Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates

Cottrell, Christopher A.; van Schooten, Jelle; Bowman, Charles A.; Yuan, Meng; Oyen, David; Shin, Mia; Morpurgo, Robert; van der Woude, Patricia; van Breemen, Mariëlle; Torres, Jonathan L.; Patel, Raj; Gross, Justin; Sewall, Leigh M.; Copps, Jeffrey; Ozorowski, Gabriel; Nogal, Bartek; Sok, Devin; Rakasz, Eva G.; Labranche, Celia; Vigdorovich, Vladimir; Christley, Scott; Carnathan, Diane G.; Sather, D. Noah; Montefiori, David; Silvestri, Guido; Burton, Dennis R.; Moore, John P.; Wilson, Ian A.; Sanders, Rogier W.; Ward, Andrew B.; van Gils, Marit J.

doi:10.1371/journal.ppat.1008753

Title: Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates

Journal Article · Mon Aug 31 00:00:00 EDT 2020 · PLoS Pathogens

DOI:https://doi.org/10.1371/journal.ppat.1008753· OSTI ID:1903966

^[1];

^[2];

^[1]; Yuan, Meng ^[1]; Oyen, David ^[1];

^[1];

^[2]; van der Woude, Patricia ^[2]; van Breemen, Mariëlle ^[2];

^[1]; Patel, Raj ^[1];

^[1];

^[1]; Rakasz, Eva G. ^[3];

^[4];

^[5] more »

The Scripps Research Institute, La Jolla, CA (United States)
University of Amsterdam (Netherlands)
University of Wisconsin, Madison, WI (United States)
Duke University, Durham, NC (United States). Medical Center
Seattle Children’s Research Institute, Seattle, WA (United States)
University of Texas Southwestern Medical Center, Dallas, TX (United States)
Scripps Research Institute, San Diego, CA (United States); Emory University, Atlanta, GA (United States)
Seattle Children’s Research Institute, Seattle, Washington, United States
The Scripps Research Institute, La Jolla, CA (United States); Emory University, Atlanta, GA (United States)
Massachusetts Institute of Technology (MIT), Cambridge, MA (United States); Harvard University, Cambridge, MA (United States)
University of Amsterdam (Netherlands); Cornell University, Ithaca, NY (United States)

The induction of broad and potent immunity by vaccines is the key focus of research efforts aimed at protecting against HIV-1 infection. Soluble native-like HIV-1 envelope glycoproteins have shown promise as vaccine candidates as they can induce potent autologous neutralizing responses in rabbits and non-human primates. In this study, monoclonal antibodies were isolated and characterized from rhesus macaques immunized with the BG505 SOSIP.664 trimer to better understand vaccine-induced antibody responses. Our studies reveal a diverse landscape of antibodies recognizing immunodominant strain-specific epitopes and non-neutralizing neo-epitopes. Additionally, we isolated a subset of mAbs against an epitope cluster at the gp120-gp41 interface that recognize the highly conserved fusion peptide and the glycan at position 88 and have characteristics akin to several human-derived broadly neutralizing antibodies.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: SLAC National Accelerator Laboratory, Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL); The Scripps Research Inst., La Jolla, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH); Bill and Melinda Gates Foundation; European Union (EU); Ruth L. Kirschstein Predoctoral Award; College Scientists Foundation; Netherlands Organization for Scientific Research (NWO)

Grant/Contract Number:: AC02-76SF00515; P41GM103393; P01 AI110657; UM1 AI44462; R01 AI13082; OPP1115782; OPP1132237; OPP1084519; OPP119635; 681137; Al131873; 109718-63-RKNT; 109514-61-RKVA; S10OD021634

OSTI ID:: 1903966

Journal Information:: PLoS Pathogens, Vol. 16, Issue 8; ISSN 1553-7374

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

References (73)

