Fixed-target protein serial microcrystallography with an x-ray free electron laser

Hunter, Mark S.; Segelke, Brent; Messerschmidt, Marc; Williams, Garth J.; Zatsepin, Nadia A.; Barty, Anton; Benner, W. Henry; Carlson, David B.; Coleman, Matthew; Graf, Alexander; Hau-Riege, Stefan P.; Pardini, Tommaso; Seibert, M. Marvin; Evans, James; Boutet, Sébastien; Frank, Matthias

doi:10.1038/srep06026

Fixed-target protein serial microcrystallography with an x-ray free electron laser

Journal Article · Tue Aug 12 00:00:00 EDT 2014 · Scientific Reports

DOI:https://doi.org/10.1038/srep06026· OSTI ID:1624734

Hunter, Mark S. ^[1]; Segelke, Brent ^[2]; Messerschmidt, Marc ^[3]; Williams, Garth J. ^[3]; Zatsepin, Nadia A. ^[4]; Barty, Anton ^[5]; Benner, W. Henry ^[2]; Carlson, David B. ^[6]; Coleman, Matthew ^[7]; Graf, Alexander ^[8]; Hau-Riege, Stefan P. ^[8]; Pardini, Tommaso ^[8]; Seibert, M. Marvin ^[3]; Evans, James ^[9]; Boutet, Sébastien ^[3]; Frank, Matthias ^[8]

Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Physics Division; DOE/OSTI
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Biosciences and Biotechnology Division
SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source
Arizona State Univ., Tempe, AZ (United States). Dept. of Physics
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Centre for Free-Electron Laser Science
Univ. of California, Davis, CA (United States). Dept. of Molecular and Cellular Biology
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Biosciences and Biotechnology Division; Univ. of California, Davis, Sacramento, CA (United States). Medical Center. Dept. of Radiation Oncology
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Physics Division
Univ. of California, Davis, CA (United States). Dept. of Molecular and Cellular Biology; Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab.

We present results from experiments at the Linac Coherent Light Source (LCLS) demonstrating that serial femtosecond crystallography (SFX) can be performed to high resolution (,2.5 Å) using protein microcrystals deposited on an ultra-thin silicon nitride membrane and embedded in a preservation medium at room temperature. Data can be acquired at a high acquisition rate using x-ray free electron laser sources to overcome radiation damage, while sample consumption is dramatically reduced compared to flowing jet methods. We achieved a peak data acquisition rate of 10 Hz with a hit rate of ,38%, indicating that a complete data set could be acquired in about one 12-hour LCLS shift using the setup described here, or in even less time using hardware optimized for fixed target SFX. This demonstration opens the door to ultra low sample consumption SFX using the technique of diffraction-before-destruction on proteins that exist in only small quantities and/or do not produce the copious quantities of microcrystals required for flowing jet methods.

View Accepted Manuscript (DOE)

Research Organization:: Battelle Memorial Institute, Columbus, OH (United States); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF)

Grant/Contract Number:: AC05-76RL01830; AC52-07NA27344

OSTI ID:: 1624734

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 4; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

References (31)

