Metabolic engineering of Escherichia coli for the production of butyric acid at high titer and productivity

Wang, Liang; Chauliac, Diane; Moritz, Brelan E.; Zhang, Guimin; Ingram, Lonnie O.; Shanmugam, K. T.

doi:10.1186/s13068-019-1408-9

Title: Metabolic engineering of Escherichia coli for the production of butyric acid at high titer and productivity

Journal Article · Fri Mar 22 00:00:00 EDT 2019 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-019-1408-9· OSTI ID:1613814

Wang, Liang ^[1]; Chauliac, Diane ^[1]; Moritz, Brelan E. ^[1]; Zhang, Guimin ^[2]; Ingram, Lonnie O. ^[1]; Shanmugam, K. T. ^[1]

Univ. of Florida, Gainesville, FL (United States)
Univ. of Florida, Gainesville, FL (United States); Hubei Univ., Wuhan (China)

Background: Several anaerobic bacteria produce butyric acid, a commodity chemical with use in chemical, pharmaceutical, food and feed industries, using complex media with acetate as a co- product. Butyrate titer of various recombinant Escherichia coli did not exceed 10 g.l-1 in batch fermentations in any of the media tested. Results: A recombinant E. coli (strain LW393) that produced butyrate as the major fermentation product was constructed with genes from E. coli, Clostridium acetobutylicum and Treponema denticola. Strain LW393 produced 323 ± 6 mM (28.4 ± 0.4 g.l-1) butyric acid in batch fermentations in mineral salts medium with glucose as C-source at a yield of 0.37 ± 0.01 g.(g -1 glucose consumed) Supplementing this medium with yeast extract further increased butyric acid titer to 375 ± 4 mM. . Butyrate accounted for 90% of the total products produced by the culture. -1 -1 Average volumetric productivity of butyrate with xylose as C-source was 0.89 ± 0.07 g.l .h . Conclusions: The butyrate titer reported in this study is about 2.5-3-times higher than the values reported for other recombinant E. coli and this is achieved in mineral salts medium with an expectation of lower purification and production cost of butyrate.

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Research Organization:: Univ. of Florida, Gainesville, FL (United States)

Sponsoring Organization:: USDOE Office of Policy and International Affairs (PO); US Department of Agriculture (USDA); USDOE Office of International Affairs (IA)

Grant/Contract Number:: PI0000031; 2012-67009-19596; 2011-10006-30358

OSTI ID:: 1613814

Alternate ID(s):: OSTI ID: 1766300

Journal Information:: Biotechnology for Biofuels, Vol. 12, Issue 1; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 19 works

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
Biotechnology & Applied Microbiology
Energy & Fuels
Butyrate
Fermentation
E. coli
Xylose
Mineral salt medium
xylose
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MOESM1 of Metabolic engineering of Escherichia coli for the production of butyric acid at high titer and productivity Wang, Liang; Chauliac, Diane; Moritz, Brelan https://doi.org/10.6084/m9.figshare.7884128.v1 The current record is supplemented by this text	text	January 2019
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Metabolic engineering of Escherichia coli for the production of butyric acid at high titer and productivity Wang, Liang; Chauliac, Diane; Moritz, Brelan https://doi.org/10.6084/m9.figshare.c.4445720.v1 The current record is supplemented by this collection	collection	January 2019
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MOESM1 of Metabolic engineering of Rhodopseudomonas palustris for the obligate reduction of n-butyrate to n-butanol Doud, Devin; Holmes, Eric; Richter, Hanno https://doi.org/10.6084/m9.figshare.c.3823291_d1 The current record is supplemented by this text	text	January 2017
MOESM2 of Metabolic engineering of Rhodopseudomonas palustris for the obligate reduction of n-butyrate to n-butanol Doud, Devin; Holmes, Eric; Richter, Hanno https://doi.org/10.6084/m9.figshare.c.3823291_d2 The current record is supplemented by this text	text	January 2017
MOESM3 of Metabolic engineering of Rhodopseudomonas palustris for the obligate reduction of n-butyrate to n-butanol Doud, Devin; Holmes, Eric; Richter, Hanno https://doi.org/10.6084/m9.figshare.c.3823291_d3 The current record is supplemented by this text	text	January 2017
MOESM4 of Metabolic engineering of Rhodopseudomonas palustris for the obligate reduction of n-butyrate to n-butanol Doud, Devin; Holmes, Eric; Richter, Hanno https://doi.org/10.6084/m9.figshare.c.3823291_d4 The current record is supplemented by this text	text	January 2017
MOESM5 of Metabolic engineering of Rhodopseudomonas palustris for the obligate reduction of n-butyrate to n-butanol Doud, Devin; Holmes, Eric; Richter, Hanno https://doi.org/10.6084/m9.figshare.c.3823291_d5.v1 The current record is supplemented by this text	text	January 2017
MOESM6 of Metabolic engineering of Rhodopseudomonas palustris for the obligate reduction of n-butyrate to n-butanol Doud, Devin; Holmes, Eric; Richter, Hanno https://doi.org/10.6084/m9.figshare.c.3823291_d6 The current record is supplemented by this text	text	January 2017
MOESM7 of Metabolic engineering of Rhodopseudomonas palustris for the obligate reduction of n-butyrate to n-butanol Doud, Devin; Holmes, Eric; Richter, Hanno https://doi.org/10.6084/m9.figshare.c.3823291_d7 The current record is supplemented by this text	text	January 2017
MOESM8 of Metabolic engineering of Rhodopseudomonas palustris for the obligate reduction of n-butyrate to n-butanol Doud, Devin; Holmes, Eric; Richter, Hanno https://doi.org/10.6084/m9.figshare.c.3823291_d8.v1 The current record is supplemented by this text	text	January 2017
MOESM1 of Metabolic engineering of Escherichia coli for the production of butyric acid at high titer and productivity Wang, Liang; Chauliac, Diane; Moritz, Brelan https://doi.org/10.6084/m9.figshare.7884128 The current record is supplemented by this text	text	January 2019