Structure and immune recognition of trimeric pre-fusion HIV-1 Env Pancera, Marie; Zhou, Tongqing; Druz, Aliaksandr Nature, Vol. 514, Issue 7523 https://doi.org/10.1038/nature13808	journal	October 2014
A new rhesus macaque assembly and annotation for next-generation sequencing analyses Zimin, Aleksey V.; Cornish, Adam S.; Maudhoo, Mnirnal D. Biology Direct, Vol. 9, Issue 1 https://doi.org/10.1186/1745-6150-9-20	journal	October 2014
Clustal Omega for making accurate alignments of many protein sequences: Clustal Omega for Many Protein Sequences Sievers, Fabian; Higgins, Desmond G. Protein Science, Vol. 27, Issue 1 https://doi.org/10.1002/pro.3290	journal	October 2017
Two-Component Ferritin Nanoparticles for Multimerization of Diverse Trimeric Antigens Georgiev, Ivelin S.; Joyce, Michael Gordon; Chen, Rita E. ACS Infectious Diseases, Vol. 4, Issue 5 https://doi.org/10.1021/acsinfecdis.7b00192	journal	February 2018
Automated analysis of high-throughput B-cell sequencing data reveals a high frequency of novel immunoglobulin V gene segment alleles Gadala-Maria, Daniel; Yaari, Gur; Uduman, Mohamed Proceedings of the National Academy of Sciences, Vol. 112, Issue 8 https://doi.org/10.1073/pnas.1417683112	journal	February 2015
Vaccination with Glycan-Modified HIV NFL Envelope Trimer-Liposomes Elicits Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability Dubrovskaya, Viktoriya; Tran, Karen; Ozorowski, Gabriel Immunity, Vol. 51, Issue 5 https://doi.org/10.1016/j.immuni.2019.10.008	journal	November 2019
Neutralizing antibody affords comparable protection against vaginal and rectal simian/human immunodeficiency virus challenge in macaques Moldt, Brian; Le, Khoa M.; Carnathan, Diane G. AIDS, Vol. 30, Issue 10 https://doi.org/10.1097/QAD.0000000000001102	journal	June 2016
Broadly Neutralizing Antibody PGT121 Allosterically Modulates CD4 Binding via Recognition of the HIV-1 gp120 V3 Base and Multiple Surrounding Glycans Julien, Jean-Philippe; Sok, Devin; Khayat, Reza PLoS Pathogens, Vol. 9, Issue 5 https://doi.org/10.1371/journal.ppat.1003342	journal	May 2013
Broadly Neutralizing Antibodies to HIV and Their Role in Vaccine Design Burton, Dennis R.; Hangartner, Lars Annual Review of Immunology, Vol. 34, Issue 1 https://doi.org/10.1146/annurev-immunol-041015-055515	journal	May 2016
Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer Cao, Liwei; Pauthner, Matthias; Andrabi, Raiees Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-018-06121-4	journal	September 2018
Vaccine-Induced Protection from Homologous Tier 2 SHIV Challenge in Nonhuman Primates Depends on Serum-Neutralizing Antibody Titers Pauthner, Matthias G.; Nkolola, Joseph P.; Havenar-Daughton, Colin Immunity, Vol. 50, Issue 1 https://doi.org/10.1016/j.immuni.2018.11.011	journal	January 2019
The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling Arnold, Konstantin; Bordoli, Lorenza; Kopp, Jürgen Bioinformatics, Vol. 22, Issue 2 https://doi.org/10.1093/bioinformatics/bti770	journal	November 2005
Production of individualized V gene databases reveals high levels of immunoglobulin genetic diversity Corcoran, Martin M.; Phad, Ganesh E.; Bernat, Néstor Vázquez Nature Communications, Vol. 7, Issue 1 https://doi.org/10.1038/ncomms13642	journal	December 2016
Automated molecular microscopy: The new Leginon system Suloway, Christian; Pulokas, James; Fellmann, Denis Journal of Structural Biology, Vol. 151, Issue 1 https://doi.org/10.1016/j.jsb.2005.03.010	journal	July 2005
MolProbity: More and better reference data for improved all-atom structure validation: PROTEIN SCIENCE.ORG Williams, Christopher J.; Headd, Jeffrey J.; Moriarty, Nigel W. Protein Science, Vol. 27, Issue 1 https://doi.org/10.1002/pro.3330	journal	November 2017