X-ray Diffraction from Membrane Protein Nanocrystals Hunter, M. S.; DePonte, D. P.; Shapiro, D. A. Biophysical Journal, Vol. 100, Issue 1, p. 198-206 https://doi.org/10.1016/j.bpj.2010.10.049	journal	January 2011
Coherence properties of the European XFEL Geloni, G.; Saldin, E.; Samoylova, L. New Journal of Physics, Vol. 12, Issue 3 https://doi.org/10.1088/1367-2630/12/3/035021	journal	March 2010
The Protein Data Bank Berman, H. M. Nucleic Acids Research, Vol. 28, Issue 1 https://doi.org/10.1093/nar/28.1.235	journal	January 2000
Structure-factor analysis of femtosecond microdiffraction patterns from protein nanocrystals Kirian, Richard A.; White, Thomas A.; Holton, James M. Acta Crystallographica Section A Foundations of Crystallography, Vol. 67, Issue 2 https://doi.org/10.1107/s0108767310050981	journal	February 2011
Nanoflow electrospinning serial femtosecond crystallography Sierra, Raymond G.; Laksmono, Hartawan; Kern, Jan Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 11, p. 1584-1587 https://doi.org/10.1107/s0907444912038152	journal	October 2012
Cheetah : software for high-throughput reduction and analysis of serial femtosecond X-ray diffraction data Barty, Anton; Kirian, Richard A.; Maia, Filipe R. N. C. Journal of Applied Crystallography, Vol. 47, Issue 3 https://doi.org/10.1107/s1600576714007626	journal	May 2014
High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography Boutet, S.; Lomb, L.; Williams, G. J. Science, Vol. 337, Issue 6092 https://doi.org/10.1126/science.1217737	journal	May 2012
Natively Inhibited Trypanosoma brucei Cathepsin B Structure Determined by Using an X-ray Laser Redecke, L.; Nass, K.; DePonte, D. P. Science, Vol. 339, Issue 6116 https://doi.org/10.1126/science.1229663	journal	November 2012
Serial Femtosecond Crystallography of G Protein-Coupled Receptors Liu, W.; Wacker, D.; Gati, C. Science, Vol. 342, Issue 6165 https://doi.org/10.1126/science.1244142	journal	December 2013
Gas Dynamic Virtual Nozzle for Generation of Microscopic Droplet Streams DePonte, D. P.; Weierstall, U.; Starodub, D. arXiv https://doi.org/10.48550/arxiv.0803.4181	text	January 2008
Femtosecond X-ray protein nanocrystallography Chapman, Henry N.; Fromme, Petra; Barty, Anton Nature, Vol. 470, Issue 7332, p. 73-77 https://doi.org/10.1038/nature09750	journal	February 2011
Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography Weierstall, Uwe; James, Daniel; Wang, Chong Nature Communications, Vol. 5, Issue 1 https://doi.org/10.1038/ncomms4309	journal	February 2014
Determination of damage-free crystal structure of an X-ray–sensitive protein using an XFEL Hirata, Kunio; Shinzawa-Itoh, Kyoko; Yano, Naomine Nature Methods, Vol. 11, Issue 7 https://doi.org/10.1038/nmeth.2962	journal	May 2014
First lasing and operation of an ångstrom-wavelength free-electron laser Emma, P.; Akre, R.; Arthur, J. Nature Photonics, Vol. 4, Issue 9 https://doi.org/10.1038/nphoton.2010.176	journal	August 2010
The LCLS: A fourth generation light source using the SLAC linac Arthur, J.; Materlik, G.; Tatchyn, R. Review of Scientific Instruments, Vol. 66, Issue 2 https://doi.org/10.1063/1.1145778	journal	February 1995
Injector for scattering measurements on fully solvated biospecies Weierstall, U.; Spence, J. C. H.; Doak, R. B. Review of Scientific Instruments, Vol. 83, Issue 3 https://doi.org/10.1063/1.3693040	journal	March 2012
