Structure and immunogenicity of a stabilized HIV-1 envelope trimer based on a group-M consensus sequence

Sliepen, Kwinten; Han, Byung Woo; Bontjer, Ilja; Mooij, Petra; Garces, Fernando; Behrens, Anna-Janina; Rantalainen, Kimmo; Kumar, Sonu; Sarkar, Anita; Brouwer, Philip J.  M.; Hua, Yuanzi; Tolazzi, Monica; Schermer, Edith; Torres, Jonathan L.; Ozorowski, Gabriel; van der Woude, Patricia; de la Peña, Alba Torrents; van Breemen, Mariëlle J.; Camacho-Sánchez, Juan Miguel; Burger, Judith A.; Medina-Ramírez, Max; González, Nuria; Alcami, Jose; LaBranche, Celia; Scarlatti, Gabriella; van Gils, Marit J.; Crispin, Max; Montefiori, David C.; Ward, Andrew B.; Koopman, Gerrit; Moore, John P.; Shattock, Robin J.; Bogers, Willy M.; Wilson, Ian A.; Sanders, Rogier W.

doi:10.1038/s41467-019-10262-5

Structure and immunogenicity of a stabilized HIV-1 envelope trimer based on a group-M consensus sequence

Journal Article · Wed May 29 00:00:00 EDT 2019 · Nature Communications

DOI:https://doi.org/10.1038/s41467-019-10262-5· OSTI ID:1624156

^[1]; ^[2]; Bontjer, Ilja ^[3]; Mooij, Petra ^[4]; Garces, Fernando ^[5]; Behrens, Anna-Janina ^[6]; Rantalainen, Kimmo ^[7]; ^[7]; ^[7]; Brouwer, Philip J. M. ^[3]; Hua, Yuanzi ^[8]; Tolazzi, Monica ^[9]; Schermer, Edith ^[3]; ^[8]; ^[8]; van der Woude, Patricia ^[3]; de la Peña, Alba Torrents ^[3]; van Breemen, Mariëlle J. ^[3]; ^[3]; Burger, Judith A. ^[3] more »

Univ. of Amsterdam (Netherlands). Dept. of Medical Microbiology, Amsterdam Infection & Immunity Institute; DOE/OSTI
The Scripps Research Insttute, La Jolla, CA (United States). Dept. of Integrative Structural and Computational Biology, Scripps CHAVI-ID, IAVI Neutralizing Antibody Center and Collaboration for AIDS Vaccine Discovery (CAVD); Seoul National Univ. (Korea, Republic of). Research Institute of Pharmaceutical Sciences, College of Pharmacy
Univ. of Amsterdam (Netherlands). Dept. of Medical Microbiology, Amsterdam Infection & Immunity Institute
Biomedical Primate Research Centre, Rijswijk (Netherlands). Dept. of Virology
The Scripps Research Insttute, La Jolla, CA (United States). Dept. of Integrative Structural and Computational Biology, Scripps CHAVI-ID, IAVI Neutralizing Antibody Center and Collaboration for AIDS Vaccine Discovery (CAVD); Amgen Inc., Thousand Oaks, CA (United States). Dept. of Therapeutics Discovery
Univ. of Oxford (United Kingdom). Oxford Glycobiology Institute, Department of Biochemistry; New England Biolabs Inc., Ipswich, MA (United States)
The Scripps Research Insttute, La Jolla, CA (United States). Dept. of Integrative Structural and Computational Biology, Scripps CHAVI-ID, IAVI Neutralizing Antibody Center and Collaboration for AIDS Vaccine Discovery (CAVD)
The Scripps Research Insttute, La Jolla, CA (United States). Dept. of Integrative Structural and Computational Biology, Scripps CHAVI-ID, IAVI Neutralizing Antibody Center and Collaboration for AIDS Vaccine Discovery (CAVD)
IRCCS San Raffaele Scientific Institute, Milan (Italy). Viral Evolution and Transmission Unit, Division of Immunology, Transplantation and Infectious Diseases
Carlos III Health Inst., Madrid (Spain). AIDS Immunopathology Unit
Duke Univ., Durham, NC (United States). Medical Center, Dept. of Surgery
IRCCS San Raffaele Scientific Institute, Milan (Italy). Viral Evolution and Transmission Unit, Division of Immunology, Transplantation and Infectious Diseases
Univ. of Oxford (United Kingdom). Dept. of Biochemistry, Oxford Glycobiology Institute; Univ. of Southampton (United Kingdom). Centre for Biological Sciences and Inst. for Life Sciences
Cornell Univ., Ithaca, NY (United States). Weill Medical College, Dept. of Microbiology and Immunology
Imperial College, London (United Kingdom). Section of Virology, Division of Infectious Diseases, Dept. of Medicine
Biomedical Primate Research Centre, Rijswijk (Netherlands). Dept. of Virology
The Scripps Research Insttute, La Jolla, CA (United States). Dept. of Integrative Structural and Computational Biology, Scripps CHAVI-ID, IAVI Neutralizing Antibody Center and Collaboration for AIDS Vaccine Discovery (CAVD); Scripps Research Institute, San Diego, CA (United States). Skaggs Inst. for Chemical Biology
Univ. of Amsterdam (Netherlands). Dept. of Medical Microbiology, Amsterdam Infection & Immunity Institute; Cornell Univ., Ithaca, NY (United States). Weill Medical College, Dept. of Microbiology and Immunology

