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Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser

Journal Article · · Scientific Data
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  1. Uppsala Univ. (Sweden). Dept. of Cell and Molecular Biology, Lab. of Molecular Biophysics; Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; DOE/OSTI
  2. Uppsala Univ. (Sweden). Dept. of Cell and Molecular Biology, Lab. of Molecular Biophysics
  3. Centre National de la Recherche Scientifique (CNRS) (France). Information Genomique et Structurale; Aix-Marseille Univ., Marseille (France). Inst. de Microbiologie de la Mediterranee, Parc Scientifique de Luminy
  4. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
  5. SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS); European X-ray Free-Electron Laser (XFEL), Hamburg (Germany)
  6. Uppsala Univ. (Sweden). Dept. of Cell and Molecular Biology, Lab. of Molecular Biophysics; Commissariat a l'Energie Atomique et aux Energies Alternatives (CEA), Saclay (France)
  7. Uppsala Univ. (Sweden). Dept. of Cell and Molecular Biology, Lab. of Molecular Biophysics; Uppsala Univ. (Sweden). Molekyl- och kondenserade materiens fysik, Institutionen för Fysik och Astronomi
  8. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
  9. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Univ. of Melbourne (Australia)
  10. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Photon Science
  11. SLAC National Accelerator Lab., Menlo Park, CA (United States). Photon Ultrafast Laser Science and Engineering Inst. (PULSE); National Univ. of Singapore (Singapore). Centre for BioImaging Sciences
  12. SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
  13. Synchrotron SOLEIL, Saint Aubin (France)
  14. SLAC National Accelerator Lab., Menlo Park, CA (United States). Photon Ultrafast Laser Science and Engineering Inst. (PULSE)
  15. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  16. Max Planck Inst. for Medical Research, Heidelberg (Germany)
  17. Max Planck Inst. for Medical Research, Heidelberg (Germany); Max Planck Advanced Study Group, Hamburg (Germany). Center for Free Electron Laser Science
  18. Max Planck Advanced Study Group, Hamburg (Germany). Center for Free Electron Laser Science; Max Planck Inst. fuer Kernphysik, Heidelberg (Germany)
  19. Max Planck Inst. for Medical Research, Heidelberg (Germany); Max Planck Advanced Study Group, Hamburg (Germany). Center for Free Electron Laser Science; Kansas State Univ., Manhattan, KS (United States). Dept. of Physics, J.R. Macdonald Lab.
  20. Max Planck Advanced Study Group, Hamburg (Germany). Center for Free Electron Laser Science; Max Planck Inst. fuer Kernphysik, Heidelberg (Germany); Kansas State Univ., Manhattan, KS (United States)Dept. of Physics, J.R. Macdonald Lab.
  21. PNSensor GmbH, Munich (Germany)
  22. Max Planck Inst. fuer Halbleiterlabor, Munich (Germany); Max Planck Inst. fuer Extraterrestrische Physik, Garching (Germany)
  23. PNSensor GmbH, Munich (Germany); Univ., Siegen (Germany)
  24. Uppsala Univ. (Sweden). Dept. of Cell and Molecular Biology, Lab. of Molecular Biophysics; Max Planck Inst. fuer Kernphysik, Heidelberg (Germany)
  25. Technische Univ. Berlin (Germany). Inst. fur Optik und Atomare Physik (IOAP)
  26. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Univ. of Hamburg (Germany)
  27. Uppsala Univ. (Sweden). Dept. of Cell and Molecular Biology, Lab. of Molecular Biophysics; European X-ray Free-Electron Laser (XFEL), Hamburg (Germany)
+ Show Author Affiliations

Free-electron lasers (FEL) hold the potential to revolutionize structural biology by producing X-ray pules short enough to outrun radiation damage, thus allowing imaging of biological samples without the limitation from radiation damage. Thus, a major part of the scientific case for the first FELs was three-dimensional (3D) reconstruction of non-crystalline biological objects. In a recent publication we demonstrated the first 3D reconstruction of a biological object from an X-ray FEL using this technique. The sample was the giant Mimivirus, which is one of the largest known viruses with a diameter of 450 nm. Here we present the dataset used for this successful reconstruction. Data-analysis methods for single-particle imaging at FELs are undergoing heavy development but data collection relies on very limited time available through a highly competitive proposal process. This dataset provides experimental data to the entire community and could boost algorithm development and provide a benchmark dataset for new algorithms.

Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES); Swedish Research Council; Knut and Alice Wallenberg Foundation; Max Planck Society; European Research Council; Röntgen-Ångström Cluster; Stiftelsen Olle Engkvist Byggmästare
Grant/Contract Number:
AC02-76SF00515
OSTI ID:
1624547
Journal Information:
Scientific Data, Journal Name: Scientific Data Journal Issue: 1 Vol. 3; ISSN 2052-4463
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (7)

Kinetics of Mimivirus Infection Stages Quantified Using Image Flow Cytometry journal April 2019
Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene text January 2018
Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene journal May 2018
The trickle before the torrent—diffraction data from X-ray lasers journal August 2016
Supervised classification methods for flash X-ray single particle diffraction imaging journal January 2019
Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene text January 2018
Biological single-particle imaging using XFELs – towards the next resolution revolution text January 2018

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59 BASIC BIOLOGICAL SCIENCES
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