Multiphyletic origins of methylotrophy in A lphaproteobacteria , exemplified by comparative genomics of L ake W ashington isolates
- Department of Chemical Engineering University of Washington Seattle WA USA, eScience Institute University of Washington Seattle WA USA
- Department of Chemical Engineering University of Washington Seattle WA USA
- Department of Chemical Engineering University of Washington Seattle WA USA, International Community Health Services Seattle WA USA
- Department of Chemical Engineering University of Washington Seattle WA USA, University of Georgia Athens GA USA
- Los Alamos National Laboratory Los Alamos NM USA
- DOE Joint Genome Institute Walnut Creek CA USA
- Department of Microbiology University of Washington Seattle WA USA, Department of Biology San Diego State University San Diego CA USA
- Department of Chemical Engineering University of Washington Seattle WA USA, Department of Microbiology University of Washington Seattle WA USA
Summary
We sequenced the genomes of 19 methylotrophic isolates from L ake W ashington, which belong to nine genera within eight families of the A lphaproteobacteria , two of the families being the newly proposed families. Comparative genomic analysis with a focus on methylotrophy metabolism classifies these strains into heterotrophic and obligately or facultatively autotrophic methylotrophs. The most persistent metabolic modules enabling methylotrophy within this group are the N ‐methylglutamate pathway, the two types of methanol dehydrogenase ( MxaFI and XoxF ), the tetrahydromethanopterin pathway for formaldehyde oxidation, the serine cycle and the ethylmalonyl‐ CoA pathway. At the same time, a great potential for metabolic flexibility within this group is uncovered, with different combinations of these modules present. Phylogenetic analysis of key methylotrophy functions reveals that the serine cycle must have evolved independently in at least four lineages of A lphaproteobacteria and that all methylotrophy modules seem to be prone to lateral transfers as well as deletions.
- Sponsoring Organization:
- USDOE
- Grant/Contract Number:
- NONE; SC0010556; AC02-05CH11231
- OSTI ID:
- 1401604
- Journal Information:
- Environmental Microbiology, Journal Name: Environmental Microbiology Journal Issue: 3 Vol. 17; ISSN 1462-2912
- Publisher:
- Wiley-BlackwellCopyright Statement
- Country of Publication:
- United Kingdom
- Language:
- English
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