Necessary Experimental Conditions for Single-Shot Diffraction Imaging of DNA-Based Structures with X-ray Free-Electron Lasers

Sun, Zhibin; Fan, Jiadong; Li, Haoyuan; Liu, Huajie; Nam, Daewoong; Kim, Chan; Kim, Yoonhee; Han, Yubo; Zhang, Jianhua; Yao, Shengkun; Park, Jaehyun; Kim, Sunam; Tono, Kensuke; Yabashi, Makina; Ishikawa, Tetsuya; Song, Changyong; Fan, Chunhai; Jiang, Huaidong

doi:10.1021/acsnano.8b01838

Title: Necessary Experimental Conditions for Single-Shot Diffraction Imaging of DNA-Based Structures with X-ray Free-Electron Lasers

Journal Article · Mon Jul 09 00:00:00 EDT 2018 · ACS Nano

DOI:https://doi.org/10.1021/acsnano.8b01838· OSTI ID:1476144

Sun, Zhibin ^[1]; Fan, Jiadong ^[2]; Li, Haoyuan ^[3]; Liu, Huajie ^[4]; Nam, Daewoong ^[5]; Kim, Chan ^[6]; Kim, Yoonhee ^[6]; Han, Yubo ^[7]; Zhang, Jianhua ^[8]; Yao, Shengkun ^[2]; Park, Jaehyun ^[5]; Kim, Sunam ^[5]; Tono, Kensuke ^[9]; Yabashi, Makina ^[10]; Ishikawa, Tetsuya ^[10]; Song, Changyong ^[5];

^[4];

^[2]

Shandong Univ., Jinan (China); SLAC National Accelerator Lab., Menlo Park, CA (United States); ShanghaiTech Univ., Shanghai (China)
ShanghaiTech Univ., Shanghai (China)
SLAC National Accelerator Lab., Menlo Park, CA (United States); Stanford Univ., Stanford, CA (United States)
Chinese Academy of Sciences, Shanghai (China)
Pohang Univ. of Science and Technology, Pohang (Korea)
European XFEL GmbH, Schenefeld (Germany); Gwangju Institute of Science and Technology, Gwangju (Korea)
Chinese Academy of Sciences (CAS), Beijing (China); SLAC National Accelerator Lab., Menlo Park, CA (United States)
Shandong Univ., Jinan (China); ShanghaiTech Univ., Shanghai (China)
Japan Synchrotron Radiation Research Institute, Hyogo (Japan)
RIKEN SPring-8 Center, Hyogo (Japan)

It has been proposed that the radiation damage to biological particles and soft condensed matter can be overcome by ultrafast and ultraintense X-ray free-electron lasers (FELs) with short pulse durations. The successful demonstration of the “diffraction-before-destruction” concept has made single-shot diffraction imaging a promising tool to achieve high resolutions under the native states of samples. However, the resolution is still limited because of the low signal-to-noise ratio, especially for biological specimens such as cells, viruses, and macromolecular particles. Here, we present a demonstration single-shot diffraction imaging experiment of DNA-based structures at SPring-8 Angstrom Compact Free Electron Laser (SACLA), Japan. Through quantitative analysis of the reconstructed images, the scattering abilities of gold and DNA were demonstrated. Suggestions for extracting valid DNA signals from noisy diffraction patterns were also explained and outlined. To sketch out the necessary experimental conditions for the 3D imaging of DNA origami or DNA macromolecular particles, we carried out numerical simulations with practical detector noise and experimental geometry using the Linac Coherent Light Source (LCLS) at the SLAC National Accelerator Laboratory, USA. In conclusion, the simulated results demonstrate that it is possible to capture images of DNA-based structures at high resolutions with the technique development of current and next-generation X-ray FEL facilities.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: 2017YFA0504802; 2014CB910401; AC02-76SF00515; 2016R1A2B3010980; 201606220148; 31430031; 21727817

OSTI ID:: 1476144

Journal Information:: ACS Nano, Vol. 12, Issue 8; ISSN 1936-0851

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 9 works

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