Stabilized HIV-1 envelope glycoproteins (Env) that resemble the native Env are utilized in vaccination strategies aimed at inducing broadly neutralizing antibodies (bNAbs). To limit the exposure of rare isolate-specific antigenic residues/determinants we generated a SOSIP trimer based on a consensus sequence of all HIV-1 group M isolates (ConM). The ConM trimer displays the epitopes of most known bNAbs and several germline bNAb precursors. The crystal structure of the ConM trimer at 3.9Å resolution resembles that of the native Env trimer and its antigenic surface displays few rare residues. The ConM trimer elicits strong NAb responses against the autologous virus in rabbits and macaques that are significantly enhanced when it is presented on ferritin nanoparticles. The dominant NAb specificity is directed against an epitope at or close to the trimer apex. Immunogens based on consensus sequences might have utility in engineering vaccines against HIV-1 and other viruses.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: Bill and Melinda Gates Foundation; European Research Council (ERC); National Institutes of Health (NIH); National Research Foundation of Korea (NRF); USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1624156

Alternate ID(s):: OSTI ID: 1847725

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 10; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cell- and Protein-Directed Glycosylation of Native Cleaved HIV-1 Envelope Pritchard, Laura K.; Harvey, David J.; Bonomelli, Camille Journal of Virology, Vol. 89, Issue 17 https://doi.org/10.1128/JVI.01190-15	journal	June 2015
Influences on Trimerization and Aggregation of Soluble, Cleaved HIV-1 SOSIP Envelope Glycoprotein Klasse, P. J.; Depetris, R. S.; Pejchal, R. Journal of Virology, Vol. 87, Issue 17, p. 9873-9885 https://doi.org/10.1128/JVI.01226-13	journal	July 2013
HIV-1 Escape from a Peptidic Anchor Inhibitor through Stabilization of the Envelope Glycoprotein Spike Eggink, Dirk; de Taeye, Steven W.; Bontjer, Ilja Journal of Virology, Vol. 90, Issue 23 https://doi.org/10.1128/JVI.01616-16	journal	September 2016
Murine Antibody Responses to Cleaved Soluble HIV-1 Envelope Trimers Are Highly Restricted in Specificity Hu, Joyce K.; Crampton, Jordan C.; Cupo, Albert Journal of Virology, Vol. 89, Issue 20 https://doi.org/10.1128/JVI.01653-15	journal	August 2015
Immunogenicity in Rabbits of HIV-1 SOSIP Trimers from Clades A, B, and C, Given Individually, Sequentially, or in Combination Torrents de la Peña, Alba; de Taeye, Steven W.; Sliepen, Kwinten Journal of Virology, Vol. 92, Issue 8 https://doi.org/10.1128/JVI.01957-17	journal	January 2018
Tiered Categorization of a Diverse Panel of HIV-1 Env Pseudoviruses for Assessment of Neutralizing Antibodies Seaman, M. S.; Janes, H.; Hawkins, N. Journal of Virology, Vol. 84, Issue 3 https://doi.org/10.1128/JVI.02108-09	journal	November 2009
Antigenicity and Immunogenicity of Transmitted/Founder, Consensus, and Chronic Envelope Glycoproteins of Human Immunodeficiency Virus Type 1 Liao, H. -X.; Tsao, C. -Y.; Alam, S. M. Journal of Virology, Vol. 87, Issue 8 https://doi.org/10.1128/JVI.02297-12	journal	January 2013