The HIV-1 Envelope Glycoprotein C3/V4 Region Defines a Prevalent Neutralization Epitope following Immunization Lei, Lin; Yang, Yuhe R.; Tran, Karen Cell Reports, Vol. 27, Issue 2 https://doi.org/10.1016/j.celrep.2019.03.039	journal	April 2019
Epitope-based vaccine design yields fusion peptide-directed antibodies that neutralize diverse strains of HIV-1 Xu, Kai; Acharya, Priyamvada; Kong, Rui Nature Medicine, Vol. 24, Issue 6 https://doi.org/10.1038/s41591-018-0042-6	journal	June 2018
A Next-Generation Cleaved, Soluble HIV-1 Env Trimer, BG505 SOSIP.664 gp140, Expresses Multiple Epitopes for Broadly Neutralizing but Not Non-Neutralizing Antibodies Sanders, Rogier W.; Derking, Ronald; Cupo, Albert PLoS Pathogens, Vol. 9, Issue 9, Article No. e1003618 https://doi.org/10.1371/journal.ppat.1003618	journal	September 2013
HIV-1 Cross-Reactive Primary Virus Neutralizing Antibody Response Elicited by Immunization in Nonhuman Primates Wang, Yimeng; O'Dell, Sijy; Turner, Hannah L. Journal of Virology, Vol. 91, Issue 21 https://doi.org/10.1128/JVI.00910-17	journal	August 2017
Crystal Structure of a Soluble Cleaved HIV-1 Envelope Trimer Julien, J. -P.; Cupo, A.; Sok, D. Science, Vol. 342, Issue 6165 https://doi.org/10.1126/science.1245625	journal	October 2013
Cryo-EM Structure of a Fully Glycosylated Soluble Cleaved HIV-1 Envelope Trimer Lyumkis, D.; Julien, J. -P.; de Val, N. Science, Vol. 342, Issue 6165 https://doi.org/10.1126/science.1245627	journal	October 2013
Quantitative analysis of cryo-EM density map segmentation by watershed and scale-space filtering, and fitting of structures by alignment to regions Pintilie, Grigore D.; Zhang, Junjie; Goddard, Thomas D. Journal of Structural Biology, Vol. 170, Issue 3 https://doi.org/10.1016/j.jsb.2010.03.007	journal	June 2010
Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization Kong, Rui; Duan, Hongying; Sheng, Zizhang Cell, Vol. 178, Issue 3 https://doi.org/10.1016/j.cell.2019.06.030	journal	July 2019
Recombinant HIV envelope trimer selects for quaternary-dependent antibodies targeting the trimer apex Sok, Devin; van Gils, Marit J.; Pauthner, Matthias Proceedings of the National Academy of Sciences, Vol. 111, Issue 49 https://doi.org/10.1073/pnas.1415789111	journal	November 2014
New tools for automated high-resolution cryo-EM structure determination in RELION-3 Zivanov, Jasenko; Nakane, Takanori; Forsberg, Björn O. eLife, Vol. 7 https://doi.org/10.7554/eLife.42166	journal	November 2018
Site-Specific Glycosylation of Virion-Derived HIV-1 Env Is Mimicked by a Soluble Trimeric Immunogen Struwe, Weston B.; Chertova, Elena; Allen, Joel D. Cell Reports, Vol. 24, Issue 8 https://doi.org/10.1016/j.celrep.2018.07.080	journal	August 2018
MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy Zheng, Shawn Q.; Palovcak, Eugene; Armache, Jean-Paul Nature Methods, Vol. 14, Issue 4 https://doi.org/10.1038/nmeth.4193	journal	February 2017
UCSF Chimera?A visualization system for exploratory research and analysis Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C. Journal of Computational Chemistry, Vol. 25, Issue 13 https://doi.org/10.1002/jcc.20084	journal	January 2004
Conformational Plasticity in the HIV-1 Fusion Peptide Facilitates Recognition by Broadly Neutralizing Antibodies Yuan, Meng; Cottrell, Christopher A.; Ozorowski, Gabriel Cell Host & Microbe, Vol. 25, Issue 6 https://doi.org/10.1016/j.chom.2019.04.011	journal	June 2019
An HIV-1 antibody from an elite neutralizer implicates the fusion peptide as a site of vulnerability van Gils, Marit J.; van den Kerkhof, Tom L. G. M.; Ozorowski, Gabriel Nature Microbiology, Vol. 2, Issue 2 https://doi.org/10.1038/nmicrobiol.2016.199	journal	November 2016