Monte Carlo study for optimal conditions in single-shot imaging with femtosecond x-ray laser pulses Park, Jaehyun; Joti, Yasumasa; Ishikawa, Tetsuya Applied Physics Letters, Vol. 103, Issue 26 https://doi.org/10.1063/1.4855635	journal	December 2013
Gas dynamic virtual nozzle for generation of microscopic droplet streams DePonte, D. P.; Weierstall, U.; Schmidt, K. Journal of Physics D: Applied Physics, Vol. 41, Issue 19, Article No. 195505 https://doi.org/10.1088/0022-3727/41/19/195505	journal	September 2008
X-ray lasers for structural and dynamic biology Spence, J. C. H.; Weierstall, U.; Chapman, H. N. Reports on Progress in Physics, Vol. 75, Issue 10 https://doi.org/10.1088/0034-4885/75/10/102601	journal	September 2012
The Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS) Boutet, Sébastien; J. Williams, Garth New Journal of Physics, Vol. 12, Issue 3 https://doi.org/10.1088/1367-2630/12/3/035024	journal	March 2010
CrystFEL : a software suite for snapshot serial crystallography White, Thomas A.; Kirian, Richard A.; Martin, Andrew V. Journal of Applied Crystallography, Vol. 45, Issue 2 https://doi.org/10.1107/S0021889812002312	journal	March 2012
Structure-factor analysis of femtosecond microdiffraction patterns from protein nanocrystals Kirian, Richard A.; White, Thomas A.; Holton, James M. Acta Crystallographica Section A Foundations of Crystallography, Vol. 67, Issue 2 https://doi.org/10.1107/S0108767310050981	journal	February 2011
Nanoflow electrospinning serial femtosecond crystallography Sierra, Raymond G.; Laksmono, Hartawan; Kern, Jan Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 11, p. 1584-1587 https://doi.org/10.1107/S0907444912038152	journal	October 2012
New Python-based methods for data processing Sauter, Nicholas K.; Hattne, Johan; Grosse-Kunstleve, Ralf W. Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 7 https://doi.org/10.1107/S0907444913000863	journal	June 2013
Cheetah : software for high-throughput reduction and analysis of serial femtosecond X-ray diffraction data Barty, Anton; Kirian, Richard A.; Maia, Filipe R. N. C. Journal of Applied Crystallography, Vol. 47, Issue 3 https://doi.org/10.1107/S1600576714007626	journal	May 2014
CrystFEL : a software suite for snapshot serial crystallography White, Thomas A.; Kirian, Richard A.; Martin, Andrew V. Journal of Applied Crystallography, Vol. 45, Issue 2 https://doi.org/10.1107/s0021889812002312	journal	March 2012
The Protein Data Bank Berman, Helen M.; Battistuz, Tammy; Bhat, T. N. Acta Crystallographica Section D Biological Crystallography, Vol. 58, Issue 6 https://doi.org/10.1107/s0907444902003451	journal	May 2002
New Python-based methods for data processing Sauter, Nicholas K.; Hattne, Johan; Grosse-Kunstleve, Ralf W. Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 7 https://doi.org/10.1107/s0907444913000863	journal	June 2013
The CSPAD megapixel x-ray camera at LCLS Hart, Philip; Boutet, Sébastien; Carini, Gabriella SPIE Optical Engineering + Applications, SPIE Proceedings https://doi.org/10.1117/12.930924	conference	October 2012
Femtosecond protein nanocrystallography—data analysis methods Kirian, Richard A.; Wang, Xiaoyu; Weierstall, Uwe Optics Express, Vol. 18, Issue 6 https://doi.org/10.1364/OE.18.005713	journal	January 2010
Femtosecond protein nanocrystallography—data analysis methods Kirian, Richard A.; Wang, Xiaoyu; Weierstall, Uwe Optics Express, Vol. 18, Issue 6 https://doi.org/10.1364/oe.18.005713	journal	January 2010

