Incorporation of Melittin Enhances Interfacial Fluidity of Bicontinuous Microemulsions

Sharma, V. K.; Hayes, D. G.; Gupta, S.; Urban, V. S.; O’Neill, H. M.; Pingali, S. V.; Ohl, M.; Mamontov, E.

doi:10.1021/acs.jpcc.9b00103

Title: Incorporation of Melittin Enhances Interfacial Fluidity of Bicontinuous Microemulsions

Journal Article · Fri Mar 22 00:00:00 EDT 2019 · Journal of Physical Chemistry. C

DOI:https://doi.org/10.1021/acs.jpcc.9b00103· OSTI ID:1615940

^[1]; Hayes, D. G. ^[2];

^[3];

^[4];

^[4]; Ohl, M. ^[5];

^[4]

Bhabha Atomic Research Centre, Mumbai (India)
Univ. of Tennessee, Knoxville, TN (United States)
Louisiana State Univ., Baton Rouge, LA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Spallation Neutron Source (SNS) outstation Juelich Centre for Neutron Science (JCNS), Forschungszentrum Juelich GmbH
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Neutron Sciences Directorate
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Spallation Neutron Source (SNS) outstation Juelich Centre for Neutron Science (JCNS), Forschungszentrum Juelich GmbH

The effect of an antimicrobial peptide, melittin, on the long-range collective and local undulation motions of bicontinuous microemulsions (BμEs) was evaluated using dynamic light scattering (DLS) and neutron spin echo (NSE) spectroscopy. Small-angle neutron scattering (SANS) was also used to study the effect of melittin on the structure and the correlation length of BμEs. BμEs were isolated from Winsor-III systems formed by mixing aqueous melittin solutions containing sodium dodecyl sulfate with dodecane/1-pentanol mixtures at optimal salinity. An increase of melittin concentration linearly increased the diffusivity of the microemulsions (D_DLS) and decreased the bending rigidity (κ_NSE) up to an aqueous melittin concentration of 2 g/L, thus indicating an increase of interfacial fluidity. Further increase of melittin concentration above 2 g/L did not change D_DLS and slightly increased κ_NSE. The changes reflect differences of interaction between melittin and BμE surfactant monolayers at different melittin concentrations. At low concentrations, melittin is highly associated with the surfactant monolayers, likely ion-paired to the sulfate surfactant head groups and perhaps partially penetrating into the surfactant tail region. Above 2 g/L, an apparent saturation concentration, added melittin is less strongly associated with the monolayers. In contrast, the bare bending constant derived from SANS data (κ_bare) underwent only a minor decrease. Noting that κ_bare reflects contributions from both the elasticity and saddle-splay moduli, this suggests that κ_bare is not an effective indicator of dynamics for the BμEs investigated. Our study demonstrates that amphiphilic solubilizates can have a complex impact on dynamics of surfactant monolayers in microemulsions, with implications for transmembrane diffusion and the kinetics of release from the microemulsion phase, which are important characteristics for the use of BμEs as delivery vehicles and host systems for (bio)chemical reactions.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Louisiana State Univ., Baton Rouge, LA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0012432

OSTI ID:: 1615940

Journal Information:: Journal of Physical Chemistry. C, Vol. 123, Issue 17; ISSN 1932-7447

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 10 works

Citation information provided by
Web of Science

References (49)

