Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Population structure of Miscanthus sacchariflorus reveals two major polyploidization events, tetraploid-mediated unidirectional introgression from diploid M. sinensis, and diversity centred around the Yellow Sea

Journal Article · · Annals of Botany
DOI:https://doi.org/10.1093/aob/mcy161· OSTI ID:1610876
 [1];  [2];  [3];  [4];  [5];  [5];  [6];  [5];  [5];  [7];  [8];  [9];  [9];  [4];  [10];  [5];  [4];  [7];  [7];  [1] more »;  [1] « less
  1. Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, USA
  2. Agronomy Department, Key Laboratory of Crop Germplasm Research of Zhejiang Province, Zhejiang University, Hangzhou, China
  3. Department of Food Science, Aarhus University, Årslev, Denmark
  4. Field Science Center for Northern Biosphere, Hokkaido University, Sapporo, Hokkaido, Japan
  5. Vavilov All-Russian Institute of Plant Genetic Resources, Russia
  6. TINPLANT Biotechnik und Pflanzenvermehrung GmbH, Klein Wanzleben, Germany
  7. Kangwon National University, Chuncheon, Gangwon, South Korea
  8. USDA-ARS Forage and Range Research Lab, Utah State University, Logan, UT, USA
  9. Department of Agroecology, Aarhus University, Tjele, Denmark
  10. Life Science and Technology Center, China National Seed Group Co. Ltd, Wuhan, Hubei, China
+ Show Author Affiliations

Abstract Background and Aims

Miscanthus, a C4 perennial grass native to East Asia, is a promising biomass crop. Miscanthus sacchariflorus has a broad geographic range, is used to produce paper in China and is one of the parents (along with Miscanthus sinensis) of the important biomass species Miscanthus × giganteus. The largest study of M. sacchariflorus population genetics to date is reported here.

Methods

Collections included 764 individuals across East Asia. Samples were genotyped with 34 605 single nucleotide polymorphisms (SNPs) derived from restriction site-associated DNA sequencing (RAD-seq) and ten plastid microsatellites, and were subjected to ploidy analysis by flow cytometry.

Key Results

Six major genetic groups within M. sacchariflorus were identified using SNP data: three diploid groups, comprising Yangtze (M. sacchariflorus ssp. lutarioriparius), N China and Korea/NE China/Russia; and three tetraploid groups, comprising N China/Korea/Russia, S Japan and N Japan. Miscanthus sacchariflorus ssp. lutarioriparius was derived from the N China group, with a substantial bottleneck. Japanese and mainland tetraploids originated from independent polyploidization events. Hybrids between diploid M. sacchariflorus and M. sinensis were identified in Korea, but without introgression into either parent species. In contrast, tetraploid M. sacchariflorus in southern Japan and Korea exhibited substantial hybridization and introgression with local diploid M. sinensis.

Conclusions

Genetic data indicated that the land now under the Yellow Sea was a centre of diversity for M. sacchariflorus during the last glacial maximum, followed by a series of migrations as the climate became warmer and wetter. Overall, M. sacchariflorus has greater genetic diversity than M. sinensis, suggesting that breeding and selection within M. sacchariflorus will be important for the development of improved M. × giganteus. Ornamental M. sacchariflorus genotypes in Europe and North America represent a very narrow portion of the species’ genetic diversity, and thus do not well represent the species as a whole.

Research Organization:
Univ. of Illinois at Urbana-Champaign, IL (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
DOE Contract Number:
SC0006634; SC0012379
OSTI ID:
1610876
Journal Information:
Annals of Botany, Vol. 124, Issue 4; ISSN 0305-7364
Publisher:
Oxford University Press
Country of Publication:
United States
Language:
English

References (50)

