Peeling back the layers of crassulacean acid metabolism: functional differentiation between Kalanchoë fedtschenkoi epidermis and mesophyll proteomes

Abraham, Paul E.; Hurtado Castano, Natalia; Cowan‐Turner, Daniel; Barnes, Jeremy; Poudel, Suresh; Hettich, Robert; Flütsch, Sabrina; Santelia, Diana; Borland, Anne M.

doi:10.1111/tpj.14757

Title: Peeling back the layers of crassulacean acid metabolism: functional differentiation between Kalanchoë fedtschenkoi epidermis and mesophyll proteomes

Journal Article · Tue Apr 28 00:00:00 EDT 2020 · The Plant Journal

DOI:https://doi.org/10.1111/tpj.14757· OSTI ID:1616372

Abraham, Paul E. ^[1]; Hurtado Castano, Natalia ^[2]; Cowan‐Turner, Daniel ^[3]; Barnes, Jeremy ^[3]; Poudel, Suresh ^[4]; Hettich, Robert ^[1]; Flütsch, Sabrina ^[5]; Santelia, Diana ^[5];

^[6]

Chemical Sciences Division Oak Ridge National Laboratory Oak Ridge TN 37831 USA
School of Natural and Environmental Sciences Newcastle University Newcastle upon Tyne NE1 7RU UK, Department of Molecular Biology and Biotechnology University of Sheffield Sheffield S10 2TN UK
School of Natural and Environmental Sciences Newcastle University Newcastle upon Tyne NE1 7RU UK
Chemical Sciences Division Oak Ridge National Laboratory Oak Ridge TN 37831 USA, Department of Genome Science and Technology University of Tennessee Knoxville TN 37996 USA
Institute of Integrative Biology ETH Zürich Switzerland
School of Natural and Environmental Sciences Newcastle University Newcastle upon Tyne NE1 7RU UK, Biosciences Division Oak Ridge National Laboratory Oak Ridge TN 37831 USA

Crassulacean acid metabolism (CAM) is a specialized mode of photosynthesis that offers the potential to engineer improved water-use efficiency (WUE) and drought resilience in C₃ plants while sustaining productivity in the hotter and drier climates that are predicted for much of the world. CAM species show an inverted pattern of stomatal opening and closing across the diel cycle, which conserves water and provides a means of maintaining growth in hot, water-limited environments. Recent genome sequencing of the constitutive model CAM species Kalanchoë fedtschenkoi provides a platform for elucidating the ensemble of proteins that link photosynthetic metabolism with stomatal movement, and that protect CAM plants from harsh environmental conditions. We describe a large-scale proteomics analysis to characterize and compare proteins, as well as diel changes in their abundance in guard cell-enriched epidermis and mesophyll cells from leaves of K. fedtschenkoi. Proteins implicated in processes that encompass respiration, the transport of water and CO₂, stomatal regulation, and CAM biochemistry are highlighted and discussed. Diel rescheduling of guard cell starch turnover in K. fedtschenkoi compared with that observed in Arabidopsis is reported and tissue-specific localization in the epidermis and mesophyll of isozymes implicated in starch and malate turnover are discussed in line with the contrasting roles for these metabolites within the CAM mesophyll and stomatal complex. These data reveal the proteins and the biological processes enriched in each layer and provide key information for studies aiming to adapt plants to hot and dry environments by modifying leaf physiology for improved plant sustainability.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); Swiss National Science Foundation (SNSF)

Grant/Contract Number:: AC05-00OR22725; SC0008834; 31003A‐166539/1

OSTI ID:: 1616372

Alternate ID(s):: OSTI ID: 1616373; OSTI ID: 1617821

Journal Information:: The Plant Journal, Journal Name: The Plant Journal; ISSN 0960-7412

Publisher:: Society for Experimental BiologyCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 9 works

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59 BASIC BIOLOGICAL SCIENCES
crassulacean acid metabolism
proteomics
epidermis
guard cell
starch metabolism

Title: Peeling back the layers of crassulacean acid metabolism: functional differentiation between Kalanchoë fedtschenkoi epidermis and mesophyll proteomes

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