Cited By (72)

Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II Sierra, Raymond G.; Gati, Cornelius; Laksmono, Hartawan Nature Methods, Vol. 13, Issue 1 https://doi.org/10.1038/nmeth.3667	journal	November 2015
Polyacrylamide injection matrix for serial femtosecond crystallography Park, Jaehyun; Park, Sehan; Kim, Jangwoo Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-019-39020-9	journal	February 2019
Nylon mesh-based sample holder for fixed-target serial femtosecond crystallography Lee, Donghyeon; Baek, Sangwon; Park, Jaehyun Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-019-43485-z	journal	May 2019
A micro-patterned silicon chip as sample holder for macromolecular crystallography experiments with minimal background scattering Roedig, P.; Vartiainen, I.; Duman, R. Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep10451	journal	May 2015
Possibilities for serial femtosecond crystallography sample delivery at future light sourcesa) Chavas, L. M. G.; Gumprecht, L.; Chapman, H. N. Structural Dynamics, Vol. 2, Issue 4 https://doi.org/10.1063/1.4921220	journal	May 2015
Real-time investigation of dynamic protein crystallization in living cells Schönherr, R.; Klinge, M.; Rudolph, J. M. Structural Dynamics, Vol. 2, Issue 4 https://doi.org/10.1063/1.4921591	journal	July 2015
A shared vision for macromolecular crystallography over the next five years Förster, Andreas; Schulze-Briese, Clemens Structural Dynamics, Vol. 6, Issue 6 https://doi.org/10.1063/1.5131017	journal	November 2019
The crystal structure of dGTPase reveals the molecular basis of dGTP selectivity Barnes, Christopher O.; Wu, Ying; Song, Jinhu Proceedings of the National Academy of Sciences, Vol. 116, Issue 19 https://doi.org/10.1073/pnas.1814999116	journal	April 2019
Shortening injection matrix for serial crystallography Nam, Ki Hyun https://doi.org/10.1101/687640	posted_content	July 2019
Sample Delivery Media for Serial Crystallography Nam, Ki International Journal of Molecular Sciences, Vol. 20, Issue 5 https://doi.org/10.3390/ijms20051094	journal	March 2019
Perspective: Opportunities for ultrafast science at SwissFEL Abela, Rafael; Beaud, Paul; Van Bokhoven, Jeroen A. ETH Zurich https://doi.org/10.3929/ethz-b-000231826	text	January 2017
Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene Carolin, Seuring,; Kartik, Ayyer,; Eleftheria, Filippaki, ETH Zurich https://doi.org/10.3929/ethz-b-000265173	text	January 2018
Molecular dynamics simulations of macromolecular crystals Cerutti, David S.; Case, David A. Wiley Interdisciplinary Reviews: Computational Molecular Science, Vol. 9, Issue 4 https://doi.org/10.1002/wcms.1402	journal	November 2018
Climbing the Data Mountain: Processing of SFX Data Yoon, Chun Hong; White, Thomas A. X-ray Free Electron Lasers https://doi.org/10.1007/978-3-030-00551-1_7	book	January 2018
Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers Fuller, Franklin D.; Gul, Sheraz; Chatterjee, Ruchira Nature Methods, Vol. 14, Issue 4 https://doi.org/10.1038/nmeth.4195	journal	February 2017
High-speed fixed-target serial virus crystallography Roedig, Philip; Ginn, Helen M.; Pakendorf, Tim Nature Methods, Vol. 14, Issue 8 https://doi.org/10.1038/nmeth.4335	journal	June 2017
Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene Seuring, Carolin; Ayyer, Kartik; Filippaki, Eleftheria Nature Communications, Vol. 9, Article No. 1836 https://doi.org/10.1038/s41467-018-04116-9	journal	May 2018
Coherent diffractive imaging of microtubules using an X-ray laser Brändén, Gisela; Hammarin, Greger; Harimoorthy, Rajiv Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-10448-x	journal	June 2019
Shortening injection matrix for serial crystallography Nam, Ki Hyun Scientific Reports, Vol. 10, Issue 1 https://doi.org/10.1038/s41598-019-56135-1	journal	January 2020
Graphene-based microfluidics for serial crystallography Sui, Shuo; Wang, Yuxi; Kolewe, Kristopher W. Lab on a Chip, Vol. 16, Issue 16 https://doi.org/10.1039/c6lc00451b	journal	January 2016
Time-resolved structural studies with serial crystallography: A new light on retinal proteins Panneels, Valérie; Wu, Wenting; Tsai, Ching-Ju Structural Dynamics, Vol. 2, Issue 4 https://doi.org/10.1063/1.4922774	journal	June 2015
