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Title: Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria

Journal Article · · Microorganisms
 [1];  [2];  [3];  [4];  [4];  [2];  [5];  [2]
  1. Univ. of Washington, Seattle, WA (United States); Univ. of California, Irvine, CA (United States)
  2. Univ. of Washington, Seattle, WA (United States)
  3. Univ. of Washington, Seattle, WA (United States); Denali Advanced Integration, Redmond, WA (United States)
  4. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  5. Univ. of Washington, Seattle, WA (United States); San Diego State Univ., CA (United States)
+ Show Author Affiliations

Gram-positive methylotrophic bacteria have been known for a long period of time, some serving as model organisms for characterizing the specific details of methylotrophy pathways/enzymes within this group. However, genome-based knowledge of methylotrophy within this group has been so far limited to a single species, Bacillus methanolicus (Firmicutes). The paucity of whole-genome data for Gram-positive methylotrophs limits our global understanding of methylotrophy within this group, including their roles in specific biogeochemical cycles, as well as their biotechnological potential. Here, we describe the isolation of seven novel strains of Gram-positive methylotrophs that include two strains of Bacillus and five representatives of Actinobacteria classified within two genera, Arthrobacter and Mycobacterium. We report whole-genome sequences for these isolates and present comparative analysis of the methylotrophy functional modules within these genomes. The genomic sequences of these seven novel organisms, all capable of growth on methylated amines, present an important reference dataset for understanding the genomic basis of methylotrophy in Gram-positive methylotrophic bacteria. This study is a major contribution to the field of methylotrophy, aimed at closing the gap in the genomic knowledge of methylotrophy within this diverse group of bacteria.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF)
Grant/Contract Number:
AC02-05CH11231; MCB-0950183; SC0010556
OSTI ID:
1619069
Journal Information:
Microorganisms, Vol. 3, Issue 1; ISSN 2076-2607
Publisher:
MDPICopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (4)

Methylamine Activates Glucose Uptake in Human Adipocytes Without Overpassing Action of Insulin or Stimulating its Secretion in Pancreatic Islets journal August 2019
Natural Selection in Synthetic Communities Highlights the Roles of Methylococcaceae and Methylophilaceae and Suggests Differential Roles for Alternative Methanol Dehydrogenases in Methane Consumption journal December 2017
Methylotrophs in natural habitats: current insights through metagenomics journal June 2015
A Synthetic Ecology Perspective: How Well Does Behavior of Model Organisms in the Laboratory Predict Microbial Activities in Natural Habitats? journal June 2016

Figures / Tables (6)

Table 1(p. 5)table Table 1
Figure 1(p. 7)figure Figure 1
Table 2 : Part 1(p. 9)table Table 2 : Part 1
Table 2 : Part 2(p. 10)table Table 2 : Part 2
Table 2 : Part 3(p. 11)table Table 2 : Part 3
Figure 2(p. 12)figure Figure 2

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
methylotrophy
genomics
Arthrobacter
Bacillus
Mycobacterium
Lake Washington