Extraction and biomolecular analysis of dermal interstitial fluid collected with hollow microneedles

Miller, Philip R.; Taylor, Robert M.; Tran, Bao Quoc; Boyd, Gabrielle; Glaros, Trevor; Chavez, Victor H.; Krishnakumar, Raga; Sinha, Anupama; Poorey, Kunal; Williams, Kelly P.; Branda, Steven S.; Baca, Justin T.; Polsky, Ronen

doi:10.1038/s42003-018-0170-z

Extraction and biomolecular analysis of dermal interstitial fluid collected with hollow microneedles

Journal Article · Mon Oct 22 00:00:00 EDT 2018 · Communications Biology

DOI:https://doi.org/10.1038/s42003-018-0170-z· OSTI ID:1624529

Miller, Philip R. ^[1]; Taylor, Robert M. ^[2]; Tran, Bao Quoc ^[3]; Boyd, Gabrielle ^[3]; Glaros, Trevor ^[4]; Chavez, Victor H. ^[5]; Krishnakumar, Raga ^[6]; Sinha, Anupama ^[6]; Poorey, Kunal ^[6]; ^[6]; Branda, Steven S. ^[6]; ^[2]; Polsky, Ronen ^[5]

Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); DOE/OSTI
Univ. of New Mexico, Albuquerque, NM (United States)
Excet Inc., Springfield, VA (United States)
US Army Edgewood Chemical Biological Center, Edgewood, MD (United States)
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sandia National Lab. (SNL-CA), Livermore, CA (United States)

Dermal interstitial fluid (ISF) is an underutilized information-rich biofluid potentially useful in health status monitoring applications whose contents remain challenging to characterize. Here, we present a facile microneedle approach for dermal ISF extraction with minimal pain and no blistering for human subjects and rats. Extracted ISF volumes were sufficient for determining transcriptome, and proteome signatures. We noted similar profiles in ISF, serum, and plasma samples, suggesting that ISF can be a proxy for direct blood sampling. Dynamic changes in RNA-seq were recorded in ISF from induced hypoxia conditions. Finally, we report the first isolation and characterization, to our knowledge, of exosomes from dermal ISF. The ISF exosome concentration is 12–13 times more enriched when compared to plasma and serum and represents a previously unexplored biofluid for exosome isolation. This minimally invasive extraction approach can enable mechanistic studies of ISF and demonstrates the potential of ISF for real-time health monitoring applications.

View Accepted Manuscript (DOE)

Research Organization:: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA); Defense Threat Reduction Agency (DTRA); Edgewood Chemical Biological Center (ECBC); National Institutes of Health (NIH)

Grant/Contract Number:: NA0003525

OSTI ID:: 1624529

Alternate ID(s):: OSTI ID: 1871985

Journal Information:: Communications Biology, Journal Name: Communications Biology Journal Issue: 1 Vol. 1; ISSN 2399-3642

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

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