Microfluidics: From crystallization to serial time-resolved crystallography Sui, Shuo; Perry, Sarah L. Structural Dynamics, Vol. 4, Issue 3 https://doi.org/10.1063/1.4979640	journal	May 2017
Perspective: Opportunities for ultrafast science at SwissFEL Abela, Rafael; Beaud, Paul; van Bokhoven, Jeroen A. Structural Dynamics, Vol. 4, Issue 6 https://doi.org/10.1063/1.4997222	journal	November 2017
Serial Femtosecond Crystallography Chapman, Henry N. Synchrotron Radiation News, Vol. 28, Issue 6 https://doi.org/10.1080/08940886.2015.1101323	journal	November 2015
An outlook on using serial femtosecond crystallography in drug discovery Mishin, Alexey; Gusach, Anastasiia; Luginina, Aleksandra Expert Opinion on Drug Discovery, Vol. 14, Issue 9 https://doi.org/10.1080/17460441.2019.1626822	journal	May 2019
Fixed target single-shot imaging of nanostructures using thin solid membranes at SACLA Nam, Daewoong; Kim, Chan; Kim, Yoonhee Journal of Physics B: Atomic, Molecular and Optical Physics, Vol. 49, Issue 3 https://doi.org/10.1088/0953-4075/49/3/034008	journal	January 2016
Watching proteins function with time-resolved x-ray crystallography Šrajer, Vukica; Schmidt, Marius Journal of Physics D: Applied Physics, Vol. 50, Issue 37 https://doi.org/10.1088/1361-6463/aa7d32	journal	August 2017
Materials science in the time domain using Bragg coherent diffraction imaging Robinson, Ian; Clark, Jesse; Harder, Ross Journal of Optics, Vol. 18, Issue 5 https://doi.org/10.1088/2040-8978/18/5/054007	journal	March 2016
Sample delivery using capillary and delivery medium for serial crystallography Nam, Ki Hyun https://doi.org/10.1101/696138	posted_content	July 2019
Linac Coherent Light Source: The first five years Bostedt, Christoph; Boutet, Sébastien; Fritz, David M. Reviews of Modern Physics, Vol. 88, Issue 1 https://doi.org/10.1103/revmodphys.88.015007	journal	March 2016
Capture and X-ray diffraction studies of protein microcrystals in a microfluidic trap array Lyubimov, Artem Y.; Murray, Thomas D.; Koehl, Antoine Acta Crystallographica Section D Biological Crystallography, Vol. 71, Issue 4 https://doi.org/10.1107/s1399004715002308	journal	March 2015
Raster-scanning serial protein crystallography using micro- and nano-focused synchrotron beams Coquelle, Nicolas; Brewster, Aaron S.; Kapp, Ulrike Acta Crystallographica Section D Biological Crystallography, Vol. 71, Issue 5 https://doi.org/10.1107/s1399004715004514	journal	April 2015
In meso in situ serial X-ray crystallography of soluble and membrane proteins Huang, Chia-Ying; Olieric, Vincent; Ma, Pikyee Acta Crystallographica Section D Biological Crystallography, Vol. 71, Issue 6 https://doi.org/10.1107/s1399004715005210	journal	May 2015
A high-transparency, micro-patternable chip for X-ray diffraction analysis of microcrystals under native growth conditions Murray, Thomas D.; Lyubimov, Artem Y.; Ogata, Craig M. Acta Crystallographica Section D Biological Crystallography, Vol. 71, Issue 10 https://doi.org/10.1107/s1399004715015011	journal	September 2015
Fast two-dimensional grid and transmission X-ray microscopy scanning methods for visualizing and characterizing protein crystals Wojdyla, Justyna Aleksandra; Panepucci, Ezequiel; Martiel, Isabelle Journal of Applied Crystallography, Vol. 49, Issue 3 https://doi.org/10.1107/s1600576716006233	journal	May 2016
Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering Roedig, Philip; Duman, Ramona; Sanchez-Weatherby, Juan Journal of Applied Crystallography, Vol. 49, Issue 3 https://doi.org/10.1107/s1600576716006348	journal	May 2016
Direct protein crystallization on ultrathin membranes for diffraction measurements at X-ray free-electron lasers Opara, Nadia; Martiel, Isabelle; Arnold, Stefan A. Journal of Applied Crystallography, Vol. 50, Issue 3 https://doi.org/10.1107/s1600576717005799	journal	June 2017
3D printed droplet generation devices for serial femtosecond crystallography enabled by surface coating Echelmeier, Austin; Kim, Daihyun; Cruz Villarreal, Jorvani Journal of Applied Crystallography, Vol. 52, Issue 5 https://doi.org/10.1107/s1600576719010343	journal	August 2019
