Epimorphin Mediates Mammary Luminal Morphogenesis through Control of C/EBPβ

Hirai, Yohei; Radisky, Derek; Boudreau, Rosanne; Simian, Marina; Stevens, Mary E.; Oka, Yumiko; Takebe, Kyoko; Niwa, Shinichiro; Bissell, Mina J.

doi:10.1083/jcb.153.4.785

Epimorphin Mediates Mammary Luminal Morphogenesis through Control of C/EBPβ

Journal Article · Mon May 14 00:00:00 EDT 2001 · Journal of Cell Biology

DOI:https://doi.org/10.1083/jcb.153.4.785· OSTI ID:1625124

Hirai, Yohei ^[1]; Radisky, Derek ^[2]; Boudreau, Rosanne ^[2]; Simian, Marina ^[2]; Stevens, Mary E. ^[3]; Oka, Yumiko ^[4]; Takebe, Kyoko ^[4]; Niwa, Shinichiro ^[4]; Bissell, Mina J. ^[2]

Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Life Science Division; Sumitomo Electric Industries Ltd., Yokohama (Japan). Osaka R&D Lab. (Yokohama-lab); DOE/OSTI
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Life Science Division
Life Science Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94720
Sumitomo Electric Industries Ltd., Yokohama (Japan). Osaka R&D Lab. (Yokohama-lab)

We have shown previously that epimorphin (EPM), a protein expressed on the surface of myoepithelial and fibroblast cells of the mammary gland, acts as a multifunctional morphogen of mammary epithelial cells. Here, we present the molecular mechanism by which EPM mediates luminal morphogenesis. Treatment of cells with EPM to induce lumen formation greatly increases the overall expression of transcription factor CCAAT/enhancer binding protein (C/EBP)β and alters the relative expression of its two principal isoforms, LIP and LAP. These alterations were shown to be essential for the morphogenetic activities, since constitutive expression of LIP was sufficient to produce lumen formation, whereas constitutive expression of LAP blocked EPM-mediated luminal morphogenesis. Furthermore, in a transgenic mouse model in which EPM expression was expressed in an apolar fashion on the surface of mammary epithelial cells, we found increased expression of C/EBPβ, increased relative expression of LIP to LAP, and enlarged ductal lumina. Together, our studies demonstrate a role for EPM in luminal morphogenesis through control of C/EBPβ expression.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1625124

Journal Information:: Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 4 Vol. 153; ISSN 0021-9525

Publisher:: Rockefeller University PressCopyright Statement

Country of Publication:: United States

Language:: English

References (87)