Structure-Guided Redesign Improves NFL HIV Env Trimer Integrity and Identifies an Inter-Protomer Disulfide Permitting Post-Expression Cleavage Yang, Lifei; Sharma, Shailendra Kumar; Cottrell, Christopher Frontiers in Immunology, Vol. 9 https://doi.org/10.3389/fimmu.2018.01631	journal	July 2018
HIV-1 neutralizing antibodies induced by native-like envelope trimers Sanders, R. W.; van Gils, M. J.; Derking, R. Science, Vol. 349, Issue 6244 https://doi.org/10.1126/science.aac4223	journal	June 2015
Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques Escolano, Amelia; Gristick, Harry B.; Abernathy, Morgan E. Nature, Vol. 570, Issue 7762 https://doi.org/10.1038/s41586-019-1250-z	journal	May 2019
Gctf: Real-time CTF determination and correction Zhang, Kai Journal of Structural Biology, Vol. 193, Issue 1 https://doi.org/10.1016/j.jsb.2015.11.003	journal	January 2016
Repertoire comparison of the B-cell receptor-encoding loci in humans and rhesus macaques by next-generation sequencing Vigdorovich, Vladimir; Oliver, Brian G.; Carbonetti, Sara Clinical & Translational Immunology, Vol. 5, Issue 7 https://doi.org/10.1038/cti.2016.42	journal	July 2016
HIV-1 broadly neutralizing antibody precursor B cells revealed by germline-targeting immunogen Jardine, J. G.; Kulp, D. W.; Havenar-Daughton, C. Science, Vol. 351, Issue 6280 https://doi.org/10.1126/science.aad9195	journal	March 2016
Electron-Microscopy-Based Epitope Mapping Defines Specificities of Polyclonal Antibodies Elicited during HIV-1 BG505 Envelope Trimer Immunization Bianchi, Matteo; Turner, Hannah L.; Nogal, Bartek Immunity, Vol. 49, Issue 2 https://doi.org/10.1016/j.immuni.2018.07.009	journal	August 2018
Holes in the Glycan Shield of the Native HIV Envelope Are a Target of Trimer-Elicited Neutralizing Antibodies McCoy, Laura E.; van Gils, Marit J.; Ozorowski, Gabriel Cell Reports, Vol. 16, Issue 9 https://doi.org/10.1016/j.celrep.2016.07.074	journal	August 2016
Structure and Diversity of the Rhesus Macaque Immunoglobulin Loci through Multiple De Novo Genome Assemblies Ramesh, Akshaya; Darko, Sam; Hua, Axin Frontiers in Immunology, Vol. 8 https://doi.org/10.3389/fimmu.2017.01407	journal	October 2017
Improving the Immunogenicity of Native-like HIV-1 Envelope Trimers by Hyperstabilization Torrents de la Peña, Alba; Julien, Jean-Philippe; de Taeye, Steven W. Cell Reports, Vol. 20, Issue 8 https://doi.org/10.1016/j.celrep.2017.07.077	journal	August 2017
Epitopes for neutralizing antibodies induced by HIV-1 envelope glycoprotein BG505 SOSIP trimers in rabbits and macaques Klasse, P. J.; Ketas, Thomas J.; Cottrell, Christopher A. PLOS Pathogens, Vol. 14, Issue 2 https://doi.org/10.1371/journal.ppat.1006913	journal	February 2018
Structure-based design of native-like HIV-1 envelope trimers to silence non-neutralizing epitopes and eliminate CD4 binding Kulp, Daniel W.; Steichen, Jon M.; Pauthner, Matthias Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/s41467-017-01549-6	journal	November 2017
Privateer: software for the conformational validation of carbohydrate structures Agirre, Jon; Iglesias-Fernández, Javier; Rovira, Carme Nature Structural & Molecular Biology, Vol. 22, Issue 11 https://doi.org/10.1038/nsmb.3115	journal	November 2015
Consistent elicitation of cross-clade HIV-neutralizing responses achieved in guinea pigs after fusion peptide priming by repetitive envelope trimer boosting Cheng, Cheng; Xu, Kai; Kong, Rui PLOS ONE, Vol. 14, Issue 4 https://doi.org/10.1371/journal.pone.0215163	journal	April 2019
Immunogenicity of Stabilized HIV-1 Envelope Trimers with Reduced Exposure of Non-neutralizing Epitopes de Taeye, Steven W.; Ozorowski, Gabriel; Torrents de la Peña, Alba Cell, Vol. 163, Issue 7 https://doi.org/10.1016/j.cell.2015.11.056	journal	December 2015
