIH), Bethesda, MD (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

OSTI ID:: 1625167

Journal Information:: Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 7 Vol. 202; ISSN 0021-9525

Publisher:: Rockefeller University PressCopyright Statement

Country of Publication:: United States

Language:: English

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