Successful sample preparation for serial crystallography experiments Beale, John H.; Bolton, Rachel; Marshall, Stephen A. Journal of Applied Crystallography, Vol. 52, Issue 6 https://doi.org/10.1107/s1600576719013517	journal	November 2019
Stable sample delivery in viscous media via a capillary for serial crystallography Nam, Ki Hyun Journal of Applied Crystallography, Vol. 53, Issue 1 https://doi.org/10.1107/s1600576719014985	journal	February 2020
The Coherent X-ray Imaging instrument at the Linac Coherent Light Source Liang, Mengning; Williams, Garth J.; Messerschmidt, Marc Journal of Synchrotron Radiation, Vol. 22, Issue 3 https://doi.org/10.1107/s160057751500449x	journal	April 2015
A modular and compact portable mini-endstation for high-precision, high-speed fixed target serial crystallography at FEL and synchrotron sources Sherrell, Darren A.; Foster, Andrew J.; Hudson, Lee Journal of Synchrotron Radiation, Vol. 22, Issue 6 https://doi.org/10.1107/s1600577515016938	journal	October 2015
DA+ data acquisition and analysis software at the Swiss Light Source macromolecular crystallography beamlines Wojdyla, Justyna Aleksandra; Kaminski, Jakub W.; Panepucci, Ezequiel Journal of Synchrotron Radiation, Vol. 25, Issue 1 https://doi.org/10.1107/s1600577517014503	journal	January 2018
Serial femtosecond crystallography: the first five years Schlichting, Ilme IUCrJ, Vol. 2, Issue 2, p. 246-255 https://doi.org/10.1107/s205225251402702x	journal	February 2015
Structure-factor amplitude reconstruction from serial femtosecond crystallography of two-dimensional membrane-protein crystals Casadei, Cecilia M.; Nass, Karol; Barty, Anton IUCrJ, Vol. 6, Issue 1 https://doi.org/10.1107/s2052252518014641	journal	January 2019
On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies Lieske, Julia; Cerv, Maximilian; Kreida, Stefan IUCrJ, Vol. 6, Issue 4 https://doi.org/10.1107/s2052252519007395	journal	June 2019
On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies Lieske, Julia; Cerv, Maximilian; Kreida, Stefan International Union of Crystallography (IUCr) https://doi.org/10.1107/s2052252519007395/mf5034sup1.pdf	text	January 2019
Toward G protein-coupled receptor structure-based drug design using X-ray lasers Ishchenko, Andrii; Stauch, Benjamin; Han, Gye Won IUCrJ, Vol. 6, Issue 6 https://doi.org/10.1107/s2052252519013137	journal	October 2019
A fixed-target platform for serial femtosecond crystallography in a hydrated environment Shelby, M. L.; Gilbile, D.; Grant, T. D. IUCrJ, Vol. 7, Issue 1 https://doi.org/10.1107/s2052252519014003	journal	January 2020
Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals Wolff, Alexander M.; Young, Iris D.; Sierra, Raymond G. IUCrJ, Vol. 7, Issue 2 https://doi.org/10.1107/s205225252000072x	journal	February 2020
In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures Huang, Chia-Ying; Olieric, Vincent; Ma, Pikyee Acta Crystallographica Section D Structural Biology, Vol. 72, Issue 1 https://doi.org/10.1107/s2059798315021683	journal	January 2016
Fixed target combined with spectral mapping: approaching 100% hit rates for serial crystallography Oghbaey, Saeed; Sarracini, Antoine; Ginn, Helen M. Acta Crystallographica Section D Structural Biology, Vol. 72, Issue 8, p. 944-955 https://doi.org/10.1107/s2059798316010834	journal	July 2016
Where is crystallography going? Grimes, Jonathan M.; Hall, David R.; Ashton, Alun W. Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 2 https://doi.org/10.1107/s2059798317016709	journal	February 2018
Crystallography on a chip – without the chip: sheet-on-sheet sandwich Doak, R. Bruce; Nass Kovacs, Gabriela; Gorel, Alexander Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 10 https://doi.org/10.1107/s2059798318011634	journal	October 2018
Sample delivery for serial crystallography at free-electron lasers and synchrotrons Grünbein, Marie Luise; Nass Kovacs, Gabriela Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2 https://doi.org/10.1107/s205979831801567x	journal	January 2019
Journey to the center of the protein: allostery from multitemperature multiconformer X-ray crystallography Keedy, Daniel A. Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2 https://doi.org/10.1107/s2059798318017941	journal	January 2019
