An insoluble iron complex coated cathode enhances direct electron uptake by Rhodopseudomonas palustris TIE-1

Rengasamy, Karthikeyan; Ranaivoarisoa, Tahina; Singh, Rajesh; Bose, Arpita

doi:10.1016/j.bioelechem.2018.03.015

An insoluble iron complex coated cathode enhances direct electron uptake by Rhodopseudomonas palustris TIE-1

Journal Article · Thu Apr 05 04:00:00 EDT 2018 · Bioelectrochemistry

DOI:https://doi.org/10.1016/j.bioelechem.2018.03.015· OSTI ID:1538028

Rengasamy, Karthikeyan ^[1]; Ranaivoarisoa, Tahina ^[2]; Singh, Rajesh ^[2]; Bose, Arpita ^[2]

Washington University, St. Louis, MO (United States); DOE/OSTI
Washington University, St. Louis, MO (United States)

Microbial electrosynthesis (MES) is a promising bioelectrochemical approach to produce biochemicals. A previous study showed that Rhodopseudomonas palustris TIE-1 can directly use poised electrodes as electron donors for photoautotrophic growth at cathodic potentials that avoid electrolytic H2 production (photoelectroautotrophy). To make TIE-1 an effective biocatalyst for MES, we need to improve its electron uptake ability and growth under photoelectroautotrophic conditions. Because TIE-1 interacts with various forms of iron while using it as a source of electrons for photoautotrophy (photoferroautotrophy), here we tested the ability of iron-based redox mediators to enhance direct electron uptake. Our data show that soluble iron cannot act as a redox mediator for electron uptake by TIE-1 from a cathode poised at +100 mV vs. Standard Hydrogen electrode. We then tested whether an immobilized iron-based redox mediator Prussian blue (PB) can enhance electron uptake by TIE-1. Chronoamperometry indicates that cathodic current uptake by TIE-1 increased from 1.47 ± 0.04 to 5.6 ± 0.09 μA/cm² (3.8 times). Overall, our data show that immobilized PB can enhance direct electron uptake by TIE-1.

View Accepted Manuscript (DOE)

Research Organization:: Washington University, St. Louis, MO (United States)

Sponsoring Organization:: USDOE Office of Science (SC); David and Lucile Packard Foundation

Grant/Contract Number:: SC0014613

OSTI ID:: 1538028

Alternate ID(s):: OSTI ID: 1548467

Journal Information:: Bioelectrochemistry, Journal Name: Bioelectrochemistry Journal Issue: C Vol. 122; ISSN 1567-5394

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Prussian blue
Rhodopseudomonas palustris
TIE-1
electron uptake
microbial electrosynthesis
photoelectroautotrophy

An insoluble iron complex coated cathode enhances direct electron uptake by Rhodopseudomonas palustris TIE-1

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