Incorporation of Melittin Enhances Interfacial Fluidity of Bicontinuous Microemulsions

Sharma, V. K.; Hayes, D. G.; Gupta, S.; Urban, V. S.; O’Neill, H. M.; Pingali, S. V.; Ohl, M.; Mamontov, E.

doi:10.1021/acs.jpcc.9b00103

Incorporation of Melittin Enhances Interfacial Fluidity of Bicontinuous Microemulsions

Journal Article · Fri Mar 22 00:00:00 EDT 2019 · Journal of Physical Chemistry. C

DOI:https://doi.org/10.1021/acs.jpcc.9b00103· OSTI ID:1615940

^[1]; Hayes, D. G. ^[2]; ^[3]; ^[4]; ^[4]; ^[4]; Ohl, M. ^[5]; ^[4]

Bhabha Atomic Research Centre, Mumbai (India); Louisiana State University
Univ. of Tennessee, Knoxville, TN (United States)
Louisiana State Univ., Baton Rouge, LA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Spallation Neutron Source (SNS) outstation Juelich Centre for Neutron Science (JCNS), Forschungszentrum Juelich GmbH
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Neutron Sciences Directorate
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Spallation Neutron Source (SNS) outstation Juelich Centre for Neutron Science (JCNS), Forschungszentrum Juelich GmbH

The effect of an antimicrobial peptide, melittin, on the long-range collective and local undulation motions of bicontinuous microemulsions (BμEs) was evaluated using dynamic light scattering (DLS) and neutron spin echo (NSE) spectroscopy. Small-angle neutron scattering (SANS) was also used to study the effect of melittin on the structure and the correlation length of BμEs. BμEs were isolated from Winsor-III systems formed by mixing aqueous melittin solutions containing sodium dodecyl sulfate with dodecane/1-pentanol mixtures at optimal salinity. An increase of melittin concentration linearly increased the diffusivity of the microemulsions (D_DLS) and decreased the bending rigidity (κ_NSE) up to an aqueous melittin concentration of 2 g/L, thus indicating an increase of interfacial fluidity. Further increase of melittin concentration above 2 g/L did not change D_DLS and slightly increased κ_NSE. The changes reflect differences of interaction between melittin and BμE surfactant monolayers at different melittin concentrations. At low concentrations, melittin is highly associated with the surfactant monolayers, likely ion-paired to the sulfate surfactant head groups and perhaps partially penetrating into the surfactant tail region. Above 2 g/L, an apparent saturation concentration, added melittin is less strongly associated with the monolayers. In contrast, the bare bending constant derived from SANS data (κ_bare) underwent only a minor decrease. Noting that κ_bare reflects contributions from both the elasticity and saddle-splay moduli, this suggests that κ_bare is not an effective indicator of dynamics for the BμEs investigated. Our study demonstrates that amphiphilic solubilizates can have a complex impact on dynamics of surfactant monolayers in microemulsions, with implications for transmembrane diffusion and the kinetics of release from the microemulsion phase, which are important characteristics for the use of BμEs as delivery vehicles and host systems for (bio)chemical reactions.

View Accepted Manuscript (DOE)

Research Organization:: Louisiana State Univ., Baton Rouge, LA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: SC0012432

OSTI ID:: 1615940

Journal Information:: Journal of Physical Chemistry. C, Journal Name: Journal of Physical Chemistry. C Journal Issue: 17 Vol. 123; ISSN 1932-7447

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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Incorporation of Melittin Enhances Interfacial Fluidity of Bicontinuous Microemulsions

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