LAP (NF-IL-6), a tissue-specific transcriptional activator, is an inhibitor of hepatoma cell proliferation. Buck, M.; Turler, H.; Chojkier, M. The EMBO Journal, Vol. 13, Issue 4 https://doi.org/10.1002/j.1460-2075.1994.tb06328.x	journal	February 1994
A dramatic shift in the transmembrane topology of a viral envelope glycoprotein accompanies hepatitis B viral morphogenesis. Ostapchuk, P.; Hearing, P.; Ganem, D. The EMBO Journal, Vol. 13, Issue 5 https://doi.org/10.1002/j.1460-2075.1994.tb06353.x	journal	March 1994
Transmembrane orientation of signal-anchor proteins is affected by the folding state but not the size of the N-terminal domain. Denzer, A. J.; Nabholz, C. E.; Spiess, M. The EMBO Journal, Vol. 14, Issue 24 https://doi.org/10.1002/j.1460-2075.1995.tb00321.x	journal	December 1995
Lymphoproliferative disorder and imbalanced T-helper response in C/EBP beta-deficient mice. Screpanti, I.; Romani, L.; Musiani, P. The EMBO Journal, Vol. 14, Issue 9 https://doi.org/10.1002/j.1460-2075.1995.tb07185.x	journal	May 1995
Molecular Cloning of Human Epimorphin: Identification of Isoforms and Their Unique Properties Hirai, Y. Biochemical and Biophysical Research Communications, Vol. 191, Issue 3 https://doi.org/10.1006/bbrc.1993.1363	journal	March 1993
A New Function of Ito Cells in Liver Morphogenesis: Evidence Using a Novel Morphogenic Protein, Epimorphin,in Vitro Hirose, Miyoko; Watanabe, Sumio; Oide, Hirosumi Biochemical and Biophysical Research Communications, Vol. 225, Issue 1 https://doi.org/10.1006/bbrc.1996.1146	journal	August 1996
Identification of Cellular Recognition Sequence of Epimorphin and Critical Role of Cell/Epimorphin Interaction in Lung Branching Morphogenesis Koshida, Shogo; Hirai, Yohei Biochemical and Biophysical Research Communications, Vol. 234, Issue 2 https://doi.org/10.1006/bbrc.1997.6673	journal	May 1997
A Novel Hepatic Stellate (Ito) Cell-Derived Protein, Epimorphin, Plays a Key Role in the Late Stages of Liver Regeneration Watanabe, Sumio; Hirose, Miyoko; Wang, Xian-En Biochemical and Biophysical Research Communications, Vol. 250, Issue 2 https://doi.org/10.1006/bbrc.1998.9339	journal	September 1998
Selective induction of CCAAT/enhancer binding protein isoforms occurs during rat liver development Diehl, Anna Mae; Michaelson, Philip; Yang, Shi Qi Gastroenterology, Vol. 106, Issue 6 https://doi.org/10.1016/0016-5085(94)90420-0	journal	June 1994
A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the sam mRNA Descombes, Patrick; Schibler, Ueli Cell, Vol. 67, Issue 3 https://doi.org/10.1016/0092-8674(91)90531-3	journal	November 1991
Epithelial morphogenesis Gumbiner, Barry M. Cell, Vol. 69, Issue 3 https://doi.org/10.1016/0092-8674(92)90440-n	journal	May 1992
Is epimorphin involved in vesicular transport? Pelham, Hugh R. B. Cell, Vol. 73, Issue 3 https://doi.org/10.1016/0092-8674(93)90128-d	journal	May 1993
Membrane fusion takes excitatory turn: Syntaxin, vesicle docking protein, or glutamate receptor? Brose, Nils Cell, Vol. 75, Issue 6 https://doi.org/10.1016/0092-8674(93)90313-f	journal	December 1993
The syntaxin family of vesicular transport receptors Bennett, Mark K.; Garcia-Arrarás, JoséE.; Elferink, Lisa A. Cell, Vol. 74, Issue 5 https://doi.org/10.1016/0092-8674(93)90466-4	journal	September 1993
Targeted disruption of the NF-IL6 gene discloses its essential role in bacteria killing and tumor cytotoxicity by macrophages Tanaka, Takashi; Akira, Shizuo; Yoshida, Kanji Cell, Vol. 80, Issue 2 https://doi.org/10.1016/0092-8674(95)90418-2	journal	January 1995
