Sequence-selective dynamic covalent assembly of information-bearing oligomers

Leguizamon, Samuel C.; Scott, Timothy F.

doi:10.1038/s41467-020-14607-3

Sequence-selective dynamic covalent assembly of information-bearing oligomers

Journal Article · Fri Feb 07 04:00:00 EST 2020 · Nature Communications

DOI:https://doi.org/10.1038/s41467-020-14607-3· OSTI ID:1624249

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Univ. of Michigan, Ann Arbor, MI (United States); DOE/OSTI
Univ. of Michigan, Ann Arbor, MI (United States); Monash Univ., Melbourne, VIC (Australia)

Relatively robust dynamic covalent interactions have been employed extensively to mediate molecular self-assembly reactions; however, these assembly processes often do not converge to a thermodynamic equilibrium, instead yielding mixtures of kinetically-trapped species. Here, we report a dynamic covalent self-assembly process that mitigates kinetic trapping such that multiple unique oligomers bearing covalently coreactive pendant groups are able to undergo simultaneous, sequence-selective hybridization with their complementary strands to afford biomimetic, in-registry molecular ladders with covalent rungs. Analogous to the thermal cycling commonly employed for nucleic acid melting and annealing, this is achieved by raising and lowering the concentration of a multi-role reagent to effect quantitative dissociation and subsequently catalyze covalent bond rearrangement, affording selective assembly of the oligomeric sequences. The hybridization specificity afforded by this process further enabled information encoded in oligomers to be retrieved through selective hybridization with complementary, mass-labeled sequences.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Michigan, Ann Arbor, MI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF); National Institutes of Health (NIH)

Grant/Contract Number:: SC0012479

OSTI ID:: 1624249

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 11; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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