Controlling microbial contamination during hydrolysis of AFEX-pretreated corn stover and switchgrass: effects on hydrolysate composition, microbial response and fermentation

Serate, Jose; Xie, Dan; Pohlmann, Edward; Donald, Jr., Charles; Shabani, Mahboubeh; Hinchman, Li; Higbee, Alan; Mcgee, Mick; La Reau, Alex; Klinger, Grace E.; Li, Sheena; Myers, Chad L.; Boone, Charles; Bates, Donna M.; Cavalier, Dave; Eilert, Dustin; Oates, Lawrence G.; Sanford, Gregg; Sato, Trey K.; Dale, Bruce; Landick, Robert; Piotrowski, Jeff; Ong, Rebecca Garlock; Zhang, Yaoping

doi:10.1186/s13068-015-0356-2

Title: Controlling microbial contamination during hydrolysis of AFEX-pretreated corn stover and switchgrass: effects on hydrolysate composition, microbial response and fermentation

Journal Article · Sat Nov 14 00:00:00 EST 2015 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-015-0356-2· OSTI ID:1618622

Serate, Jose; Xie, Dan; Pohlmann, Edward; Donald, Jr., Charles; Shabani, Mahboubeh; Hinchman, Li; Higbee, Alan; Mcgee, Mick; La Reau, Alex; Klinger, Grace E.; Li, Sheena; Myers, Chad L.; Boone, Charles; Bates, Donna M.; Cavalier, Dave; Eilert, Dustin; Oates, Lawrence G.; Sanford, Gregg; Sato, Trey K.; Dale, Bruce more »

Microbial conversion of lignocellulosic feedstocks into biofuels remains an attractive means to produce sustainable energy. It is essential to produce lignocellulosic hydrolysates in a consistent manner in order to study microbial performance in different feedstock hydrolysates. Because of the potential to introduce microbial contamination from the untreated biomass or at various points during the process, it can be difficult to control sterility during hydrolysate production. In this study, we compared hydrolysates produced from AFEX-pretreated corn stover and switchgrass using two different methods to control contamination: either by autoclaving the pretreated feedstocks prior to enzymatic hydrolysis, or by introducing antibiotics during the hydrolysis of non-autoclaved feedstocks. We then performed extensive chemical analysis, chemical genomics, and comparative fermentations to evaluate any differences between these two different methods used for producing corn stover and switchgrass hydrolysates. Autoclaving the pretreated feedstocks could eliminate the contamination for a variety of feedstocks, whereas the antibiotic gentamicin was unable to control contamination consistently during hydrolysis. Compared to the addition of gentamicin, autoclaving of biomass before hydrolysis had a minimal effect on mineral concentrations, and showed no significant effect on the two major sugars (glucose and xylose) found in these hydrolysates. However, autoclaving elevated the concentration of some furanic and phenolic compounds. Chemical genomics analyses using Saccharomyces cerevisiae strains indicated a high correlation between the AFEX-pretreated hydrolysates produced using these two methods within the same feedstock, indicating minimal differences between the autoclaving and antibiotic methods. Comparative fermentations with S. cerevisiae and Zymomonas mobilis also showed that autoclaving the AFEX-pretreated feedstocks had no significant effects on microbial performance in these hydrolysates. In conclusion, our results showed that autoclaving the pretreated feedstocks offered advantages over the addition of antibiotics for hydrolysate production. The autoclaving method produced a more consistent quality of hydrolysate.