Structure-Guided Redesign Increases the Propensity of HIV Env To Generate Highly Stable Soluble Trimers Guenaga, Javier; Dubrovskaya, Viktoriya; de Val, Natalia Journal of Virology, Vol. 90, Issue 6 https://doi.org/10.1128/JVI.02652-15	journal	December 2015
Diverse Recombinant HIV-1 Envs Fail To Activate B Cells Expressing the Germline B Cell Receptors of the Broadly Neutralizing Anti-HIV-1 Antibodies PG9 and 447-52D McGuire, A. T.; Glenn, J. A.; Lippy, A. Journal of Virology, Vol. 88, Issue 5 https://doi.org/10.1128/JVI.03228-13	journal	December 2013
A Native-Like SOSIP.664 Trimer Based on an HIV-1 Subtype B env Gene Pugach, Pavel; Ozorowski, Gabriel; Cupo, Albert Journal of Virology, Vol. 89, Issue 6 https://doi.org/10.1128/JVI.03473-14	journal	January 2015
A Recombinant Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Complex Stabilized by an Intermolecular Disulfide Bond between the gp120 and gp41 Subunits Is an Antigenic Mimic of the Trimeric Virion-Associated Structure Binley, J. M.; Sanders, R. W.; Clas, B. Journal of Virology, Vol. 74, Issue 2 https://doi.org/10.1128/JVI.74.2.627-643.2000	journal	January 2000
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Enhancing the Proteolytic Maturation of Human Immunodeficiency Virus Type 1 Envelope Glycoproteins Binley, James M.; Sanders, Rogier W.; Master, Aditi Journal of Virology, Vol. 76, Issue 6 https://doi.org/10.1128/JVI.76.6.2606-2616.2002	journal	March 2002
Rhesus macaques vaccinated with consensus envelopes elicit partially protective immune responses against SHIV SF162p4 challenge Eugene, Hermancia S.; Pierce-Paul, Brooke R.; Cragio, Jodi K. Virology Journal, Vol. 10, Issue 1 https://doi.org/10.1186/1743-422X-10-102	journal	January 2013
Stabilizing HIV-1 envelope glycoprotein trimers to induce neutralizing antibodies Torrents de la Peña, Alba; Sanders, Rogier W. Retrovirology, Vol. 15, Issue 1 https://doi.org/10.1186/s12977-018-0445-y	journal	September 2018
HIV-1 envelope glycoprotein immunogens to induce broadly neutralizing antibodies Sliepen, Kwinten; Sanders, Rogier W. Expert Review of Vaccines, Vol. 15, Issue 3 https://doi.org/10.1586/14760584.2016.1129905	journal	December 2015
Structure and immune recognition of trimeric pre-fusion HIV-1 Env B., Munro, James; Lynn, Morris,; Tongqing, Zhou, The University of North Carolina at Chapel Hill University Libraries https://doi.org/10.17615/bt86-bx50	text	January 2014
Antigenicity and Immunogenicity of Transmitted/Founder, Consensus, and Chronic Envelope Glycoproteins of Human Immunodeficiency Virus Type 1 H., Chen,; C., Montefiori, D.; M., Alam, S. The University of North Carolina at Chapel Hill University Libraries https://doi.org/10.17615/hryh-1381	text	January 2013
Recombinant HIV Envelope Proteins Fail to Engage Germline Versions of Anti-CD4bs bNAbs Hoot, Sam; McGuire, Andrew T.; Cohen, Kristen W. Public Library of Science (PLoS) https://doi.org/10.5167/uzh-110380	text	January 2013

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