Male–female communication triggers calcium signatures during fertilization in Arabidopsis

Denninger, Philipp; Bleckmann, Andrea; Lausser, Andreas; Vogler, Frank; Ott, Thomas; Ehrhardt, David W.; Frommer, Wolf B.; Sprunck, Stefanie; Dresselhaus, Thomas; Grossmann, Guido

doi:10.1038/ncomms5645

Male–female communication triggers calcium signatures during fertilization in Arabidopsis

Journal Article · Fri Aug 22 00:00:00 EDT 2014 · Nature Communications

DOI:https://doi.org/10.1038/ncomms5645· OSTI ID:1623955

Denninger, Philipp ^[1]; Bleckmann, Andrea ^[2]; Lausser, Andreas ^[2]; Vogler, Frank ^[2]; Ott, Thomas ^[3]; Ehrhardt, David W. ^[4]; Frommer, Wolf B. ^[4]; Sprunck, Stefanie ^[2]; Dresselhaus, Thomas ^[2]; Grossmann, Guido ^[5]

Univ. Regensburg (Germany). Biochemie-Zentrum Regensburg. Cell Biology and Plant Biology; Univ. Heidelberg (Germany). Centre for Organismal Studies (COS). Cell Networks-Cluster of Excellence; DOE/OSTI
Univ. Regensburg (Germany). Biochemie-Zentrum Regensburg. Cell Biology and Plant Biology
Ludwig Maximilians Univ. Munchen (Germany). Inst. of Genetics
Carnegie Inst. of Science, Stanford, CA (United States). Dept. of Plant Biology
Univ. Heidelberg (Germany). Centre for Organismal Studies (COS). Cell Networks-Cluster of Excellence; Carnegie Inst. of Science, Stanford, CA (United States). Dept. of Plant Biology

Cell–cell communication and interaction is critical during fertilization and triggers free cytosolic calcium ([Ca2 þ ]cyto) as a key signal for egg activation and a polyspermy block in animal oocytes. Fertilization in flowering plants is more complex, involving interaction of a pollen tube with egg adjoining synergid cells, culminating in release of two sperm cells and their fusion with the egg and central cell, respectively. Here, we report the occurrence and role of [Ca2 þ ]cyto signals during the entire double fertilization process in Arabidopsis. [Ca2 þ ]cyto oscillations are initiated in synergid cells after physical contact with the pollen tube apex. In egg and central cells, a short [Ca2 þ ]cyto transient is associated with pollen tube burst and sperm cell arrival. A second extended [Ca2 þ ]cyto transient solely in the egg cell is correlated with successful fertilization. Thus, each female cell type involved in double fertilization displays a characteristic [Ca2 þ ]cyto signature differing by timing and behaviour from [Ca2 þ ]cyto waves reported in mammals.

View Accepted Manuscript (DOE)

Research Organization:: Carnegie Inst. of Washington, Washington, DC (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: FG02-04ER15542

OSTI ID:: 1623955

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 5; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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