Decoupling copolymer, lipid and carbon nanotube interactions in hybrid, biomimetic vesicles

Hammons, Joshua A.; Ingólfsson, Helgi I.; Lee, Jonathan R.  I.; Carpenter, Tim S.; Sanborn, Jeremy; Tunuguntla, Ramya; Yao, Yun-Chiao; Weiss, Thomas M.; Noy, Aleksandr; Van Buuren, Tony

doi:10.1039/c9nr09973e

Decoupling copolymer, lipid and carbon nanotube interactions in hybrid, biomimetic vesicles

Journal Article · Thu Mar 05 00:00:00 EST 2020 · Nanoscale

DOI:https://doi.org/10.1039/c9nr09973e· OSTI ID:1616992

^[1]; Ingólfsson, Helgi I. ^[1]; Lee, Jonathan R. I. ^[1]; Carpenter, Tim S. ^[1]; Sanborn, Jeremy ^[1]; Tunuguntla, Ramya ^[1]; Yao, Yun-Chiao ^[1]; Weiss, Thomas M. ^[1]; Noy, Aleksandr ^[1]; Van Buuren, Tony ^[1]

Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

Bilayer vesicles that mimic a real biological cell can be tailored to carry out a specific function by manipulating the molecular composition of the amphiphiles. These bio-inspired and bio-mimetic structures are increasingly being employed for a number of applications from drug delivery to water purification and beyond. Complex hybrid bilayers are the key building blocks for fully synthetic vesicles that can mimic biological cell membranes, which often contain a wide variety of molecular species. While the assembly and morpholgy of pure phospholid bilayer vesicles is well understood, the functionality and structure dramaticlly changes when copolymer and/or carbon nanotube porins (CNTP) are added. The aim of this study is to understand how the collective molecular interactions within hybrid vesicles affect their nanoscale structure and properties. In situ small and wide angle X-ray scattering (SAXS/WAXS) and molecular dynamics simulations (MD) are used to investigate the morphological effect of molecular interactions between polybutadiene polyethylene oxide, lipids and carbon nanotubes (CNT) within the hybrid vesicle bilayer. Within the lipid/copolymer system, the hybrid bilayer morphology transitions from phase separated lipid and compressed copolymer at low copolymer loadings to a mixed bilayer where opposing lipids are mostly separated from the inner region. This transition begins between 60 wt% and 70 wt%, with full homogenization observed by 80 wt% copolymer. The incorporation of CNT into the hybrid vesicles increases the bilayer thickness and enhances the bilayer symmetry. Analysis of the WAXS and MD indicate that the CNT–dioleoyl interactions are much stronger than the CNT–polybutadiene.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States); SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE; USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: AC02-76SF00515; AC52-07NA27344

OSTI ID:: 1616992

Alternate ID(s):: OSTI ID: 1604106
OSTI ID: 1716590

Report Number(s):: LLNL-JRNL--797277; LLNL-JRNL-797277

Journal Information:: Nanoscale, Journal Name: Nanoscale Journal Issue: 11 Vol. 12; ISSN NANOHL; ISSN 2040-3364

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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