ECM and Cell Surface Proteolysis: Regulating Cellular Ecology Werb, Zena Cell, Vol. 91, Issue 4 https://doi.org/10.1016/s0092-8674(00)80429-8	journal	November 1997
Metalloprotease-Disintegrins: Links to Cell Adhesion and Cleavage of TNFα and Notch Blobel, Carl P. Cell, Vol. 90, Issue 4 https://doi.org/10.1016/s0092-8674(00)80519-x	journal	August 1997
Relocation of the t-SNARE SNAP-23 from Lamellipodia-like Cell Surface Projections Regulates Compound Exocytosis in Mast Cells Guo, Zhenheng; Turner, Christopher; Castle, David Cell, Vol. 94, Issue 4 https://doi.org/10.1016/s0092-8674(00)81594-9	journal	August 1998
Immunolocalization of epimorphin in skin Butt, Khalid Iqbal; Manabe, Motomu; Yaguchi, Hitoshi Journal of Dermatological Science, Vol. 13, Issue 3 https://doi.org/10.1016/s0923-1811(96)00534-8	journal	December 1996
Molecular mechanisms of liver development and differentiation Darlington, G. Current Opinion in Cell Biology, Vol. 11, Issue 6 https://doi.org/10.1016/s0955-0674(99)00035-6	journal	December 1999
Novel isoform of syntaxin 1 is expressed in mammalian cells Jagadish, Mittur N.; Tellam, Judy T.; Macaulay, S. Lance Biochemical Journal, Vol. 321, Issue 1 https://doi.org/10.1042/bj3210151	journal	January 1997
Induction of Long-Term Potentiation In Vivo Regulates Alternate Splicing to Alter Syntaxin 3 Isoform Expression in Rat Dentate Gyrus Rodger, Jennifer; Davis, Sabrina; Laroche, Serge Journal of Neurochemistry, Vol. 71, Issue 2 https://doi.org/10.1046/j.1471-4159.1998.71020666.x	journal	November 2002
CCAAT/enhancer-binding protein mRNA is translated into multiple proteins with different transcription activation potentials. Ossipow, V.; Descombes, P.; Schibler, U. Proceedings of the National Academy of Sciences, Vol. 90, Issue 17 https://doi.org/10.1073/pnas.90.17.8219	journal	September 1993
Inhibition of CCAAT/Enhancer-binding Protein α and β Translation by Upstream Open Reading Frames Lincoln, A. Jeannine; Monczak, Yury; Williams, Simon C. Journal of Biological Chemistry, Vol. 273, Issue 16 https://doi.org/10.1074/jbc.273.16.9552	journal	April 1998
Biological Role of the CCAAT/Enhancer-binding Protein Family of Transcription Factors Lekstrom-Himes, Julie; Xanthopoulos, Kleanthis G. Journal of Biological Chemistry, Vol. 273, Issue 44 https://doi.org/10.1074/jbc.273.44.28545	journal	November 1998
Generation of Truncated C/EBPβ Isoforms by in VitroProteolysis Baer, Mark; Johnson, Peter F. Journal of Biological Chemistry, Vol. 275, Issue 34 https://doi.org/10.1074/jbc.m004268200	journal	August 2000
Multiple Determinants Direct the Orientation of Signal–Anchor Proteins: The Topogenic Role of the Hydrophobic Signal Domain Wahlberg, Johanna M.; Spiess, Martin Journal of Cell Biology, Vol. 137, Issue 3 https://doi.org/10.1083/jcb.137.3.555	journal	May 1997
Effects of Age on the Posttranscriptional Regulation of CCAAT/Enhancer Binding Protein α and CCAAT/Enhancer Binding Protein β Isoform Synthesis in Control and LPS-Treated Livers Hsieh, Ching-Chyuan; Xiong, Wei; Xie, Qizhi Molecular Biology of the Cell, Vol. 9, Issue 6 https://doi.org/10.1091/mbc.9.6.1479	journal	June 1998
CUG repeat binding protein (CUGBP1) interacts with the 5' region of C/EBP mRNA and regulates translation of C/EBP isoforms Timchenko, N. A.; Lu, A. L.; Welm, X. Nucleic Acids Research, Vol. 27, Issue 22 https://doi.org/10.1093/nar/27.22.4517	journal	November 1999