Preliminary Vegetation Maps of the World since the Last Glacial Maximum: An Aid to Archaeological Understanding July 1997
Accounting for genotype uncertainty in the estimation of allele frequencies in autopolyploids December 2015
TESS3: fast inference of spatial population structure and genome scans for selection October 2015
Chloroplast DNA markers (cpSSRs, SNPs) for Miscanthus, Saccharum and related grasses (Panicoideae, Poaceae) May 2010
Plant morphology, genome size, and SSR markers differentiate five distinct taxonomic groups among accessions in the genus Miscanthus January 2014
A footprint of past climate change on the diversity and population structure of Miscanthus sinensis June 2014
Ecological characteristics and in situ genetic associations for yield-component traits of wild Miscanthus from eastern Russia July 2016
Genetic structure of Miscanthus sinensis and Miscanthus sacchariflorus in Japan indicates a gradient of bidirectional but asymmetric introgression January 2015
Overwintering problems of newly established Miscanthus plantations can be overcome by identifying genotypes with improved rhizome cold tolerance: RESEARCH Miscanthus rhizome frost tolerance November 2000
DIYABC v2.0: a software to make approximate Bayesian computation inferences about population history using single nucleotide polymorphism, DNA sequence and microsatellite data January 2014
More Productive Than Maize in the Midwest: How Does Miscanthus Do It? June 2009
Genetic mapping of biomass yield in three interconnected Miscanthus populations August 2017
Genetic Analysis of Putative Triploid Miscanthus Hybrids and Tetraploid M. sacchariflorus Collected from Sympatric Populations of Kushima, Japan October 2012
Detecting the number of clusters of individuals using the software structure: a simulation study July 2005
Genotypic variation in cold tolerance influences the yield of Miscanthus December 2006
Estimating allelic richness and its diversity May 2006
Microprep protocol for extraction of DNA from tomato and other herbaceous plants September 1995
Androgenesis in the Bioenergy Plant : From Calli Induction to Plant Regeneration January 2012
Variation in chilling tolerance for photosynthesis and leaf extension growth among genotypes related to the C4 grass Miscanthus ×giganteus July 2014
Genetic variation in Miscanthus × giganteus and the importance of estimating genetic distance thresholds for differentiating clones June 2014
Meeting US biofuel goals with less land: the potential of Miscanthus September 2008
Nomenclature of Miscanthus xgiganteus (Poaceae) January 2001
The use of dna sequencing (ITS and trnL-F), AFLP, and fluorescent in situ hybridization to study allopolyploid Miscanthus (Poaceae) February 2002
Miscanthus : a case study for the utilization of natural genetic variation November 2014
Molecular evidence for a natural diploid hybrid between Miscanthus sinensis (Poaceae) and M. sacchariflorus April 2013
Development of novel chloroplast microsatellite markers for Miscanthus species (Poaceae) May 2012
Discriminant analysis of principal components: a new method for the analysis of genetically structured populations January 2010
G ST and its relatives do not measure differentiation September 2008
Nuclear DNA content variation of three Miscanthus species in China January 2013
Cytogenetic Analysis of Miscanthus‘Giganteus’, an Interspecific Hybrid December 1993
Switchgrass Genomic Diversity, Ploidy, and Evolution: Novel Insights from a Network-Based SNP Discovery Protocol January 2013
Meiotic recombination and genome evolution in plants April 2016
Diversity in ploidy levels and nuclear DNA amounts in Korean Miscanthus species June 2013
Analysis of Gene Diversity in Subdivided Populations December 1973
Discovery of natural Miscanthus (Poaceae) triploid plants in sympatric populations of Miscanthus sacchariflorus and Miscanthus sinensis in southern Japan December 2010
pegas: an R package for population genetics with an integrated-modular approach January 2010
APE: Analyses of Phylogenetics and Evolution in R language January 2004
Inference of Population Splits and Mixtures from Genome-Wide Allele Frequency Data November 2012
Genome Size of Three Miscanthus Species October 2008
The Gene Pool of Miscanthus Species and Its Improvement July 2012
Nuclear DNA content in Miscanthus sp. and the geographical variation pattern in Miscanthus lutarioriparius October 2016
Feedstocks for Lignocellulosic Biofuels August 2010
Carl Johann Maximowicz March 1891
A taxonomic revision of Miscanthus s.l. (Poaceae) from China: REVISION OF MISCANTHUS October 2010
Discovery of Natural Interspecific Hybrids Between Miscanthus Sacchariflorus and Miscanthus Sinensis in Southern Japan: Morphological Characterization, Genetic Structure, and Origin November 2015
Naturally occurring triploid hybrids between Miscanthus sacchariflorus and M. sinensis in Southern Japan, show phenotypic variation in agronomic and morphological traits August 2016
mmod : an R library for the calculation of population differentiation statistics August 2012
Genetic variation and bidirectional gene flow in the riparian plant Miscanthus lutarioriparius , across its endemic range: implications for adaptive potential July 2015
Seismic stratigraphy and depositional history of late Quaternary deposits in a tide-dominated setting: An example from the eastern Yellow Sea May 2016
Assessment of genetic diversity of Korean Miscanthus using morphological traits and SSR markers July 2014

Similar Records

Related Subjects

Plant Sciences