Process of inducing pores in membranes by melittin Lee, M. -T.; Sun, T. -L.; Hung, W. -C. Proceedings of the National Academy of Sciences, Vol. 110, Issue 35 https://doi.org/10.1073/pnas.1307010110	journal	August 2013
Analysis of Macromolecular Polydispersity in Intensity Correlation Spectroscopy: The Method of Cumulants Koppel, Dennis E. The Journal of Chemical Physics, Vol. 57, Issue 11 https://doi.org/10.1063/1.1678153	journal	December 1972
Making Sense of Brownian Motion: Colloid Characterization by Dynamic Light Scattering Hassan, Puthusserickal A.; Rana, Suman; Verma, Gunjan Langmuir, Vol. 31, Issue 1 https://doi.org/10.1021/la501789z	journal	July 2014
Dynamics of Phospholipid Membranes beyond Thermal Undulations Gupta, Sudipta; De Mel, Judith U.; Perera, Rasangi M. The Journal of Physical Chemistry Letters, Vol. 9, Issue 11 https://doi.org/10.1021/acs.jpclett.8b01008	journal	May 2018
Shape and size fluctuations of microemulsion droplets: The role of cosurfactant Farago, B.; Richter, D.; Huang, J. S. Physical Review Letters, Vol. 65, Issue 26 https://doi.org/10.1103/physrevlett.65.3348	journal	December 1990
Role of lipids in the interaction of antimicrobial peptides with membranes Teixeira, Vitor; Feio, Maria J.; Bastos, Margarida Progress in Lipid Research, Vol. 51, Issue 2 https://doi.org/10.1016/j.plipres.2011.12.005	journal	April 2012
Bending moduli of microemulsions; comparison of results from small angle neutron scattering and neutron spin-echo spectroscopy Monkenbusch, M.; Holderer, O.; Frielinghaus, H. Journal of Physics: Condensed Matter, Vol. 17, Issue 31 https://doi.org/10.1088/0953-8984/17/31/017	journal	July 2005
Phase Behavior of Ionic Microemulsions Lekkerkerker, H. N. W.; Kegel, W. K.; Overbeek, J. Th. G. Berichte der Bunsengesellschaft für physikalische Chemie, Vol. 100, Issue 3 https://doi.org/10.1002/bbpc.19961000305	journal	March 1996
The role of electrostatic interactions in the membrane binding of melittin Hall, Kristopher; Lee, Tzong-Hsien; Aguilar, Marie-Isabel Journal of Molecular Recognition, Vol. 24, Issue 1 https://doi.org/10.1002/jmr.1032	journal	March 2010
The high-resolution neutron spin-echo spectrometer for the SNS with τ⩾1μs Ohl, M.; Monkenbusch, M.; Richter, D. Physica B: Condensed Matter, Vol. 350, Issue 1-3 https://doi.org/10.1016/j.physb.2004.04.014	journal	July 2004
Contribution of the electric double layer to the curvature elasticity of charged amphiphilic monolayers Lekkerkerker, H. N. W. Physica A: Statistical Mechanics and its Applications, Vol. 159, Issue 3 https://doi.org/10.1016/0378-4371(89)90400-7	journal	September 1989
Antimicrobial Peptide Action on Parasites Torrent, Marc; Pulido, David; Rivas, Luis Current Drug Targets, Vol. 13, Issue 9 https://doi.org/10.2174/138945012802002393	journal	July 2012
Protein extraction by Winsor-III microemulsion systems Gomez del Rio, Javier A.; Hayes, Douglas G. Biotechnology Progress, Vol. 27, Issue 4 https://doi.org/10.1002/btpr.611	journal	June 2011
Observation of a Topological Relaxation Mode in Microemulsions Peter, U.; Roux, D.; Sood, A. K. Physical Review Letters, Vol. 86, Issue 15 https://doi.org/10.1103/physrevlett.86.3340	journal	April 2001
Amphiphilic block copolymers in oil-water-surfactant mixtures: efficiency boosting, structure, phase behaviour and mechanism Gompper, G.; Richter, D.; Strey, R. Journal of Physics: Condensed Matter, Vol. 13, Issue 41 https://doi.org/10.1088/0953-8984/13/41/302	journal	September 2001
Viscosity of deuterium oxide and water in the range 5 to 125 C Hardy, Robert C.; Cottington, Robert L. Journal of Research of the National Bureau of Standards, Vol. 42, Issue 6 https://doi.org/10.6028/jres.042.049	journal	June 1949
Bending elasticity of the surfactant monolayer in droplet microemulsions: Determination by a combination of dynamic light scattering and neutron spin-echo spectroscopy Hellweg, Thomas; Langevin, Dominique Physical Review E, Vol. 57, Issue 6 https://doi.org/10.1103/physreve.57.6825	journal	June 1998
Removal of non-ionic organic pollutants from water via liquid–liquid extraction López-Montilla, Juan C.; Pandey, Samir; Shah, Dinesh O. Water Research, Vol. 39, Issue 9 https://doi.org/10.1016/j.watres.2005.02.018	journal	May 2005
APD3: the antimicrobial peptide database as a tool for research and education Wang, Guangshun; Li, Xia; Wang, Zhe Nucleic Acids Research, Vol. 44, Issue D1 https://doi.org/10.1093/nar/gkv1278	journal	November 2015
Effect of Protein Incorporation on the Nanostructure of the Bicontinuous Microemulsion Phase of Winsor-III Systems: A Small-Angle Neutron Scattering Study Hayes, Douglas G.; Gomez del Rio, Javier A.; Ye, Ran Langmuir, Vol. 31, Issue 6 https://doi.org/10.1021/la504606x	journal	February 2015
Dynamic Light Scattering Study of Concentrated W/O Microemulsions Bellocq, A. M.; Fourche, G.; Chabrat, P. Optica Acta: International Journal of Optics, Vol. 27, Issue 12 https://doi.org/10.1080/713820182	journal	December 1980
Internal Dynamics in SDS Micelles: Neutron Scattering Study Sharma, V. K.; Mitra, S.; Verma, G. The Journal of Physical Chemistry B, Vol. 114, Issue 51 https://doi.org/10.1021/jp108274y	journal	December 2010
Dynamics of bicontinuous microemulsion phases with and without amphiphilic block-copolymers Mihailescu, M.; Monkenbusch, M.; Endo, H. The Journal of Chemical Physics, Vol. 115, Issue 20 https://doi.org/10.1063/1.1413509	journal	November 2001
Interlayer distance dependence of thickness fluctuations in a swollen lamellar phase Nagao, Michihiro; Chawang, Sukhum; Hawa, Takumi Soft Matter, Vol. 7, Issue 14 https://doi.org/10.1039/c1sm05477e	journal	January 2011
The membrane interactions of antimicrobial peptides revealed by solid-state NMR spectroscopy Bechinger, Burkhard; Salnikov, Evgeniy S. Chemistry and Physics of Lipids, Vol. 165, Issue 3 https://doi.org/10.1016/j.chemphyslip.2012.01.009	journal	April 2012