An essential role for C/EBPbeta in female reproduction Sterneck, E.; Tessarollo, L.; Johnson, P. F. Genes & Development, Vol. 11, Issue 17 https://doi.org/10.1101/gad.11.17.2153	journal	September 1997
The C/EBPbeta transcription factor regulates epithelial cell proliferation and differentiation in the mammary gland Robinson, G. W.; Johnson, P. F.; Hennighausen, L. Genes & Development, Vol. 12, Issue 12 https://doi.org/10.1101/gad.12.12.1907	journal	June 1998
C/EBPbeta , but not C/EBPalpha , is essential for ductal morphogenesis, lobuloalveolar proliferation, and functional differentiation in the mouse mammary gland Seagroves, T. N.; Krnacik, S.; Raught, B. Genes & Development, Vol. 12, Issue 12 https://doi.org/10.1101/gad.12.12.1917	journal	June 1998
Think globally, act locally: the making of a mouse mammary gland Hennighausen, L.; Robinson, G. W. Genes & Development, Vol. 12, Issue 4 https://doi.org/10.1101/gad.12.4.449	journal	February 1998
Sodium-Dodecyl-Sulfate-Resistant Complex Formation of Epimorphin Monomers and Interaction of the 150-kDa Complex with the Cell Surface Hirai, Yohei European Journal of Biochemistry, Vol. 225, Issue 3 https://doi.org/10.1111/j.1432-1033.1994.1133b.x	journal	November 1994
Characterization of a presynaptic glutamate receptor Smirnova, T.; Stinnakre, J.; Mallet, J. Science, Vol. 262, Issue 5132 https://doi.org/10.1126/science.8105537	journal	October 1993
Transsynaptic expression of a presynaptic glutamate receptor during hippocampal long-term potentiation Smirnova, T.; Laroche, S.; Errington, M. Science, Vol. 262, Issue 5132 https://doi.org/10.1126/science.8105538	journal	October 1993
Evidence for posttranscriptional regulation of C/EBPalpha and C/EBPbeta isoform expression during the lipopolysaccharide-mediated acute-phase response. An, M. R.; Hsieh, C. C.; Reisner, P. D. Molecular and Cellular Biology, Vol. 16, Issue 5 https://doi.org/10.1128/mcb.16.5.2295	journal	May 1996
The ability of C/EBP beta but not C/EBP alpha to synergize with an Sp1 protein is specified by the leucine zipper and activation domain. Lee, Y. H.; Williams, S. C.; Baer, M. Molecular and Cellular Biology, Vol. 17, Issue 4 https://doi.org/10.1128/mcb.17.4.2038	journal	April 1997
C/EBPβ Modulates the Early Events of Keratinocyte Differentiation Involving Growth Arrest and Keratin 1 and Keratin 10 Expression Zhu, Songyun; Oh, Hye-Sun; Shim, Minsub Molecular and Cellular Biology, Vol. 19, Issue 10 https://doi.org/10.1128/mcb.19.10.7181	journal	October 1999
SRα Promoter: an Efficient and Versatile Mammalian cDNA Expression System Composed of the Simian Virus 40 Early Promoter and the R-U5 Segment of Human T-Cell Leukemia Virus Type 1 Long Terminal Repeat Takebe, Yutaka; Seiki, Motoharu; Fujisawa, Jun-Ichi Molecular and Cellular Biology, Vol. 8, Issue 1 https://doi.org/10.1128/mcb.8.1.466-472.1988	journal	January 1988
Time course of milk accumulation-induced opening of mammary tight junctions, and blood clearance of milk components Stelwagen, K.; Farr, V. C.; McFadden, H. A. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, Vol. 273, Issue 1 https://doi.org/10.1152/ajpregu.1997.273.1.r379	journal	July 1997
Hormones and Growth Factors in Milk Grosvenor, Clark E.; Picciano, Mary Frances; Baumrucker, Craig R. Endocrine Reviews, Vol. 14, Issue 6 https://doi.org/10.1210/edrv-14-6-710	journal	December 1993
Characterization of SNARE protein expression in beta cell lines and pancreatic islets. Wheeler, M. B.; Sheu, L.; Ghai, M. Endocrinology, Vol. 137, Issue 4 https://doi.org/10.1210/endo.137.4.8625909	journal	April 1996