Global site-specific N-glycosylation analysis of HIV envelope glycoprotein Cao, Liwei; Diedrich, Jolene K.; Kulp, Daniel W. Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/ncomms14954	journal	March 2017
Features and development of Coot Emsley, P.; Lohkamp, B.; Scott, W. G. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4 https://doi.org/10.1107/S0907444910007493	journal	March 2010
Building and rebuilding N-glycans in protein structure models van Beusekom, Bart; Wezel, Natasja; Hekkelman, Maarten L. Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 4 https://doi.org/10.1107/S2059798319003875	journal	April 2019
EMRinger: side chain–directed model and map validation for 3D cryo-electron microscopy Barad, Benjamin A.; Echols, Nathaniel; Wang, Ray Yu-Ruei Nature Methods, Vol. 12, Issue 10 https://doi.org/10.1038/nmeth.3541	journal	August 2015
A Native-Like SOSIP.664 Trimer Based on an HIV-1 Subtype B env Gene Pugach, Pavel; Ozorowski, Gabriel; Cupo, Albert Journal of Virology, Vol. 89, Issue 6 https://doi.org/10.1128/JVI.03473-14	journal	January 2015
VDJServer: A Cloud-Based Analysis Portal and Data Commons for Immune Repertoire Sequences and Rearrangements Christley, Scott; Scarborough, Walter; Salinas, Eddie Frontiers in Immunology, Vol. 9 https://doi.org/10.3389/fimmu.2018.00976	journal	May 2018
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
Sequential Immunization Elicits Broadly Neutralizing Anti-HIV-1 Antibodies in Ig Knockin Mice Escolano, Amelia; Steichen, Jon M.; Dosenovic, Pia Cell, Vol. 166, Issue 6 https://doi.org/10.1016/j.cell.2016.07.030	journal	September 2016
Elicitation of Robust Tier 2 Neutralizing Antibody Responses in Nonhuman Primates by HIV Envelope Trimer Immunization Using Optimized Approaches Pauthner, Matthias; Havenar-Daughton, Colin; Sok, Devin Immunity, Vol. 46, Issue 6 https://doi.org/10.1016/j.immuni.2017.05.007	journal	June 2017
Immunoglobulin VH gene diversity and somatic hypermutation during SIV infection of rhesus macaques Guo, Kejun; Halemano, Kalani; Schmitt, Kimberly Immunogenetics, Vol. 67, Issue 7 https://doi.org/10.1007/s00251-015-0844-3	journal	May 2015
Fusion peptide of HIV-1 as a site of vulnerability to neutralizing antibody Kong, R.; Xu, K.; Zhou, T. Science, Vol. 352, Issue 6287 https://doi.org/10.1126/science.aae0474	journal	May 2016
Particulate Array of Well-Ordered HIV Clade C Env Trimers Elicits Neutralizing Antibodies that Display a Unique V2 Cap Approach Martinez-Murillo, Paola; Tran, Karen; Guenaga, Javier Immunity, Vol. 46, Issue 5 https://doi.org/10.1016/j.immuni.2017.04.021	journal	May 2017
Refinement of Protein Structures into Low-Resolution Density Maps Using Rosetta DiMaio, Frank; Tyka, Michael D.; Baker, Matthew L. Journal of Molecular Biology, Vol. 392, Issue 1 https://doi.org/10.1016/j.jmb.2009.07.008	journal	September 2009
Enhancing and shaping the immunogenicity of native-like HIV-1 envelope trimers with a two-component protein nanoparticle Brouwer, Philip J. M.; Antanasijevic, Aleksandar; Berndsen, Zachary Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-12080-1	journal	September 2019
High-quality full-length immunoglobulin profiling with unique molecular barcoding Turchaninova, M. A.; Davydov, A.; Britanova, O. V. Nature Protocols, Vol. 11, Issue 9 https://doi.org/10.1038/nprot.2016.093	journal	August 2016
Comprehensive Antigenic Map of a Cleaved Soluble HIV-1 Envelope Trimer Derking, Ronald; Ozorowski, Gabriel; Sliepen, Kwinten PLOS Pathogens, Vol. 11, Issue 3 https://doi.org/10.1371/journal.ppat.1004767	journal	March 2015