Dynamical and Phase Behavior of a Phospholipid Membrane Altered by an Antimicrobial Peptide at Low Concentration Sharma, V. K.; Mamontov, E.; Tyagi, M. The Journal of Physical Chemistry Letters, Vol. 7, Issue 13 https://doi.org/10.1021/acs.jpclett.6b01006	journal	June 2016
Confinement Effects in Block Copolymer Modified Bicontinuous Microemulsions Maccarrone, Simona; Byelov, Dmytro V.; Auth, Thorsten The Journal of Physical Chemistry B, Vol. 117, Issue 18 https://doi.org/10.1021/jp402825d	journal	April 2013
Dynamics of ternary polymer blends: Disordered, ordered and bicontinuous microemulsion phases Morkved, Terry L.; Chapman, Bryan R.; Bates, Frank S. Faraday Discussions, Vol. 112 https://doi.org/10.1039/a809169b	journal	January 1999
Scattering intensity of bicontinuous microemulsions and sponge phases Peltomäki, Matti; Gompper, Gerhard; Kroll, Daniel M. The Journal of Chemical Physics, Vol. 136, Issue 13 https://doi.org/10.1063/1.3701265	journal	April 2012
Melittin peptide kills Trypanosoma cruzi parasites by inducing different cell death pathways Adade, Camila M.; Oliveira, Isabelle R. S.; Pais, Joana A. R. Toxicon, Vol. 69 https://doi.org/10.1016/j.toxicon.2013.03.011	journal	July 2013
Bicontinuous microemulsions as a biomembrane mimetic system for melittin Hayes, Douglas G.; Ye, Ran; Dunlap, Rachel N. Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1860, Issue 2 https://doi.org/10.1016/j.bbamem.2017.11.005	journal	February 2018
Detergent-like actions of linear amphipathic cationic antimicrobial peptides Bechinger, Burkhard; Lohner, Karl Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1758, Issue 9 https://doi.org/10.1016/j.bbamem.2006.07.001	journal	September 2006
Small‐angle neutron scattering from microemulsions near the disorder line in water/formamide–octane‐C _i E _j systems Schubert, K. ‐V.; Strey, R. The Journal of Chemical Physics, Vol. 95, Issue 11 https://doi.org/10.1063/1.461282	journal	December 1991
Measuring bending rigidity and spatial renormalization in bicontinuous microemulsions Gompper, G.; Endo, H.; Mihailescu, M. Europhysics Letters (EPL), Vol. 56, Issue 5 https://doi.org/10.1209/epl/i2001-00574-3	journal	December 2001
Triacylglycerol microemulsions stabilized by alkyl ethoxylate surfactants—A basic study Engelskirchen, S.; Elsner, N.; Sottmann, T. Journal of Colloid and Interface Science, Vol. 312, Issue 1 https://doi.org/10.1016/j.jcis.2006.09.022	journal	August 2007
Experimental determination of bending rigidity and saddle splay modulus in bicontinuous microemulsions Holderer, Olaf; Frielinghaus, Henrich; Monkenbusch, Michael Soft Matter, Vol. 9, Issue 7 https://doi.org/10.1039/c2sm27449c	journal	January 2013
Correlation of high-pressure diffusion and viscosity coefficients for n-alkanes Dymond, J. H.; Awan, M. A. International Journal of Thermophysics, Vol. 10, Issue 5 https://doi.org/10.1007/bf00503163	journal	September 1989
Silicone Microemulsion Structures Are Maintained During Polymerization with Reactive Surfactants Whinton, Marlena; Hughes, Timothy C.; Peng, Shuhua Langmuir, Vol. 34, Issue 14 https://doi.org/10.1021/acs.langmuir.8b00240	journal	March 2018
Protein extraction into the bicontinuous microemulsion phase of a Water/SDS/pentanol/dodecane winsor-III system: Effect on nanostructure and protein conformation Hayes, Douglas G.; Ye, Ran; Dunlap, Rachel N. Colloids and Surfaces B: Biointerfaces, Vol. 160 https://doi.org/10.1016/j.colsurfb.2017.09.005	journal	December 2017
Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? Brogden, Kim A. Nature Reviews Microbiology, Vol. 3, Issue 3 https://doi.org/10.1038/nrmicro1098	journal	March 2005
Neutron Spin Echo in Polymer Systems, Chapter 1 Richter, Dieter; Monkenbusch, Michael; Arbe, Arantxa Neutron Spin Echo in Polymer Systems https://doi.org/10.1007/b99835	book	January 2005
The Anti-Arthritic Effects of Synthetic Melittin on the Complete Freund's Adjuvant-Induced Rheumatoid Arthritis Model in Rats Li, Jinghua; Ke, Tao; He, Chao The American Journal of Chinese Medicine, Vol. 38, Issue 06 https://doi.org/10.1142/s0192415x10008457	journal	January 2010
UV-LED induced bicontinuous microemulsions polymerisation for surface modification of commercial membranes – Enhancing the antifouling properties Galiano, Francesco; André Schmidt, Stefan; Ye, Xiaoyun Separation and Purification Technology, Vol. 194 https://doi.org/10.1016/j.seppur.2017.10.063	journal	April 2018
The spin-echo spectrometer at the Spallation Neutron Source (SNS) Ohl, M.; Monkenbusch, M.; Arend, N. Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 696 https://doi.org/10.1016/j.nima.2012.08.059	journal	December 2012
Small angle neutron scattering near Lifshitz lines: Transition from weakly structured mixtures to microemulsions Schubert, K. ‐V.; Strey, R.; Kline, S. R. The Journal of Chemical Physics, Vol. 101, Issue 6 https://doi.org/10.1063/1.467387	journal	September 1994
Segregation of Amphiphilic Polymer-Coated Nanoparticles to Bicontinuous Oil/Water Microemulsion Phases Qi, Luqing; ShamsiJazeyi, Hadi; Ruan, Gedeng Energy & Fuels, Vol. 31, Issue 2 https://doi.org/10.1021/acs.energyfuels.6b02687	journal	February 2017
Dynamic properties of microemulsions modified with homopolymers and diblock copolymers: The determination of bending moduli and renormalization effects Holderer, Olaf; Frielinghaus, Henrich; Byelov, Dmytro The Journal of Chemical Physics, Vol. 122, Issue 9 https://doi.org/10.1063/1.1857523	journal	March 2005
Melittin restores proteasome function in an animal model of ALS Yang, Eun Jin; Kim, Seon Hwy; Yang, Sun Choel Journal of Neuroinflammation, Vol. 8, Issue 1 https://doi.org/10.1186/1742-2094-8-69	journal	January 2011
The structure of melittin. II. Interpretation of the structure. Terwilliger, T. C.; Eisenberg, D. Journal of Biological Chemistry, Vol. 257, Issue 11 https://doi.org/10.1016/S0021-9258(20)65098-0	journal	June 1982