Comparison of the Regulation of the Whey Acidic Protein Gene with that of a Hybrid Gene Containing the Whey Acidic Protein Gene Promoter in Transgenic Mice Pittius, Christoph W.; Sankaran, Lakshmanan; Topper, Yale J. Molecular Endocrinology, Vol. 2, Issue 11 https://doi.org/10.1210/mend-2-11-1027	journal	November 1988
C/EBPβ (CCAAT/Enhancer Binding Protein) Controls Cell Fate Determination during Mammary Gland Development Seagroves, Tiffany N.; Lydon, John P.; Hovey, Russell C. Molecular Endocrinology, Vol. 14, Issue 3 https://doi.org/10.1210/mend.14.3.0434	journal	March 2000
Developmentally and hormonally regulated CCAAT/enhancer-binding protein isoforms influence beta-casein gene expression. Raught, B.; Liao, W. S.; Rosen, J. M. Molecular Endocrinology, Vol. 9, Issue 9 https://doi.org/10.1210/mend.9.9.7491114	journal	September 1995
Apoptosis in the terminal endbud of the murine mammary gland: a mechanism of ductal morphogenesis Humphreys, R. C.; Krajewska, M.; Krnacik, S. Development, Vol. 122, Issue 12 https://doi.org/10.1242/dev.122.12.4013	journal	December 1996
Signaling through the stromal epidermal growth factor receptor is necessary for mammary ductal development Wiesen, J. F.; Young, P.; Werb, Z. Development, Vol. 126, Issue 2 https://doi.org/10.1242/dev.126.2.335	journal	January 1999
Syntaxin 2 splice variants exhibit differential expression patterns, biochemical properties and subcellular localizations Quinones, B.; Riento, K.; Olkkonen, V. M. Journal of Cell Science, Vol. 112, Issue 23 https://doi.org/10.1242/jcs.112.23.4291	journal	December 1999
Non-functional role of syntaxin 2 in insulin exocytosis by pancreatic β cells Nagamatsu, Shinya; Sawa, Hiroki; Nakamichi, Yoko Cell Biochemistry and Function, Vol. 15, Issue 4 https://doi.org/10.1002/(SICI)1099-0844(199712)15:4<237::AID-CBF746>3.0.CO;2-U	journal	December 1997
Inductive Influences of Epimorphin on Endothelial Cellsin Vitro Oka, Yumiko; Hirai, Yohei Experimental Cell Research, Vol. 222, Issue 1 https://doi.org/10.1006/excr.1996.0024	journal	January 1996
Heads or tails - what determines the orientation of proteins in the membrane Spiess, Martin FEBS Letters, Vol. 369, Issue 1 https://doi.org/10.1016/0014-5793(95)00551-J	journal	August 1995
Epimorphin: A mesenchymal protein essential for epithelial morphogenesis Hirai, Yohei; Takebe, Kyoko; Takashina, Makoto Cell, Vol. 69, Issue 3 https://doi.org/10.1016/0092-8674(92)90448-L	journal	May 1992
Neu differentiation factor: A transmembrane glycoprotein containing an EGF domain and an immunoglobulin homology unit Wen, Duanzhi; Peles, Elior; Cupples, Rod Cell, Vol. 69, Issue 3 https://doi.org/10.1016/0092-8674(92)90456-M	journal	May 1992
Is epimorphin involved in vesicular transport? Pelham, Hugh R. B. Cell, Vol. 73, Issue 3 https://doi.org/10.1016/0092-8674(93)90128-D	journal	May 1993
Reexamination of the properties of epimorphin and its possible roles Hirai, Yohei; Nakagawa, Shin-ichi; Takeichi, Masatoshi Cell, Vol. 73, Issue 3 https://doi.org/10.1016/0092-8674(93)90129-E	journal	May 1993
Membrane fusion takes excitatory turn: Syntaxin, vesicle docking protein, or glutamate receptor? Brose, Nils Cell, Vol. 75, Issue 6 https://doi.org/10.1016/0092-8674(93)90313-F	journal	December 1993
Evidence for an alternate model of human P-glycoprotein structure and biogenesis. Skach, W. R.; Calayag, M. C.; Lingappa, V. R. Journal of Biological Chemistry, Vol. 268, Issue 10 https://doi.org/10.1016/S0021-9258(18)53125-2	journal	April 1993