Protein structure homology modeling using SWISS-MODEL workspace Bordoli, Lorenza; Kiefer, Florian; Arnold, Konstantin Nature Protocols, Vol. 4, Issue 1 https://doi.org/10.1038/nprot.2008.197	journal	December 2008
SWISS-MODEL: modelling protein tertiary and quaternary structure using evolutionary information Biasini, Marco; Bienert, Stefan; Waterhouse, Andrew Nucleic Acids Research, Vol. 42, Issue W1 https://doi.org/10.1093/nar/gku340	journal	April 2014
A Broadly Neutralizing Antibody Targets the Dynamic HIV Envelope Trimer Apex via a Long, Rigidified, and Anionic β-Hairpin Structure Lee, Jeong Hyun; Andrabi, Raiees; Su, Ching-Yao Immunity, Vol. 46, Issue 4 https://doi.org/10.1016/j.immuni.2017.03.017	journal	April 2017
NCBI BLAST: a better web interface Johnson, M.; Zaretskaya, I.; Raytselis, Y. Nucleic Acids Research, Vol. 36, Issue Web Server https://doi.org/10.1093/nar/gkn201	journal	May 2008
Stock-based detection of protein oligomeric states in jsPISA Krissinel, Eugene Nucleic Acids Research, Vol. 43, Issue W1 https://doi.org/10.1093/nar/gkv314	journal	April 2015
HIV Vaccine Design to Target Germline Precursors of Glycan-Dependent Broadly Neutralizing Antibodies Steichen, Jon M.; Kulp, Daniel W.; Tokatlian, Talar Immunity, Vol. 45, Issue 3 https://doi.org/10.1016/j.immuni.2016.08.016	journal	September 2016
cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination Punjani, Ali; Rubinstein, John L.; Fleet, David J. Nature Methods, Vol. 14, Issue 3 https://doi.org/10.1038/nmeth.4169	journal	February 2017
Development of broadly neutralizing antibodies in HIV-1 infected elite neutralizers Landais, Elise; Moore, Penny L. Retrovirology, Vol. 15, Issue 1 https://doi.org/10.1186/s12977-018-0443-0	journal	September 2018
IgBLAST: an immunoglobulin variable domain sequence analysis tool Ye, Jian; Ma, Ning; Madden, Thomas L. Nucleic Acids Research, Vol. 41, Issue W1 https://doi.org/10.1093/nar/gkt382	journal	May 2013
[20] Processing of X-ray diffraction data collected in oscillation mode Otwinowski, Zbyszek; Minor, Wladek Macromolecular Crystallography Part A, 307-326 https://doi.org/10.1016/S0076-6879(97)76066-X	book	January 1997
Phaser crystallographic software McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D. Journal of Applied Crystallography, Vol. 40, Issue 4 https://doi.org/10.1107/S0021889807021206	journal	July 2007
Locking the Elbow: Improved Antibody Fab Fragments as Chaperones for Structure Determination Bailey, Lucas J.; Sheehy, Kimberly M.; Dominik, Pawel K. Journal of Molecular Biology, Vol. 430, Issue 3 https://doi.org/10.1016/j.jmb.2017.12.012	journal	February 2018

Similar Records

Improving the Immunogenicity of Native-like HIV-1 Envelope Trimers by Hyperstabilization

Journal Article · Tue Aug 22 00:00:00 EDT 2017 · Cell Reports · OSTI ID:1903966

Torrents de la Peña, Alba; Julien, Jean-Philippe; de Taeye, Steven W.; +26 more

Structure and immunogenicity of a stabilized HIV-1 envelope trimer based on a group-M consensus sequence

Journal Article · Wed May 29 00:00:00 EDT 2019 · Nature Communications · OSTI ID:1903966

Sliepen, Kwinten; Han, Byung Woo; Bontjer, Ilja; +32 more

Electron-Microscopy-Based Epitope Mapping Defines Specificities of Polyclonal Antibodies Elicited during HIV-1 BG505 Envelope Trimer Immunization

Journal Article · Tue Aug 07 00:00:00 EDT 2018 · Immunity · OSTI ID:1903966

Bianchi, Matteo; Turner, Hannah L.; Nogal, Bartek; +10 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
antibodies
antigens
electron microscopy
crystal structure
HIV-1
sequence databases
antibody response
HIV vaccines

Title: Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates

Citation Formats

References (73)

Similar Records

Related Subjects