Similar Records

Protein extraction into the bicontinuous microemulsion phase of a Water/SDS/pentanol/dodecane winsor-III system: Effect on nanostructure and protein conformation

Journal Article · Thu Sep 07 00:00:00 EDT 2017 · Colloids and Surfaces. B, Biointerfaces · OSTI ID:1615940

Hayes, Douglas G.; Ye, Ran; Dunlap, Rachel N.; +4 more

Assessment of antimicrobial activity of melittin encapsulated in bicontinuous microemulsions prepared using renewable oils

Journal Article · Wed Dec 28 00:00:00 EST 2022 · Journal of Surfactants and Detergents · OSTI ID:1615940

Oehler, Madison A.; Hayes, Douglas G.; D'Souza, Doris H.; +6 more

Incorporation of Membrane Proteins Into Bicontinuous Microemulsions Through Winsor‐III System‐Based Extraction

Journal Article · Sun Mar 14 00:00:00 EST 2021 · Journal of Surfactants and Detergents · OSTI ID:1615940

Hayes, Douglas G.; Anunciado, Divina B.; Ye, Ran; +4 more

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
Lipids
Sodium dodecyl sulfate
Dynamic light scattering
Monolayers
Surfactants
antimicrobial peptides
bicontinuous microemulsions
dynamics
collective motions
elasticity
bending modulus
neutron spin echo
small-angle neutron scattering
dynamic light scattering
sodium dodecyl sulfate
winsor-III systems
melittin

Title: Incorporation of Melittin Enhances Interfacial Fluidity of Bicontinuous Microemulsions

Citation Formats

References (49)

Similar Records

Related Subjects