Translational Induction of Liver-enriched Transcriptional Inhibitory Protein during Acute Phase Response Leads to Repression of CCAAT/Enhancer Binding Protein α mRNA Welm, Alana L.; Mackey, Stephanie L.; Timchenko, Lubov T. Journal of Biological Chemistry, Vol. 275, Issue 35 https://doi.org/10.1016/S0021-9258(19)61525-5	journal	September 2000
ECM and Cell Surface Proteolysis: Regulating Cellular Ecology Werb, Zena Cell, Vol. 91, Issue 4 https://doi.org/10.1016/S0092-8674(00)80429-8	journal	November 1997
Metalloprotease-Disintegrins: Links to Cell Adhesion and Cleavage of TNFα and Notch Blobel, Carl P. Cell, Vol. 90, Issue 4 https://doi.org/10.1016/S0092-8674(00)80519-X	journal	August 1997
Relocation of the t-SNARE SNAP-23 from Lamellipodia-like Cell Surface Projections Regulates Compound Exocytosis in Mast Cells Guo, Zhenheng; Turner, Christopher; Castle, David Cell, Vol. 94, Issue 4 https://doi.org/10.1016/S0092-8674(00)81594-9	journal	August 1998
Immunolocalization of epimorphin in skin Butt, Khalid Iqbal; Manabe, Motomu; Yaguchi, Hitoshi Journal of Dermatological Science, Vol. 13, Issue 3 https://doi.org/10.1016/S0923-1811(96)00534-8	journal	December 1996
Molecular mechanisms of liver development and differentiation Darlington, G. Current Opinion in Cell Biology, Vol. 11, Issue 6 https://doi.org/10.1016/S0955-0674(99)00035-6	journal	December 1999
Origin and Secretion of Milk Lipids Mather, Ian H.; Keenan, Thomas W. Journal of Mammary Gland Biology and Neoplasia, Vol. 3, Issue 3, p. 259-273 https://doi.org/10.1023/A:1018711410270	journal	January 1998
Secretion of Milk Proteins Burgoyne, Robert D.; Duncan, Jennifer S. Journal of Mammary Gland Biology and Neoplasia, Vol. 3, Issue 3, p. 275-286 https://doi.org/10.1023/A:1018763427108	journal	January 1998
Transferrin and Prolactin Transcytosis in the Lactating Mammary Epithelial Cell Ollivier-Bousquet, Michele Journal of Mammary Gland Biology and Neoplasia, Vol. 3, Issue 3, p. 303-313 https://doi.org/10.1023/A:1018767528017	journal	July 1998
Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 Å resolution Sutton, R. Bryan; Fasshauer, Dirk; Jahn, Reinhard Nature, Vol. 395, Issue 6700 https://doi.org/10.1038/26412	journal	September 1998
Mechanisms of intracellular protein transport Rothman, James E. Nature, Vol. 372, Issue 6501 https://doi.org/10.1038/372055a0	journal	November 1994
Extracellular matrix-dependent tissue-specific gene expression in mammary epithelial cells requires both physical and biochemical signal transduction. Roskelley, C. D.; Desprez, P. Y.; Bissell, M. J. Proceedings of the National Academy of Sciences, Vol. 91, Issue 26 https://doi.org/10.1073/pnas.91.26.12378	journal	December 1994
A conserved domain is present in different families of vesicular fusion proteins: A new superfamily Weimbs, T.; Low, S. H.; Chapin, S. J. Proceedings of the National Academy of Sciences, Vol. 94, Issue 7 https://doi.org/10.1073/pnas.94.7.3046	journal	April 1997
Generation of Truncated C/EBPβ Isoforms by in VitroProteolysis Baer, Mark; Johnson, Peter F. Journal of Biological Chemistry, Vol. 275, Issue 34 https://doi.org/10.1074/jbc.M004268200	journal	August 2000
Targeted expression of stromelysin-1 in mammary gland provides evidence for a role of proteinases in branching morphogenesis and the requirement for an intact basement membrane for tissue-specific gene expression [published erratum appears in J Cell Biol 1996 Feb;132(4):following 752] Sympson, C. J. The Journal of Cell Biology, Vol. 125, Issue 3 https://doi.org/10.1083/jcb.125.3.681	journal	May 1994
