In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions

Attia, Mohamed A.; Nelson, Cassandra E.; Offen, Wendy A.; Jain, Namrata; Davies, Gideon J.; Gardner, Jeffrey G.; Brumer, Harry

doi:10.1186/s13068-018-1039-6

Title: In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions

Journal Article · Sat Feb 17 00:00:00 EST 2018 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-018-1039-6· OSTI ID:1618718

Attia, Mohamed A.; Nelson, Cassandra E.; Offen, Wendy A.; Jain, Namrata; Davies, Gideon J.; Gardner, Jeffrey G.; Brumer, Harry

Xyloglucan (XyG) is a ubiquitous and fundamental polysaccharide of plant cell walls. Due to its structural complexity, XyG requires a combination of backbone-cleaving and sidechain-debranching enzymes for complete deconstruction into its component monosaccharides. The soil saprophyte Cellvibrio japonicus has emerged as a genetically tractable model system to study biomass saccharification, in part due to its innate capacity to utilize a wide range of plant polysaccharides for growth. Whereas the downstream debranching enzymes of the xyloglucan utilization system of C. japonicus have been functionally characterized, the requisite backbone-cleaving endo-xyloglucanases were unresolved.

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Research Organization:: Univ. of Maryland, Baltimore, MD (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0014183

OSTI ID:: 1618718

Alternate ID(s):: OSTI ID: 1504757

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Vol. 11 Journal Issue: 1; ISSN 1754-6834

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: Netherlands

Language:: English

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Cited by: 22 works

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Xyloglucan
Saccharification
Glycoside hydrolase
Cellvibrio japonicus
Saprophyte

MOESM1 of In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions Attia, Mohamed; Nelson, Cassandra; Offen, Wendy https://doi.org/10.6084/m9.figshare.5899978 The current record is supplemented by this text	text	January 2018
In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions Attia, Mohamed; Nelson, Cassandra; Offen, Wendy https://doi.org/10.6084/m9.figshare.c.4008514.v1 The current record is supplemented by this collection	collection	January 2018
MOESM1 of In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions Attia, Mohamed; Nelson, Cassandra; Offen, Wendy https://doi.org/10.6084/m9.figshare.5899978.v1 The current record is supplemented by this text	text	January 2018