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Research Organization:: Wisconsin Alumni Research Foundation, Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: FC02-07ER64494

OSTI ID:: 1618622

Alternate ID(s):: OSTI ID: 1241145

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Vol. 8 Journal Issue: 1; ISSN 1754-6834

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: Netherlands

Language:: English

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Cited by: 32 works

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09 BIOMASS FUELS
biomass feedstock
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chemical genomics
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Controlling microbial contamination during hydrolysis of AFEX-pretreated corn stover and switchgrass: effects on hydrolysate composition, microbial response and fermentation Serate, Jose; Xie, Dan; Pohlmann, Edward https://doi.org/10.6084/m9.figshare.c.3625271 The current record is supplemented by this dataset	dataset	November 2015
MOESM3 of Controlling microbial contamination during hydrolysis of AFEX-pretreated corn stover and switchgrass: effects on hydrolysate composition, microbial response and fermentation Serate, Jose; Xie, Dan; Pohlmann, Edward https://doi.org/10.6084/m9.figshare.c.3625271_d1.v1 The current record is supplemented by this figure	figure	November 2015
MOESM5 of Controlling microbial contamination during hydrolysis of AFEX-pretreated corn stover and switchgrass: effects on hydrolysate composition, microbial response and fermentation Serate, Jose; Xie, Dan; Pohlmann, Edward https://doi.org/10.6084/m9.figshare.c.3625271_d2 The current record is supplemented by this text	text	January 2015
MOESM5 of Controlling microbial contamination during hydrolysis of AFEX-pretreated corn stover and switchgrass: effects on hydrolysate composition, microbial response and fermentation Serate, Jose; Xie, Dan; Pohlmann, Edward https://doi.org/10.6084/m9.figshare.c.3625271_d2.v1 The current record is supplemented by this dataset	dataset	November 2015
MOESM2 of Controlling microbial contamination during hydrolysis of AFEX-pretreated corn stover and switchgrass: effects on hydrolysate composition, microbial response and fermentation Serate, Jose; Xie, Dan; Pohlmann, Edward https://doi.org/10.6084/m9.figshare.c.3625271_d3 The current record is supplemented by this dataset	dataset	January 2015
MOESM2 of Controlling microbial contamination during hydrolysis of AFEX-pretreated corn stover and switchgrass: effects on hydrolysate composition, microbial response and fermentation Serate, Jose; Xie, Dan; Pohlmann, Edward https://doi.org/10.6084/m9.figshare.c.3625271_d3.v1 The current record is supplemented by this dataset	dataset	November 2015
MOESM4 of Controlling microbial contamination during hydrolysis of AFEX-pretreated corn stover and switchgrass: effects on hydrolysate composition, microbial response and fermentation Serate, Jose; Xie, Dan; Pohlmann, Edward https://doi.org/10.6084/m9.figshare.c.3625271_d4 The current record is supplemented by this audiovisual	audiovisual	January 2015
MOESM4 of Controlling microbial contamination during hydrolysis of AFEX-pretreated corn stover and switchgrass: effects on hydrolysate composition, microbial response and fermentation Serate, Jose; Xie, Dan; Pohlmann, Edward https://doi.org/10.6084/m9.figshare.c.3625271_d4.v1 The current record is supplemented by this figure	figure	November 2015
MOESM1 of Controlling microbial contamination during hydrolysis of AFEX-pretreated corn stover and switchgrass: effects on hydrolysate composition, microbial response and fermentation Serate, Jose; Xie, Dan; Pohlmann, Edward https://doi.org/10.6084/M9.FIGSHARE.C.3625271_D5 The current record is supplemented by this audiovisual	audiovisual	January 2015
MOESM1 of Controlling microbial contamination during hydrolysis of AFEX-pretreated corn stover and switchgrass: effects on hydrolysate composition, microbial response and fermentation Serate, Jose; Xie, Dan; Pohlmann, Edward https://doi.org/10.6084/m9.figshare.c.3625271_d5.v1 The current record is supplemented by this dataset	dataset	November 2015
Controlling microbial contamination during hydrolysis of AFEX-pretreated corn stover and switchgrass: effects on hydrolysate composition, microbial response and fermentation [Supplemental Data] Serate, Jose; Xie, Dan; Pohlmann, Edward https://doi.org/10.6084/m9.figshare.c.3625271.v1 The current record is supplemented by this collection	collection	November 2015