Towards sustainable bioplastic production using the photoautotrophic bacterium Rhodopseudomonas palustris TIE-1

Ranaivoarisoa, Tahina Onina; Singh, Rajesh; Rengasamy, Karthikeyan; Guzman, Michael S.; Bose, Arpita

doi:10.1007/s10295-019-02165-7

Title: Towards sustainable bioplastic production using the photoautotrophic bacterium Rhodopseudomonas palustris TIE-1

Journal Article · Tue Oct 01 00:00:00 EDT 2019 · Journal of Industrial Microbiology and Biotechnology

DOI:https://doi.org/10.1007/s10295-019-02165-7· OSTI ID:1619392

Ranaivoarisoa, Tahina Onina ^[1]; Singh, Rajesh ^[1]; Rengasamy, Karthikeyan ^[1]; Guzman, Michael S. ^[1]; Bose, Arpita ^[1]

grid.4367.6 0000 0001 2355 7002 Department of Biology Washington University in St. Louis One Brookings Drive 63130 St. Louis MO USA

Abstract Bacterial synthesis of polyhydroxybutyrates (PHBs) is a potential approach for producing biodegradable plastics. This study assessed the ability of Rhodopseudomonas palustris TIE-1 to produce PHBs under various conditions. We focused on photoautotrophy using a poised electrode (photoelectroautotrophy) or ferrous iron (photoferroautotrophy) as electron donors. Growth conditions were tested with either ammonium chloride or dinitrogen gas as the nitrogen source. Although TIE-1’s capacity to produce PHBs varied fairly under different conditions, photoelectroautotrophy and photoferroautotrophy showed the highest PHB electron yield and the highest specific PHB productivity, respectively. Gene expression analysis showed that there was no differential expression in PHB biosynthesis genes. This suggests that the variations in PHB accumulation might be post-transcriptionally regulated. This is the first study to systematically quantify the amount of PHB produced by a microbe via photoelectroautotrophy and photoferroautotrophy. This work could lead to sustainable bioproduction using abundant resources such as light, electricity, iron, and carbon dioxide.

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Research Organization:: Washington Univ., St. Louis, MO (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: DESC0014613; SC0014613

OSTI ID:: 1619392

Alternate ID(s):: OSTI ID: 1623614

Journal Information:: Journal of Industrial Microbiology and Biotechnology, Journal Name: Journal of Industrial Microbiology and Biotechnology Vol. 46 Journal Issue: 9-10; ISSN 1367-5435

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: Germany

Language:: English

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Cited by: 42 works

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