Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates

Cottrell, Christopher A.; van Schooten, Jelle; Bowman, Charles A.; Yuan, Meng; Oyen, David; Shin, Mia; Morpurgo, Robert; van der Woude, Patricia; van Breemen, Mariëlle; Torres, Jonathan L.; Patel, Raj; Gross, Justin; Sewall, Leigh M.; Copps, Jeffrey; Ozorowski, Gabriel; Nogal, Bartek; Sok, Devin; Rakasz, Eva G.; Labranche, Celia; Vigdorovich, Vladimir; Christley, Scott; Carnathan, Diane G.; Sather, D. Noah; Montefiori, David; Silvestri, Guido; Burton, Dennis R.; Moore, John P.; Wilson, Ian A.; Sanders, Rogier W.; Ward, Andrew B.; van Gils, Marit J.

doi:10.1371/journal.ppat.1008753

Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates

Journal Article · Mon Aug 31 00:00:00 EDT 2020 · PLoS Pathogens

DOI:https://doi.org/10.1371/journal.ppat.1008753· OSTI ID:1903966

^[1]; ^[2]; ^[3]; Yuan, Meng ^[3]; Oyen, David ^[3]; ^[3]; ^[2]; van der Woude, Patricia ^[2]; van Breemen, Mariëlle ^[2]; ^[3]; Patel, Raj ^[3]; ^[3]; ^[3]; ^[3]; ^[3]; ^[3]; ^[3]; Rakasz, Eva G. ^[4]; ^[5]; ^[6] more »

The Scripps Research Institute, La Jolla, CA (United States); OSTI
University of Amsterdam (Netherlands)
The Scripps Research Institute, La Jolla, CA (United States)
University of Wisconsin, Madison, WI (United States)
Duke University, Durham, NC (United States). Medical Center
Seattle Children’s Research Institute, Seattle, WA (United States)
University of Texas Southwestern Medical Center, Dallas, TX (United States)
Scripps Research Institute, San Diego, CA (United States); Emory University, Atlanta, GA (United States)
Seattle Children’s Research Institute, Seattle, Washington, United States
The Scripps Research Institute, La Jolla, CA (United States); Emory University, Atlanta, GA (United States)
Massachusetts Institute of Technology (MIT), Cambridge, MA (United States); Harvard University, Cambridge, MA (United States)
University of Amsterdam (Netherlands); Cornell University, Ithaca, NY (United States)

The induction of broad and potent immunity by vaccines is the key focus of research efforts aimed at protecting against HIV-1 infection. Soluble native-like HIV-1 envelope glycoproteins have shown promise as vaccine candidates as they can induce potent autologous neutralizing responses in rabbits and non-human primates. In this study, monoclonal antibodies were isolated and characterized from rhesus macaques immunized with the BG505 SOSIP.664 trimer to better understand vaccine-induced antibody responses. Our studies reveal a diverse landscape of antibodies recognizing immunodominant strain-specific epitopes and non-neutralizing neo-epitopes. Additionally, we isolated a subset of mAbs against an epitope cluster at the gp120-gp41 interface that recognize the highly conserved fusion peptide and the glycan at position 88 and have characteristics akin to several human-derived broadly neutralizing antibodies.

View Accepted Manuscript (DOE)

Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL); The Scripps Research Institute, La Jolla, CA (United States)

Sponsoring Organization:: Bill and Melinda Gates Foundation; College Scientists Foundation; European Union; National Institutes of Health (NIH); Netherlands Organization for Scientific Research (NWO); Ruth L. Kirschstein Predoctoral Award; USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1903966

Journal Information:: PLoS Pathogens, Journal Name: PLoS Pathogens Journal Issue: 8 Vol. 16; ISSN 1553-7374

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates

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