Epimorphin Functions as a Key Morphoregulator for Mammary Epithelial Cells Hirai, Yohei; Lochter, André; Galosy, Sybille Journal of Cell Biology, Vol. 140, Issue 1 https://doi.org/10.1083/jcb.140.1.159	journal	January 1998
Autocrine Stimulation of Human Pancreatic Duct–Like Development by Soluble Isoforms of Epimorphin in Vitro Lehnert, Lasse; Lerch, Markus M.; Hirai, Yohei Journal of Cell Biology, Vol. 152, Issue 5 https://doi.org/10.1083/jcb.152.5.911	journal	February 2001
A novel transcriptional enhancer is involved in the prolactin- and extracellular matrix-dependent regulation of beta-casein gene expression. Schmidhauser, C.; Casperson, G. F.; Myers, C. A. Molecular Biology of the Cell, Vol. 3, Issue 6 https://doi.org/10.1091/mbc.3.6.699	journal	June 1992
An isoform of the Golgi t-SNARE, syntaxin 5, with an endoplasmic reticulum retrieval signal. Hui, N.; Nakamura, N.; Sönnichsen, B. Molecular Biology of the Cell, Vol. 8, Issue 9 https://doi.org/10.1091/mbc.8.9.1777	journal	September 1997
Defective adipocyte differentiation in mice lacking the C/EBPbeta and/or C/EBPdelta gene Tanaka, T. The EMBO Journal, Vol. 16, Issue 24 https://doi.org/10.1093/emboj/16.24.7432	journal	December 1997
Regenerating livers of old rats contain high levels of C/EBPalpha that correlate with altered expression of cell cycle associated proteins Timchenko, N. Nucleic Acids Research, Vol. 26, Issue 13 https://doi.org/10.1093/nar/26.13.3293	journal	July 1998
Translational control of C/EBPα and C/EBPβ isoform expression Calkhoven, Cor F.; Müller, Christine; Leutz, Achim Genes & Development, Vol. 14, Issue 15 https://doi.org/10.1101/gad.14.15.1920	journal	August 2000
Evidence for posttranscriptional regulation of C/EBPalpha and C/EBPbeta isoform expression during the lipopolysaccharide-mediated acute-phase response. An, M. R.; Hsieh, C. C.; Reisner, P. D. Molecular and Cellular Biology, Vol. 16, Issue 5 https://doi.org/10.1128/MCB.16.5.2295	journal	May 1996
The ability of C/EBP beta but not C/EBP alpha to synergize with an Sp1 protein is specified by the leucine zipper and activation domain. Lee, Y. H.; Williams, S. C.; Baer, M. Molecular and Cellular Biology, Vol. 17, Issue 4 https://doi.org/10.1128/MCB.17.4.2038	journal	April 1997
C/EBPβ Modulates the Early Events of Keratinocyte Differentiation Involving Growth Arrest and Keratin 1 and Keratin 10 Expression Zhu, Songyun; Oh, Hye-Sun; Shim, Minsub Molecular and Cellular Biology, Vol. 19, Issue 10 https://doi.org/10.1128/MCB.19.10.7181	journal	October 1999
C/EBPα Regulates Generation of C/EBPβ Isoforms through Activation of Specific Proteolytic Cleavage Welm, Alana L.; Timchenko, Nikolai A.; Darlington, Gretchen J. Molecular and Cellular Biology, Vol. 19, Issue 3 https://doi.org/10.1128/MCB.19.3.1695	journal	March 1999
SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. Takebe, Y.; Seiki, M.; Fujisawa, J. Molecular and Cellular Biology, Vol. 8, Issue 1 https://doi.org/10.1128/MCB.8.1.466	journal	January 1988
Membrane Fusion and Exocytosis Jahn, Reinhard; Südhof, Thomas C. Annual Review of Biochemistry, Vol. 68, Issue 1 https://doi.org/10.1146/annurev.biochem.68.1.863	journal	June 1999
Autocrine Signals Control CCAAT/Enhancer Binding Protein β Expression, Localization, and Activity in Macrophages Baer, Mark; Williams, Simon C.; Dillner, Allan Blood, Vol. 92, Issue 11 https://doi.org/10.1182/blood.V92.11.4353	journal	December 1998