Strategies for sample delivery for femtosecond crystallography Martiel, Isabelle; Müller-Werkmeister, Henrike M.; Cohen, Aina E. Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2 https://doi.org/10.1107/s2059798318017953	journal	February 2019
Strategies for sample delivery for femtosecond crystallography Martiel, Isabelle; Müller-Werkmeister, Henrike M.; Cohen, Aina E. International Union of Crystallography (IUCr) https://doi.org/10.1107/s2059798318017953/ba5296sup1.pdf	text	January 2019
A guide to sample delivery systems for serial crystallography Zhao, Feng‐Zhu; Zhang, Bin; Yan, Er‐Kai The FEBS Journal, Vol. 286, Issue 22 https://doi.org/10.1111/febs.15099	journal	August 2019
Time-resolved structural studies with serial crystallography: A new light on retinal proteins Panneels, Valérie; Wu, Wenting; Tsai, Ching-Ju Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2015-04290	text	January 2015
Serial Femtosecond Crystallography Chapman, Henry N. Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2015-05541	text	January 2015
Room-Temperature Macromolecular Crystallography Using a Micro-Patterned Silicon Chip with Minimal Background Scattering Roedig, Philip; Duman, Ramona; Sanchez-Weatherby, Juan Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2016-02690	text	January 2016
Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene Seuring, Carolin; Ayyer, Kartik; Filippaki, Eleftheria Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2018-01731	text	January 2018
Structure-factor amplitude reconstruction from serial femtosecond crystallography of two-dimensional membrane-protein crystals Casadei, Cecilia M.; Nass, Karol; Barty, Anton Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2018-04823	text	January 2019
Climbing the Data Mountain: Processing of SFX Data Yoon, Chun Hong; White, Thomas A. Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-00970	text	January 2018
On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies Lieske, Julia; Cerv, Maximilian; Kreida, Stefan Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-02620	text	January 2019
Coherent diffractive imaging of microtubules using an X-ray laser Brändén, Gisela; Hammarin, Greger; Harimoorthy, Rajiv Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-03073	text	January 2019
A fixed-target platform for serial femtosecond crystallography in a hydrated environment Shelby, M. L.; Gilbile, D.; Grant, T. D. Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-05316	text	January 2020
Cell-Free Co-Translational Approaches for Producing Mammalian Receptors: Expanding the Cell-Free Expression Toolbox Using Nanolipoproteins Shelby, Megan L.; He, Wei; Dang, Amanda T. Frontiers in Pharmacology, Vol. 10 https://doi.org/10.3389/fphar.2019.00744	journal	July 2019
A Graphene-Based Microfluidic Platform for Electrocrystallization and In Situ X-ray Diffraction Sui, Shuo; Wang, Yuxi; Dimitrakopoulos, Christos Crystals, Vol. 8, Issue 2 https://doi.org/10.3390/cryst8020076	journal	February 2018
Polysaccharide-Based Injection Matrix for Serial Crystallography Nam, Ki Hyun International Journal of Molecular Sciences, Vol. 21, Issue 9 https://doi.org/10.3390/ijms21093332	journal	May 2020
Perspective: Opportunities for ultrafast science at SwissFEL Abela, Rafael; Beaud, Paul; Van Bokhoven, Jeroen A. AIP Publishing https://doi.org/10.7892/boris.114739	text	January 2017

Similar Records

Nanoflow electrospinning serial femtosecond crystallography

Journal Article · Thu Nov 01 00:00:00 EDT 2012 · Acta Crystallographica. Section D: Biological Crystallography · OSTI ID:22351266

A novel inert crystal delivery medium for serial femtosecond crystallography

Journal Article · Tue Jun 30 00:00:00 EDT 2015 · IUCrJ · OSTI ID:1261172

A novel inert crystal delivery medium for serial femtosecond crystallography

Journal Article · Tue Jun 30 00:00:00 EDT 2015 · IUCrJ · OSTI ID:1208832

Related Subjects

77 NANOSCIENCE AND NANOTECHNOLOGY
nanocrystallography
science & technology
structure determination

Fixed-target protein serial microcrystallography with an x-ray free electron laser

Citation Formats

References (31)

Cited By (72)

Similar Records

Related Subjects