Hybrid SSF/SHF Processing of SO2 Pretreated Wheat Straw—Tuning Co-fermentation by Yeast Inoculum Size and Hydrolysis Time

Cassells, B.; Karhumaa, K.; Sànchez i Nogué, V.; Lidén, Gunnar

doi:10.1007/s12010-016-2229-y

Hybrid SSF/SHF Processing of SO₂ Pretreated Wheat Straw—Tuning Co-fermentation by Yeast Inoculum Size and Hydrolysis Time

Journal Article · Thu Sep 08 04:00:00 EDT 2016 · Applied Biochemistry and Biotechnology

DOI:https://doi.org/10.1007/s12010-016-2229-y· OSTI ID:1623627

Cassells, B. ^[1]; Karhumaa, K. ^[2]; Sànchez i Nogué, V. ^[3]; Lidén, Gunnar ^[4]

Lund Univ. (Sweden); Novozymes A/S, Bagsvaerd (Denmark); DOE/OSTI
C5 Ligno Technologies in Lund AB (Sweden); Hansa Medical AB, Lund (Sweden)
C5 Ligno Technologies in Lund AB (Sweden); National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Lund Univ. (Sweden)

Wheat straw is one of the main agricultural residues of interest for bioethanol production. This work examines conversion of steam-pretreated wheat straw (using SO₂ as a catalyst) in a hybrid process consisting of a short enzymatic prehydrolysis step and a subsequent simultaneous saccharification and fermentation (SSF) step with a xylose-fermenting strain of Saccharomyces cerevisiae. A successful process requires a balanced design of reaction time and temperature in the prehydrolysis step and yeast inoculum size and temperature in the SSF step. The pretreated material obtained after steam pretreatment at 210 °C for 5 min using 2.5 % SO₂ (based on moisture content) showed a very good enzymatic digestibility at 45 °C but clearly lower at 30 °C. Furthermore, the pretreatment liquid was found to be rather inhibitory to the yeast, partly due to a furfural content of more than 3 g/L. The effect of varying the yeast inoculum size in this medium was assessed, and at a yeast inoculum size of 4 g/L, a complete conversion of glucose and a 90 % conversion of xylose were obtained within 50 h. An ethanol yield (based on the glucan and xylan in the pretreated material) of 0.39 g/g was achieved for a process with this yeast inoculum size in a hybrid process (10 % water-insoluble solid (WIS)) with 4 h prehydrolysis time and a total process time of 96 h. The obtained xylose conversion was 95 %. A longer prehydrolysis time or a lower yeast inoculum size resulted in incomplete xylose conversion.

View Accepted Manuscript (DOE)

Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC); Swedish Energy Agency

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1623627

Journal Information:: Applied Biochemistry and Biotechnology, Journal Name: Applied Biochemistry and Biotechnology Journal Issue: 2 Vol. 181; ISSN 0273-2289

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Hybrid SSF/SHF Processing of SO2 Pretreated Wheat Straw—Tuning Co-fermentation by Yeast Inoculum Size and Hydrolysis Time

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Hybrid SSF/SHF Processing of SO₂ Pretreated Wheat Straw—Tuning Co-fermentation by Yeast Inoculum Size and Hydrolysis Time