Cited By (15)

Matrix Metalloproteinase-Induced Epithelial-Mesenchymal Transition in Breast Cancer Radisky, Evette S.; Radisky, Derek C. Journal of Mammary Gland Biology and Neoplasia, Vol. 15, Issue 2 https://doi.org/10.1007/s10911-010-9177-x	journal	May 2010
Angiotensin stimulation of adrenal fasciculata cells Finn, Frances M.; Stehle, Christine; Ricci, Paola Archives of Biochemistry and Biophysics, Vol. 264, Issue 1 https://doi.org/10.1016/0003-9861(88)90581-4	journal	July 1988
Epimorphin acts extracellularly to promote cell sorting and aggregation during the condensation of vertebral cartilage Oka, Yumiko; Sato, Yuki; Tsuda, Hokari Developmental Biology, Vol. 291, Issue 1 https://doi.org/10.1016/j.ydbio.2005.12.001	journal	March 2006
Membrane translocation of t-SNARE protein syntaxin-4 abrogates ground-state pluripotency in mouse embryonic stem cells Hagiwara-Chatani, Natsumi; Shirai, Kota; Kido, Takumi Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/srep39868	journal	January 2017
Epimorphin expression in interstitial pneumonia Terasaki, Yasuhiro; Fukuda, Yuh; Suga, Moritaka Respiratory Research, Vol. 6, Issue 1 https://doi.org/10.1186/1465-9921-6-6	journal	January 2005
Myoepithelial cells: good fences make good neighbors Adriance, Melissa C.; Inman, Jamie L.; Petersen, Ole W. Breast Cancer Research, Vol. 7, Issue 5 https://doi.org/10.1186/bcr1286	journal	July 2005
Epimorphin Regulates Bile Duct Formation via Effects on Mitosis Orientation in Rat Liver Epithelial Stem-Like Cells Zhou, Junnian; Zhao, Lei; Qin, Lipeng PLoS ONE, Vol. 5, Issue 3 https://doi.org/10.1371/journal.pone.0009732	journal	March 2010
Regulation of Epithelial-Mesenchymal Transition in Breast Cancer Cells by Cell Contact and Adhesion Cichon, Magdalena A.; Nelson, Celeste M.; Radisky, Derek C. Cancer Informatics, Vol. 14s3 https://doi.org/10.4137/cin.s18965	journal	January 2015
Myoepithelial Cells in the Control of Mammary Development and Tumorigenesis: Data From Genetically Modified Mice Faraldo, Marisa M.; Teulière, Jérôme; Deugnier, Marie-Ange Journal of Mammary Gland Biology and Neoplasia, Vol. 10, Issue 3 https://doi.org/10.1007/s10911-005-9582-8	journal	July 2005
Polarized epithelial membrane traffic: conservation and plasticity Mostov, Keith; Su, Tao; ter Beest, Martin Nature Cell Biology, Vol. 5, Issue 4 https://doi.org/10.1038/ncb0403-287	journal	April 2003
Modelling glandular epithelial cancers in three-dimensional cultures Debnath, Jayanta; Brugge, Joan S. Nature Reviews Cancer, Vol. 5, Issue 9 https://doi.org/10.1038/nrc1695	journal	September 2005
CCAAT/enhancer binding protein beta (C/EBPβ)-2 transforms normal mammary epithelial cells and induces epithelial to mesenchymal transition in culture Bundy, L. M.; Sealy, L. Oncogene, Vol. 22, Issue 6 https://doi.org/10.1038/sj.onc.1206216	journal	February 2003
Cyclin D1 and C/EBPβ LAP1 Operate in a Common Pathway To Promote Mammary Epithelial Cell Differentiation Liu, Qiang; Boudot, Antoine; Ni, Jing Molecular and Cellular Biology, Vol. 34, Issue 16 https://doi.org/10.1128/mcb.00039-14	journal	August 2014
Morphogenic protein epimorphin protects intestinal epithelial cells from oxidative stress by the activation of EGF receptor and MEK/ERK, PI3 kinase/Akt signals Iizuka, Masahiro; Sasaki, Kenji; Hirai, Yohei American Journal of Physiology-Gastrointestinal and Liver Physiology, Vol. 292, Issue 1 https://doi.org/10.1152/ajpgi.00181.2006	journal	January 2007
Epimorphin expression in intestinal myofibroblasts induces epithelial morphogenesis Fritsch, Christine; Swietlicki, Elzbieta A.; Lefebvre, Olivier Journal of Clinical Investigation, Vol. 110, Issue 11 https://doi.org/10.1172/jci0213588	journal	December 2002

Similar Records

Epimorphin mediates mammary luminal morphogenesis through control of C/EBPbeta

Journal Article · Thu Mar 21 23:00:00 EST 2002 · The Journal of Cell Biology · OSTI ID:793806

Epimorphin Functions as a Key Morphoregulator for Mammary Epithelial Cells

Journal Article · Mon Oct 13 00:00:00 EDT 1997 · Journal of Cell Biology · OSTI ID:1003815

Epimorphin Functions as a Key Morphoregulator for Mammary Epithelial Cells

Journal Article · Sun Jan 11 23:00:00 EST 1998 · Journal of Cell Biology · OSTI ID:1625107

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CCAAT/enhancer binding protein
cell biology
epithelial–stromal interactions
mammary morphogenesis
transcription factor balance
transgenic mice

Epimorphin Mediates Mammary Luminal Morphogenesis through Control of C/EBPβ

Citation Formats

References (87)

Cited By (15)

Similar Records

Related Subjects